This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Didcock, L.
Right arrow Articles by Randall, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Didcock, L.
Right arrow Articles by Randall, R. E.

 Previous Article  |  Next Article 

Journal of Virology, April 1999, p. 3125-3133, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Sendai Virus and Simian Virus 5 Block Activation of Interferon-Responsive Genes: Importance for Virus Pathogenesis

L. Didcock,1 D. F. Young,1 S. Goodbourn,2 and R. E. Randall1,*

School of Biomedical Sciences, North Haugh University of St. Andrews, Fife, Scotland KY16 9TS,1 and Department of Biochemistry, St. George's Hospital Medical School, University of London, London SW17 0RE, England2

Received 23 October 1998/Accepted 21 December 1998

Sendai virus (SeV) is highly pathogenic for mice. In contrast, mice (including SCID mice) infected with simian virus 5 (SV5) showed no overt signs of disease. Evidence is presented that a major factor which prevented SV5 from productively infecting mice was its inability to circumvent the interferon (IFN) response in mice. Thus, in murine cells that produce and respond to IFN, SV5 protein synthesis was rapidly switched off. In marked contrast, once SeV protein synthesis began, it continued, even if the culture medium was supplemented with alpha/beta IFN (IFN-alpha /beta ). However, in human cells, IFN-alpha /beta did not inhibit the replication of either SV5 or SeV once virus protein synthesis was established. To begin to address the molecular basis for these observations, the effects of SeV and SV5 infections on the activation of an IFN-alpha /beta -responsive promoter and on that of the IFN-beta promoter were examined in transient transfection experiments. The results demonstrated that (i) SeV, but not SV5, inhibited an IFN-alpha /beta -responsive promoter in murine cells; (ii) both SV5 and SeV inhibited the activation of an IFN-alpha /beta -responsive promoter in human cells; and (iii) in both human and murine cells, SeV was a strong inducer of the IFN-beta promoter, whereas SV5 was a poor inducer. The ability of SeV and SV5 to inhibit the activation of IFN-responsive genes in human cells was confirmed by RNase protection experiments. The importance of these results in terms of paramyxovirus pathogenesis is discussed.

* Corresponding author. Mailing address: School of Biomedical Sciences, Biomedical Sciences Bldg., North Haugh University of St. Andrews, Fife, Scotland KY16 9TS. Phone: 44 1334 463397. Fax: 44 1334 462595. E-mail: rer{at}st-and.ac.uk.

