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Journal of Virology, April 1999, p. 3210-3218, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Visualization of Tegument-Capsid Interactions and DNA in Intact Herpes Simplex Virus Type 1 Virions

Z. Hong Zhou,1 Dong Hua Chen,2 Joanita Jakana,2 Frazer J. Rixon,3 and Wah Chiu2,*

Department of Pathology and Laboratory Medicine, University of Texas---Houston Medical School,1 and Verna & Marrs McLean Department of Biochemistry, Baylor College of Medicine,2 Houston, Texas 77030, and MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, Scotland, United Kingdom3

Received 29 October 1998/Accepted 4 January 1999

Herpes simplex virus type 1 virions were examined by electron cryomicroscopy, allowing the three-dimensional structure of the infectious particle to be visualized for the first time. The capsid shell is identical to that of B-capsids purified from the host cell nucleus, with the exception of the penton channel, which is closed. The double-stranded DNA genome is organized as regularly spaced (~26 Å) concentric layers inside the capsid. This pattern suggests a spool model for DNA packaging, similar to that for some bacteriophages. The bulk of the tegument is not icosahedrally ordered. However, a small portion appears as filamentous structures around the pentons, interacting extensively with the capsid. Their locations and interactions suggest possible roles for the tegument proteins in regulating DNA transport through the penton channel and binding to cellular transport proteins during viral infection.

* Corresponding author. Mailing address: Verna & Marrs McLean Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498. Phone: (713) 798-6985. Fax: (713) 796-9438. E-mail: wah{at}bcm.tmc.edu.

Journal of Virology, April 1999, p. 3210-3218, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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