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Journal of Virology, April 1999, p. 3273-3283, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Macrophages Are the Major Reservoir of Latent Murine Gammaherpesvirus 68 in Peritoneal Cells

Karen E. Weck, Susanne S. Kim, Herbert W. Virgin IV,* and Samuel H. Speck*

Department of Pathology and Center for Immunology, Washington University School of Medicine, St. Louis, Missouri 63110

Received 5 October 1998/Accepted 23 December 1998

B cells have previously been identified as the major hematopoietic cell type harboring latent gammaherpesvirus 68 (gamma HV68) (N. P. Sunil-Chandra, S. Efstathiou, and A. A. Nash, J. Gen. Virol. 73:3275-3279, 1992). However, we have shown that gamma HV68 efficiently establishes latency in B-cell-deficient mice (K. E. Weck, M. L. Barkon, L. I. Yoo, S. H. Speck, and H. W. Virgin, J. Virol. 70:6775-6780, 1996), demonstrating that B cells are not required for gamma HV68 latency. To understand this dichotomy, we determined whether hematopoietic cell types, in addition to B cells, carry latent gamma HV68. We observed a high frequency of cells that reactivate latent gamma HV68 in peritoneal exudate cells (PECs) derived from both B-cell-deficient and normal C57BL/6 mice. PECs were composed primarily of macrophages in B-cell-deficient mice and of macrophages plus B cells in normal C57BL/6 mice. To determine which cells in PECs from C57BL/6 mice carry latent gamma HV68, we developed a limiting-dilution PCR assay to quantitate the frequency of cells carrying the gamma HV68 genome in fluorescence-activated cell sorter-purified cell populations. We also quantitated the contribution of individual cell populations to the total frequency of cells carrying latent gamma HV68. At early times after infection, the frequency of PECs that reactivated gamma HV68 correlated very closely with the frequency of PECs carrying the gamma HV68 genome, validating measurement of the frequency of viral-genome-positive cells as a measure of latency in this cell population. F4/80-positive macrophage-enriched, lymphocyte-depleted PECs harbored most of the gamma HV68 genome and efficiently reactivated gamma HV68, while CD19-positive, B-cell-enriched PECs harbored about a 10-fold lower frequency of gamma HV68 genome-positive cells. CD4-positive, T-cell-enriched PECs contained only a very low frequency of gamma HV68 genome-positive cells, consistent with previous analyses indicating that T cells are not a reservoir for gamma HV68 latency (N. P. Sunil-Chandra, S. Efstathiou, and A. A. Nash, J. Gen. Virol. 73:3275-3279, 1992). Since macrophages are bone marrow derived, we determined whether elicitation of a large inflammatory response in the peritoneum would recruit additional latent cells into the peritoneum. Thioglycolate inoculation increased the total number of PECs by about 20-fold but did not affect the frequency of cells that reactivate gamma HV68, consistent with a bone marrow reservoir for latent gamma HV68. These experiments demonstrate gamma HV68 latency in two different hematopoietic cell types, F4/80-positive macrophages and CD19-positive B cells, and argue for a bone marrow reservoir for latent gamma HV68.

* Corresponding author. Mailing address: Department of Pathology, Box 8118, 660 S. Euclid Ave., St. Louis, MO 63110. Phone: H.W.V. (314) 362-9223; S.H.S. (314) 362-0367. Fax: (314) 362-4096. E-mail: virgin{at}pathology.wustl.edu or speck{at}pathology.wustl.edu.