Journal of Virology, April 1999, p. 3125-3133, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Feng, N., Sen, A., Nguyen, H., Vo, P., Hoshino, Y., Deal, E. M., Greenberg, H. B. (2009). Variation in Antagonism of the Interferon Response to Rotavirus NSP1 Results in Differential Infectivity in Mouse Embryonic Fibroblasts. J. Virol. 83: 6987-6994 [Abstract] [Full Text]  
  • Nishio, M., Ohtsuka, J., Tsurudome, M., Nosaka, T., Kolakofsky, D. (2008). Human Parainfluenza Virus Type 2 V Protein Inhibits Genome Replication by Binding to the L Protein: Possible Role in Promoting Viral Fitness. J. Virol. 82: 6130-6138 [Abstract] [Full Text]  
  • Randall, R. E., Goodbourn, S. (2008). Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J. Gen. Virol. 89: 1-47 [Abstract] [Full Text]  
  • Dillon, P. J., Parks, G. D. (2007). Role for the Phosphoprotein P Subunit of the Paramyxovirus Polymerase in Limiting Induction of Host Cell Antiviral Responses. J. Virol. 81: 11116-11127 [Abstract] [Full Text]  
  • Cruz, C. D., Palosaari, H., Parisien, J.-P., Devaux, P., Cattaneo, R., Ouchi, T., Horvath, C. M. (2006). Measles Virus V Protein Inhibits p53 Family Member p73.. J. Virol. 80: 5644-5650 [Abstract] [Full Text]  
  • Arimilli, S., Alexander-Miller, M. A., Parks, G. D. (2006). A Simian Virus 5 (SV5) P/V Mutant Is Less Cytopathic than Wild-Type SV5 in Human Dendritic Cells and Is a More Effective Activator of Dendritic Cell Maturation and Function.. J. Virol. 80: 3416-3427 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Ito, Y. (2005). Human Parainfluenza Virus Type 4 Is Incapable of Evading the Interferon-Induced Antiviral Effect. J. Virol. 79: 14756-14768 [Abstract] [Full Text]  
  • Carlos, T. S., Fearns, R., Randall, R. E. (2005). Interferon-Induced Alterations in the Pattern of Parainfluenza Virus 5 Transcription and Protein Synthesis and the Induction of Virus Inclusion Bodies. J. Virol. 79: 14112-14121 [Abstract] [Full Text]  
  • Precious, B., Childs, K., Fitzpatrick-Swallow, V., Goodbourn, S., Randall, R. E. (2005). Simian Virus 5 V Protein Acts as an Adaptor, Linking DDB1 to STAT2, To Facilitate the Ubiquitination of STAT1. J. Virol. 79: 13434-13441 [Abstract] [Full Text]  
  • Hohle, C., Karger, A., Konig, P., Giesow, K., Keil, G. M. (2005). High-level expression of biologically active bovine alpha interferon by Bovine herpesvirus 1 interferes only marginally with recombinant virus replication in vitro. J. Gen. Virol. 86: 2685-2695 [Abstract] [Full Text]  
  • Ulane, C. M., Kentsis, A., Cruz, C. D., Parisien, J.-P., Schneider, K. L., Horvath, C. M. (2005). Composition and Assembly of STAT-Targeting Ubiquitin Ligase Complexes: Paramyxovirus V Protein Carboxyl Terminus Is an Oligomerization Domain. J. Virol. 79: 10180-10189 [Abstract] [Full Text]  
  • Sakaguchi, T., Kato, A., Sugahara, F., Shimazu, Y., Inoue, M., Kiyotani, K., Nagai, Y., Yoshida, T. (2005). AIP1/Alix Is a Binding Partner of Sendai Virus C Protein and Facilitates Virus Budding. J. Virol. 79: 8933-8941 [Abstract] [Full Text]  
  • Openshaw, P. J. M., Tregoning, J. S. (2005). Immune Responses and Disease Enhancement during Respiratory Syncytial Virus Infection. Clin. Microbiol. Rev. 18: 541-555 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Garcin, D., Kolakofsky, D., Ito, Y. (2005). Identification of Paramyxovirus V Protein Residues Essential for STAT Protein Degradation and Promotion of Virus Replication. J. Virol. 79: 8591-8601 [Abstract] [Full Text]  
  • Pejawar, S. S., Parks, G. D., Alexander-Miller, M. A. (2005). Abortive versus Productive Viral Infection of Dendritic Cells with a Paramyxovirus Results in Differential Upregulation of Select Costimulatory Molecules. J. Virol. 79: 7544-7557 [Abstract] [Full Text]  
  • Kubota, T., Yokosawa, N., Yokota, S.-i., Fujii, N., Tashiro, M., Kato, A. (2005). Mumps Virus V Protein Antagonizes Interferon without the Complete Degradation of STAT1. J. Virol. 79: 4451-4459 [Abstract] [Full Text]  
  • Precious, B., Young, D. F., Andrejeva, L., Goodbourn, S., Randall, R. E. (2005). In vitro and in vivo specificity of ubiquitination and degradation of STAT1 and STAT2 by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2. J. Gen. Virol. 86: 151-158 [Abstract] [Full Text]  
  • Chatziandreou, N., Stock, N., Young, D., Andrejeva, J., Hagmaier, K., McGeoch, D. J., Randall, R. E. (2004). Relationships and host range of human, canine, simian and porcine isolates of simian virus 5 (parainfluenza virus 5). J. Gen. Virol. 85: 3007-3016 [Abstract] [Full Text]  
