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Journal of Virology, April 1999, p. 2956-2962, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Antibody Responses to Mature and Immature Forms of Viral Envelope in Respiratory Syncytial Virus Infection: Significance for Subunit Vaccines

Hiroshi Sakurai,1,2 R. Anthony Williamson,1,* James E. Crowe,3 Judy A. Beeler,4 Pascal Poignard,1 Raiza B. Bastidas,1 Robert M. Chanock,5 and Dennis R. Burton1,6,*

Departments of Immunology1 and Molecular Biology,6 The Scripps Research Institute, La Jolla, California 92037; Takasago Institute, Kaneka Corporation, Takasago, Hyogo, Japan 6762; Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 372323; Food and Drug Administration, Rockville, Maryland 208574; Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 208925

Received 19 October 1998/Accepted 11 December 1998

A number of antibodies generated during human respiratory syncytial virus (RSV) infection have been cloned by the phage library approach. Antibodies reactive with an immunodominant epitope on the F glycoprotein of this virus have a high affinity for affinity-purified F antigen. These antibodies, however, have a much lower affinity for mature F glycoprotein on the surface of infected cells and are nonneutralizing. In contrast, a potent neutralizing antibody has a high affinity for mature F protein but a much lower affinity for purified F protein or F protein in viral lysates. The data indicate that at least two F protein immunogens are produced during natural RSV infection: immature F, found in viral lysates, and mature F, found on infected cells or virions. Binding studies with polyclonal human immunoglobulin G suggest that the antibody responses to the two immunogens are of similar magnitudes. Competitive binding studies suggest that overlap between the responses is relatively limited. A mature envelope with an antigenic configuration different from that of the immature envelope has an evolutionary advantage in that the infecting virus is less subject to neutralization by the humoral response to the immature envelope that inevitably arises following lysis of infected cells. Subunit vaccines may be at a disadvantage because they most often resemble immature envelope molecules and ignore this aspect of viral evasion.

* Corresponding author. Mailing address for R. Anthony Williamson: Department of Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-8620. Fax: (619) 784-8360. E-mail: anthony{at}scripps.edu. Mailing address for Dennis R. Burton: Department of Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-9298. Fax: (619) 784-8360. E-mail: burton{at}scripps.edu.

Journal of Virology, April 1999, p. 2956-2962, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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