This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, S.
Right arrow Articles by Nemerow, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, S.
Right arrow Articles by Nemerow, G. R.

 Previous Article  |  Next Article 

Journal of Virology, April 1999, p. 2798-2802, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Single Amino Acid in the Adenovirus Type 37 Fiber Confers Binding to Human Conjunctival Cellsdagger

Shuang Huang, Vijay Reddy, Nabarun Dasgupta, and Glen R. Nemerow*

Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California

Received 18 September 1998/Accepted 15 December 1998

A 46-kDa receptor, coxsackievirus-adenovirus (Ad) receptor (CAR), mediates cell attachment of a number of different Ad serotypes; however, not all Ad serotypes utilize this receptor for infection. Moreover, the precise amino acid sequences in the Ad fiber protein that mediate cell attachment have yet to be identified. We investigated the interaction of subgroup D Ads with human ocular cells. Ad serotype 37 (Ad37), a virus associated with epidemic keratoconjunctivitis, but not a closely related virus serotype, Ad19p, exhibited preferential binding to and infection of human conjunctival cells. A single amino acid substitution in the Ad19p fiber distal domain (knob), Glu240 to Lys, conferred binding to conjunctival cells, while the reverse substitution in the Ad37 fiber abrogated cell binding. These findings provide new information on the fiber sequences that regulate Ad host cell tropism.

* Corresponding author. Mailing address: Department of Immunology, IMM19, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-8072. Fax: (619) 784-8472. E-mail: gnemerow{at}scripps.edu.

dagger Manuscript no. 11946-IMM of The Scripps Research Institute.

Journal of Virology, April 1999, p. 2798-2802, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mathis, J. M., Stewart, P. L., Zhu, Z. B., Curiel, D. T. (2006). Advanced Generation Adenoviral Virotherapy Agents Embody Enhanced Potency Based upon CAR-Independent Tropism.. Clin. Cancer Res. 12: 2651-2656 [Full Text]  
  • Gustafsson, D. J., Segerman, A., Lindman, K., Mei, Y.-F., Wadell, G. (2006). The Arg279Glu Substitution in the Adenovirus Type 11p (Ad11p) Fiber Knob Abolishes EDTA-Resistant Binding to A549 and CHO-CD46 Cells, Converting the Phenotype to That of Ad7p. J. Virol. 80: 1897-1905 [Abstract] [Full Text]  
  • Burmeister, W. P., Guilligay, D., Cusack, S., Wadell, G., Arnberg, N. (2004). Crystal Structure of Species D Adenovirus Fiber Knobs and Their Sialic Acid Binding Sites. J. Virol. 78: 7727-7736 [Abstract] [Full Text]  
  • Wu, E., Trauger, S. A., Pache, L., Mullen, T.-M., Von Seggern, D. J., Siuzdak, G., Nemerow, G. R. (2004). Membrane Cofactor Protein Is a Receptor for Adenoviruses Associated with Epidemic Keratoconjunctivitis. J. Virol. 78: 3897-3905 [Abstract] [Full Text]  
  • Wu, E., Pache, L., Von Seggern, D. J., Mullen, T.-M., Mikyas, Y., Stewart, P. L., Nemerow, G. R. (2003). Flexibility of the Adenovirus Fiber Is Required for Efficient Receptor Interaction. J. Virol. 77: 7225-7235 [Abstract] [Full Text]  
  • Natarajan, K., Rajala, M. S., Chodosh, J. (2003). Corneal IL-8 Expression Following Adenovirus Infection Is Mediated by c-Src Activation in Human Corneal Fibroblasts. J. Immunol. 170: 6234-6243 [Abstract] [Full Text]  
  • Arnberg, N., Pring-Akerblom, P., Wadell, G. (2002). Adenovirus Type 37 Uses Sialic Acid as a Cellular Receptor on Chang C Cells. J. Virol. 76: 8834-8841 [Abstract] [Full Text]  
  • Chiu, C. Y., Wu, E., Brown, S. L., Von Seggern, D. J., Nemerow, G. R., Stewart, P. L. (2001). Structural Analysis of a Fiber-Pseudotyped Adenovirus with Ocular Tropism Suggests Differential Modes of Cell Receptor Interactions. J. Virol. 75: 5375-5380 [Abstract] [Full Text]  
  • Arnberg, N., Kidd, A. H., Edlund, K., Olfat, F., Wadell, G. (2000). Initial Interactions of Subgenus D Adenoviruses with A549 Cellular Receptors: Sialic Acid versus alpha v Integrins. J. Virol. 74: 7691-7693 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»