  • Kato, A., Cortese-Grogan, C., Moyer, S. A., Sugahara, F., Sakaguchi, T., Kubota, T., Otsuki, N., Kohase, M., Tashiro, M., Nagai, Y. (2004). Characterization of the Amino Acid Residues of Sendai Virus C Protein That Are Critically Involved in Its Interferon Antagonism and RNA Synthesis Down-Regulation. J. Virol. 78: 7443-7454 [Abstract] [Full Text]  
  • Rodriguez, J. J., Cruz, C. D., Horvath, C. M. (2004). Identification of the Nuclear Export Signal and STAT-Binding Domains of the Nipah Virus V Protein Reveals Mechanisms Underlying Interferon Evasion. J. Virol. 78: 5358-5367 [Abstract] [Full Text]  
  • Thoulouze, M.-I., Bouguyon, E., Carpentier, C., Bremont, M. (2004). Essential Role of the NV Protein of Novirhabdovirus for Pathogenicity in Rainbow Trout. J. Virol. 78: 4098-4107 [Abstract] [Full Text]  
  • Bossert, B., Marozin, S., Conzelmann, K.-K. (2003). Nonstructural Proteins NS1 and NS2 of Bovine Respiratory Syncytial Virus Block Activation of Interferon Regulatory Factor 3. J. Virol. 77: 8661-8668 [Abstract] [Full Text]  
  • Palosaari, H., Parisien, J.-P., Rodriguez, J. J., Ulane, C. M., Horvath, C. M. (2003). STAT Protein Interference and Suppression of Cytokine Signal Transduction by Measles Virus V Protein. J. Virol. 77: 7635-7644 [Abstract] [Full Text]  
  • Munir, S., Kapur, V. (2003). Regulation of Host Cell Transcriptional Physiology by the Avian Pneumovirus Provides Key Insights into Host-Pathogen Interactions. J. Virol. 77: 4899-4910 [Abstract] [Full Text]  
  • Gotoh, B., Takeuchi, K., Komatsu, T., Yokoo, J. (2003). The STAT2 Activation Process Is a Crucial Target of Sendai Virus C Protein for the Blockade of Alpha Interferon Signaling. J. Virol. 77: 3360-3370 [Abstract] [Full Text]  
  • Young, D. F., Andrejeva, L., Livingstone, A., Goodbourn, S., Lamb, R. A., Collins, P. L., Elliott, R. M., Randall, R. E. (2003). Virus Replication in Engineered Human Cells That Do Not Respond to Interferons. J. Virol. 77: 2174-2181 [Abstract] [Full Text]  
  • Neumann, G., Whitt, M. A., Kawaoka, Y. (2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol. 83: 2635-2662 [Abstract] [Full Text]  
  • Andrejeva, J., Poole, E., Young, D. F., Goodbourn, S., Randall, R. E. (2002). The p127 Subunit (DDB1) of the UV-DNA Damage Repair Binding Protein Is Essential for the Targeted Degradation of STAT1 by the V Protein of the Paramyxovirus Simian Virus 5. J. Virol. 76: 11379-11386 [Abstract] [Full Text]  
  • Rodriguez, J. J., Parisien, J.-P., Horvath, C. M. (2002). Nipah Virus V Protein Evades Alpha and Gamma Interferons by Preventing STAT1 and STAT2 Activation and Nuclear Accumulation. J. Virol. 76: 11476-11483 [Abstract] [Full Text]  
  • Wansley, E. K., Parks, G. D. (2002). Naturally Occurring Substitutions in the P/V Gene Convert the Noncytopathic Paramyxovirus Simian Virus 5 into a Virus That Induces Alpha/Beta Interferon Synthesis and Cell Death. J. Virol. 76: 10109-10121 [Abstract] [Full Text]  
  • Weber, F., Bridgen, A., Fazakerley, J. K., Streitenfeld, H., Kessler, N., Randall, R. E., Elliott, R. M. (2002). Bunyamwera Bunyavirus Nonstructural Protein NSs Counteracts the Induction of Alpha/Beta Interferon. J. Virol. 76: 7949-7955 [Abstract] [Full Text]  
  • Kato, A., Ohnishi, Y., Hishiyama, M., Kohase, M., Saito, S., Tashiro, M., Nagai, Y. (2002). The Amino-Terminal Half of Sendai Virus C Protein Is Not Responsible for either Counteracting the Antiviral Action of Interferons or Down-Regulating Viral RNA Synthesis. J. Virol. 76: 7114-7124 [Abstract] [Full Text]  
  • Parisien, J.-P., Lau, J. F., Horvath, C. M. (2002). STAT2 Acts as a Host Range Determinant for Species-Specific Paramyxovirus Interferon Antagonism and Simian Virus 5 Replication. J. Virol. 76: 6435-6441 [Abstract] [Full Text]  
  • Parisien, J.-P., Lau, J. F., Rodriguez, J. J., Ulane, C. M., Horvath, C. M. (2002). Selective STAT Protein Degradation Induced by Paramyxoviruses Requires both STAT1 and STAT2 but Is Independent of Alpha/Beta Interferon Signal Transduction. J. Virol. 76: 4190-4198 [Abstract] [Full Text]  
  • Bossert, B., Conzelmann, K.-K. (2002). Respiratory Syncytial Virus (RSV) Nonstructural (NS) Proteins as Host Range Determinants: a Chimeric Bovine RSV with NS Genes from Human RSV Is Attenuated in Interferon-Competent Bovine Cells. J. Virol. 76: 4287-4293 [Abstract] [Full Text]  
