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Journal of Virology, May 1999, p. 3960-3967, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Simultaneous Infection with Retroviruses Pseudotyped with Different Envelope Proteins Bypasses Viral Receptor Interference Associated with Colocalization of gp70 and Target Cells on Fibronectin CH-296

Emily C. MacNeill,1 Helmut Hanenberg,1,dagger Karen E. Pollok,1 Johannes C. M. van der Loo,1,2,Dagger Marti F. A. Bierhuizen,3 Gerard Wagemaker,3 and David A. Williams1,2,*

Section of Pediatric Hematology/Oncology, Herman B Wells Center for Pediatric Research, Riley Hospital for Children,1 and Howard Hughes Medical Institute,2 Indiana University School of Medicine, Indianapolis, Indiana, and Institute of Hematology, Erasmus University Rotterdam, Rotterdam, The Netherlands3

Received 28 October 1998/Accepted 8 February 1999

Several factors are thought to limit the efficiency of retroviral transduction in clinical gene therapy protocols that target hematopoietic stem cells. For example, the level of expression of the amphotropic receptor Pit-2, a phosphate symporter, appears to be low in human and murine hematopoietic stem cells. We have previously demonstrated that transduction of hematopoietic cells in the presence of the fibronectin (FN) fragment CH-296 is extremely efficient (H. Hanenberg, X. L. Xiao, D. Dilloo, K. Hashino, I. Kato, and D. A. Williams, Nat. Med. 2:876-882, 1996). To examine functionally whether the retrovirus receptor is a limiting factor in transduction of hematopoietic cells, we performed competition experiments in the presence of FN CH-296 with retrovirus vectors pseudotyped with the same or a different envelope protein. We demonstrate in both human erythroleukemia (HEL) cells and primary human CD34+ hematopoietic cells inhibition of efficient infection due to receptor interference when two vectors targeting the amphotropic receptor are used simultaneously. Receptor interference lasted up to 24 h. No interference was demonstrated when vectors targeting the amphotropic receptor and the gibbon ape leukemia virus (GALV) receptor Pit-1 were used concurrently. In contrast, simultaneous infection with vectors targeting both Pit-1 and Pit-2 yielded transduction efficiencies consistently higher than with either vector alone in both HEL cells and human CD34+ hematopoietic cells. These data demonstrate that the use of FN CH-296 leads to amphotropic receptor saturation in these cells. Simultaneous infection with vectors targeting both amphotropic and GALV receptors may prove to be of additional benefit in the design of gene therapy protocols.

* Corresponding author. Mailing address: Cancer Research Institute, Howard Hughes Medical Institute, Indiana University School of Medicine, 1044 W. Walnut St., Indianapolis, IN 46202-5225. Phone: (317) 274-8960. Fax: (317) 274-8679. E-mail: dwilliam{at}iupui.edu.

dagger Present address: Department of Pediatric Hematology/Oncology, Heinrich Heine University, Dusseldorf, Germany.

Dagger Present address: University of Minnesota Hospitals, Minneapolis, MN 55455.

Journal of Virology, May 1999, p. 3960-3967, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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