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Journal of Virology, May 1999, p. 4447-4451, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Type 1 CD4+ T-Cell Help Is Required for Induction of Antipeptide Multispecific Cytotoxic T Lymphocytes by a Lipopeptidic Vaccine in Rhesus Macaques

Lorenzo Mortara,1,* Helene Gras-Masse,2 Corinne Rommens,2 Alain Venet,1 Jean-Gerard Guillet,1 and Isabelle Bourgault-Villada1

Institut Cochin de Génétique Moléculaire (ICGM), Laboratoire d'Immunologie des Pathologies Infectieuses et Tumorales, INSERM U445---Université René Descartes, Hôpital Cochin, 75014 Paris,1 and Laboratoire Synthèse, Structure et Fonction des Biomolécules, URA CNRS 1309, Institut de Biologie de Lille---Université de Lille II, Institut Pasteur de Lille, 59021 Lille Cedex,2 France

Received 26 June 1998/Accepted 21 January 1999

We have optimized the induction of antiviral cytotoxic T lymphocytes (CTL) in rhesus macaques by a lipopeptide vaccine containing seven peptides from simian immunodeficiency virus (SIV) Nef and Gag proteins and a strong T-helper peptide from tetanus toxoid (TT) that is promiscuous in humans (peptide TT 830-846). Two of the eight immunized macaques showed T-helper (Th) cell proliferation and a specific synthesis of gamma interferon in response to TT 830-846 peptide. They also showed multispecific cytotoxic activity against three to five of the immunizing SIV peptides. These results show the importance of a strong specific type 1 Th response for inducing a multispecific CTL response in vivo, which is essential for the development of an anti-human immunodeficiency virus vaccine.

* Corresponding author. Mailing address: ICGM, INSERM U445, 27 rue du Faubourg Saint-Jacques, 75014 Paris, France. Phone: (33) 01 44 07 18 21. Fax: (33) 01 44 07 14 25. E-mail: mortara{at}cochin.inserm.fr.

Journal of Virology, May 1999, p. 4447-4451, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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