  • Andrejeva, J., Young, D. F., Goodbourn, S., Randall, R. E. (2002). Degradation of STAT1 and STAT2 by the V Proteins of Simian Virus 5 and Human Parainfluenza Virus Type 2, Respectively: Consequences for Virus Replication in the Presence of Alpha/Beta and Gamma Interferons. J. Virol. 76: 2159-2167 [Abstract] [Full Text]  
  • Samuel, C. E. (2001). Antiviral Actions of Interferons. Clin. Microbiol. Rev. 14: 778-809 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Kawano, M., Komada, H., Ito, Y. (2001). High Resistance of Human Parainfluenza Type 2 Virus Protein-Expressing Cells to the Antiviral and Anti-Cell Proliferative Activities of Alpha/Beta Interferons: Cysteine-Rich V-Specific Domain Is Required for High Resistance to the Interferons. J. Virol. 75: 9165-9176 [Abstract] [Full Text]  
  • Garcin, D., Curran, J., Itoh, M., Kolakofsky, D. (2001). Longer and Shorter Forms of Sendai Virus C Proteins Play Different Roles in Modulating the Cellular Antiviral Response. J. Virol. 75: 6800-6807 [Abstract] [Full Text]  
  • He, B., Lin, G. Y., Durbin, J. E., Durbin, R. K., Lamb, R. A. (2001). The SH Integral Membrane Protein of the Paramyxovirus Simian Virus 5 Is Required To Block Apoptosis in MDBK Cells. J. Virol. 75: 4068-4079 [Abstract] [Full Text]  
  • Kato, A., Ohnishi, Y., Kohase, M., Saito, S., Tashiro, M., Nagai, Y. (2001). Y2, the Smallest of the Sendai Virus C Proteins, Is Fully Capable of both Counteracting the Antiviral Action of Interferons and Inhibiting Viral RNA Synthesis. J. Virol. 75: 3802-3810 [Abstract] [Full Text]  
  • Young, D. F., Chatziandreou, N., He, B., Goodbourn, S., Lamb, R. A., Randall, R. E. (2001). Single Amino Acid Substitution in the V Protein of Simian Virus 5 Differentiates Its Ability To Block Interferon Signaling in Human and Murine Cells. J. Virol. 75: 3363-3370 [Abstract] [Full Text]  
  • Wu, Z., Turner, D. R., Oliveira, D. B. G. (2001). IL-4 gene expression up-regulated by mercury in rat mast cells: a role of oxidant stress in IL-4 transcription. Int Immunol 13: 297-304 [Abstract] [Full Text]  
  • Bridgen, A., Weber, F., Fazakerley, J. K., Elliott, R. M. (2001). Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis. Proc. Natl. Acad. Sci. USA 98: 664-669 [Abstract] [Full Text]  
  • Mebatsion, T., Verstegen, S., De Vaan, L. T. C., Römer-Oberdörfer, A., Schrier, C. C. (2001). A Recombinant Newcastle Disease Virus with Low-Level V Protein Expression Is Immunogenic and Lacks Pathogenicity for Chicken Embryos. J. Virol. 75: 420-428 [Abstract] [Full Text]  
  • Lyles, D. S. (2000). Cytopathogenesis and Inhibition of Host Gene Expression by RNA Viruses. Microbiol. Mol. Biol. Rev. 64: 709-724 [Abstract] [Full Text]  
  • Garcin, D., Curran, J., Kolakofsky, D. (2000). Sendai Virus C Proteins Must Interact Directly with Cellular Components To Interfere with Interferon Action. J. Virol. 74: 8823-8830 [Abstract] [Full Text]  
  • Goodbourn, S., Didcock, L., Randall, R. E. (2000). Interferons: cell signalling, immune modulation, antiviral response and virus countermeasures. J. Gen. Virol. 81: 2341-2364 [Full Text]  
  • Schlender, J., Bossert, B., Buchholz, U., Conzelmann, K.-K. (2000). Bovine Respiratory Syncytial Virus Nonstructural Proteins NS1 and NS2 Cooperatively Antagonize Alpha/Beta Interferon-Induced Antiviral Response. J. Virol. 74: 8234-8242 [Abstract] [Full Text]  
  • Hasan, M. K., Kato, A., Muranaka, M., Yamaguchi, R., Sakai, Y., Hatano, I., Tashiro, M., Nagai, Y. (2000). Versatility of the Accessory C Proteins of Sendai Virus: Contribution to Virus Assembly as an Additional Role. J. Virol. 74: 5619-5628 [Abstract] [Full Text]  
  • Komatsu, T., Takeuchi, K., Yokoo, J., Tanaka, Y., Gotoh, B. (2000). Sendai Virus Blocks Alpha Interferon Signaling to Signal Transducers and Activators of Transcription. J. Virol. 74: 2477-2480 [Abstract] [Full Text]  
  • Didcock, L., Young, D. F., Goodbourn, S., Randall, R. E. (1999). The V Protein of Simian Virus 5 Inhibits Interferon Signalling by Targeting STAT1 for Proteasome-Mediated Degradation. J. Virol. 73: 9928-9933 [Abstract] [Full Text]  
  • Garcin, D., Latorre, P., Kolakofsky, D. (1999). Sendai Virus C Proteins Counteract the Interferon-Mediated Induction of an Antiviral State. J. Virol. 73: 6559-6565 [Abstract] [Full Text]  
  • Talon, J., Salvatore, M., O'Neill, R. E., Nakaya, Y., Zheng, H., Muster, T., Garcia-Sastre, A., Palese, P. (2000). Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach. Proc. Natl. Acad. Sci. USA 97: 4309-4314 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»