Journal of Virology, April 1999, p. 3273-3283, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Flano, E., Woodland, D. L., Blackman, M. A., Doherty, P. C. (2001). Analysis of Virus-Specific CD4+ T Cells during Long-Term Gammaherpesvirus Infection. J. Virol. 75: 7744-7748 [Abstract] [Full Text]  
  • Bridgeman, A., Stevenson, P. G., Simas, J. P., Efstathiou, S. (2001). A Secreted Chemokine Binding Protein Encoded by Murine Gammaherpesvirus-68 Is Necessary for the Establishment of a Normal Latent Load. JEM 194: 301-312 [Abstract] [Full Text]  
  • Topham, D. J., Cardin, R. C., Christensen, J. P., Brooks, J. W., Belz, G. T., Doherty, P. C. (2001). Perforin and Fas in murine gammaherpesvirus-specific CD8+ T cell control and morbidity. J. Gen. Virol. 82: 1971-1981 [Abstract] [Full Text]  
  • Adler, H., Messerle, M., Koszinowski, U. H. (2001). Virus Reconstituted from Infectious Bacterial Artificial Chromosome (BAC)-Cloned Murine Gammaherpesvirus 68 Acquires Wild-Type Properties In Vivo Only after Excision of BAC Vector Sequences. J. Virol. 75: 5692-5696 [Abstract] [Full Text]  
  • Rochford, R., Lutzke, M. L., Alfinito, R. S., Clavo, A., Cardin, R. D. (2001). Kinetics of Murine Gammaherpesvirus 68 Gene Expression following Infection of Murine Cells in Culture and in Mice. J. Virol. 75: 4955-4963 [Abstract] [Full Text]  
  • Macrae, A. I., Dutia, B. M., Milligan, S., Brownstein, D. G., Allen, D. J., Mistrikova, J., Davison, A. J., Nash, A. A., Stewart, J. P. (2001). Analysis of a Novel Strain of Murine Gammaherpesvirus Reveals a Genomic Locus Important for Acute Pathogenesis. J. Virol. 75: 5315-5327 [Abstract] [Full Text]  
  • Wakeling, M. N., Roy, D. J., Nash, A. A., Stewart, J. P. (2001). Characterization of the murine gammaherpesvirus 68 ORF74 product: a novel oncogenic G protein-coupled receptor. J. Gen. Virol. 82: 1187-1197 [Abstract] [Full Text]  
  • Terry, L. A., Stewart, J. P., Nash, A. A., Fazakerley, J. K. (2000). Murine gammaherpesvirus-68 infection of and persistence in the central nervous system. J. Gen. Virol. 81: 2635-2643 [Abstract] [Full Text]  
  • Usherwood, E. J., Roy, D. J., Ward, K., Surman, S. L., Dutia, B. M., Blackman, M. A., Stewart, J. P., Woodland, D. L. (2000). Control of Gammaherpesvirus Latency by Latent Antigen-Specific Cd8+ T Cells. JEM 192: 943-952 [Abstract] [Full Text]  
  • van Dyk, L. F., Virgin, H. W. IV, Speck, S. H. (2000). The Murine Gammaherpesvirus 68 v-Cyclin Is a Critical Regulator of Reactivation from Latency. J. Virol. 74: 7451-7461 [Abstract] [Full Text]  
  • van Berkel, V., Barrett, J., Tiffany, H. L., Fremont, D. H., Murphy, P. M., McFadden, G., Speck, S. H., Virgin, H. W. IV (2000). Identification of a Gammaherpesvirus Selective Chemokine Binding Protein That Inhibits Chemokine Action. J. Virol. 74: 6741-6747 [Abstract] [Full Text]  
  • Hoge, A. T., Hendrickson, S. B., Burns, W. H. (2000). Murine Gammaherpesvirus 68 Cyclin D Homologue Is Required for Efficient Reactivation from Latency. J. Virol. 74: 7016-7023 [Abstract] [Full Text]  
  • Hardy, C. L., Silins, S. L., Woodland, David. L., Blackman, M. A. (2000). Murine {gamma}-herpesvirus infection causes V{beta}4-specific CDR3-restricted clonal expansions within CD8+ peripheral blood T lymphocytes. Int Immunol 12: 1193-1204 [Abstract] [Full Text]  
  • Flano, E., Husain, S. M., Sample, J. T., Woodland, D. L., Blackman, M. A. (2000). Latent Murine {gamma}-Herpesvirus Infection Is Established in Activated B Cells, Dendritic Cells, and Macrophages. J. Immunol. 165: 1074-1081 [Abstract] [Full Text]  
  • Lee, B. J., Santee, S., Von Gesjen, S., Ware, C. F., Sarawar, S. R. (2000). Lymphotoxin-alpha -Deficient Mice Can Clear a Productive Infection with Murine Gammaherpesvirus 68 but Fail To Develop Splenomegaly or Lymphocytosis. J. Virol. 74: 2786-2792 [Abstract] [Full Text]  
  • Clambey, E. T., Virgin, H. W. IV, Speck, S. H. (2000). Disruption of the Murine Gammaherpesvirus 68 M1 Open Reading Frame Leads to Enhanced Reactivation from Latency. J. Virol. 74: 1973-1984 [Abstract] [Full Text]  
  • Liu, S., Pavlova, I. V., Virgin, H. W. IV, Speck, S. H. (2000). Characterization of Gammaherpesvirus 68 Gene 50 Transcription. J. Virol. 74: 2029-2037 [Abstract] [Full Text]  
  • Sangster, M. Y., Topham, D. J., D'Costa, S., Cardin, R. D., Marion, T. N., Myers, L. K., Doherty, P. C. (2000). Analysis of the Virus-Specific and Nonspecific B Cell Response to a Persistent B-Lymphotropic Gammaherpesvirus. J. Immunol. 164: 1820-1828 [Abstract] [Full Text]  
  • Parry, C. M., Simas, J. P., Smith, V. P., Stewart, C. A., Minson, A. C., Efstathiou, S., Alcami, A. (2000). A Broad Spectrum Secreted Chemokine Binding Protein Encoded by a Herpesvirus. JEM 191: 573-578 [Abstract] [Full Text]  
  • Peacock, J. W., Bost, K. L. (2000). Infection of intestinal epithelial cells and development of systemic disease following gastric instillation of murine gammaherpesvirus-68. J. Gen. Virol. 81: 421-429 [Abstract] [Full Text]  
  • Liu, L., Usherwood, E. J., Blackman, M. A., Woodland, D. L. (1999). T-Cell Vaccination Alters the Course of Murine Herpesvirus 68 Infection and the Establishment of Viral Latency in Mice. J. Virol. 73: 9849-9857 [Abstract] [Full Text]  


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