This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gigler, A.
Right arrow Articles by Modrow, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gigler, A.
Right arrow Articles by Modrow, S.

 Previous Article  |  Next Article 

Journal of Virology, March 1999, p. 1974-1979, Vol. 73, No. 3
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Generation of Neutralizing Human Monoclonal Antibodies against Parvovirus B19 Proteins

Andreas Gigler,1 Simone Dorsch,1 Andrea Hemauer,1 Constance Williams,2 Sonnie Kim,3 Neal S. Young,3 Susan Zolla-Pazner,2,4 Hans Wolf,1 Miroslaw K. Gorny,2 and Susanne Modrow1,*

Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, 93053 Regensburg, Germany1; Department of Pathology, New York University Medical Center, New York, New York 100162; Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 208923; and Veterans Affairs Medical Center, New York, New York 100104

Received 10 September 1998/Accepted 9 December 1998

Infections caused by human parvovirus B19 are known to be controlled mainly by neutralizing antibodies. To analyze the immune reaction against parvovirus B19 proteins, four cell lines secreting human immunoglobulin G monoclonal antibodies (MAbs) were generated from two healthy donors and one human immunodeficiency virus type 1-seropositive individual with high serum titers against parvovirus. One MAb is specific for nonstructural protein NS1 (MAb 1424), two MAbs are specific for the unique region of minor capsid protein VP1 (MAbs 1418-1 and 1418-16), and one MAb is directed to major capsid protein VP2 (MAb 860-55D). Two MAbs, 1418-1 and 1418-16, which were generated from the same individual have identity in the cDNA sequences encoding the variable domains, with the exception of four base pairs resulting in only one amino acid change in the light chain. The NS1- and VP1-specific MAbs interact with linear epitopes, whereas the recognized epitope in VP2 is conformational. The MAbs specific for the structural proteins display strong virus-neutralizing activity. The VP1- and VP2-specific MAbs have the capacity to neutralize 50% of infectious parvovirus B19 in vitro at 0.08 and 0.73 µg/ml, respectively, demonstrating the importance of such antibodies in the clearance of B19 viremia. The NS1-specific MAb mediated weak neutralizing activity and required 47.7 µg/ml for 50% neutralization. The human MAbs with potent neutralizing activity could be used for immunotherapy of chronically B19 virus-infected individuals and acutely infected pregnant women. Furthermore, the knowledge gained regarding epitopes which induce strongly neutralizing antibodies may be important for vaccine development.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauss-Allee 11, 95053 Regensburg, Germany. Phone: 49-941-944-6454. Fax: 49-941-944-6402. E-mail: susanne.modrow{at}klinik.uni-regensburg.de.

Journal of Virology, March 1999, p. 1974-1979, Vol. 73, No. 3
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Herse, F., Verlohren, S., Wenzel, K., Pape, J., Muller, D. N., Modrow, S., Wallukat, G., Luft, F. C., Redman, C. W.G., Dechend, R. (2009). Prevalence of Agonistic Autoantibodies Against the Angiotensin II Type 1 Receptor and Soluble fms-Like Tyrosine Kinase 1 in a Gestational Age-Matched Case Study. Hypertension 53: 393-398 [Abstract] [Full Text]  
  • Bonsch, C., Kempf, C., Ros, C. (2008). Interaction of Parvovirus B19 with Human Erythrocytes Alters Virus Structure and Cell Membrane Integrity. J. Virol. 82: 11784-11791 [Abstract] [Full Text]  
  • Ekman, A., Hokynar, K., Kakkola, L., Kantola, K., Hedman, L., Bonden, H., Gessner, M., Aberham, C., Norja, P., Miettinen, S., Hedman, K., Soderlund-Venermo, M. (2007). Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3. J. Virol. 81: 6927-6935 [Abstract] [Full Text]  
  • Ros, C., Gerber, M., Kempf, C. (2006). Conformational Changes in the VP1-Unique Region of Native Human Parvovirus B19 Lead to Exposure of Internal Sequences That Play a Role in Virus Neutralization and Infectivity. J. Virol. 80: 12017-12024 [Abstract] [Full Text]  
  • Lupescu, A., Bock, C.-T., Lang, P. A., Aberle, S., Kaiser, H., Kandolf, R., Lang, F. (2006). Phospholipase A2 Activity-Dependent Stimulation of Ca2+ Entry by Human Parvovirus B19 Capsid Protein VP1. J. Virol. 80: 11370-11380 [Abstract] [Full Text]  
  • Gorny, M. K., Williams, C., Volsky, B., Revesz, K., Wang, X.-H., Burda, S., Kimura, T., Konings, F. A. J., Nadas, A., Anyangwe, C. A., Nyambi, P., Krachmarov, C., Pinter, A., Zolla-Pazner, S. (2006). Cross-Clade Neutralizing Activity of Human Anti-V3 Monoclonal Antibodies Derived from the Cells of Individuals Infected with Non-B Clades of Human Immunodeficiency Virus Type 1. J. Virol. 80: 6865-6872 [Abstract] [Full Text]  
  • Gorny, M. K., Stamatatos, L., Volsky, B., Revesz, K., Williams, C., Wang, X.-H., Cohen, S., Staudinger, R., Zolla-Pazner, S. (2005). Identification of a New Quaternary Neutralizing Epitope on Human Immunodeficiency Virus Type 1 Virus Particles. J. Virol. 79: 5232-5237 [Abstract] [Full Text]  
  • Candotti, D., Etiz, N., Parsyan, A., Allain, J.-P. (2004). Identification and Characterization of Persistent Human Erythrovirus Infection in Blood Donor Samples. J. Virol. 78: 12169-12178 [Abstract] [Full Text]  
  • Takasawa, N., Munakata, Y., Ishii, K. K., Takahashi, Y., Takahashi, M., Fu, Y., Ishii, T., Fujii, H., Saito, T., Takano, H., Noda, T., Suzuki, M., Nose, M., Zolla-Patzner, S., Sasaki, T. (2004). Human Parvovirus B19 Transgenic Mice Become Susceptible to Polyarthritis. J. Immunol. 173: 4675-4683 [Abstract] [Full Text]  
  • Chien, P. C. Jr., Cohen, S., Tuen, M., Arthos, J., Chen, P.-d., Patel, S., Hioe, C. E. (2004). Human Immunodeficiency Virus Type 1 Evades T-Helper Responses by Exploiting Antibodies That Suppress Antigen Processing. J. Virol. 78: 7645-7652 [Abstract] [Full Text]  
  • Gorny, M. K., Revesz, K., Williams, C., Volsky, B., Louder, M. K., Anyangwe, C. A., Krachmarov, C., Kayman, S. C., Pinter, A., Nadas, A., Nyambi, P. N., Mascola, J. R., Zolla-Pazner, S. (2004). The V3 Loop Is Accessible on the Surface of Most Human Immunodeficiency Virus Type 1 Primary Isolates and Serves as a Neutralization Epitope. J. Virol. 78: 2394-2404 [Abstract] [Full Text]  
  • Kerr, J R, McCoy, M, Burke, B, Mattey, D L, Pravica, V, Hutchinson, I V (2003). Cytokine gene polymorphisms associated with symptomatic parvovirus B19 infection. J. Clin. Pathol. 56: 725-727 [Abstract] [Full Text]  
  • Bogdan, C. A., Alexander, A. A., Gorny, M. K., Matute, R., Marjanovic, N., Zolla-Pazner, S., Walden, P. D., Furneaux, H. M., Sidhu, G. S., Jacobson, D. R. (2003). Chronic Lymphocytic Leukemia with Prostate Infiltration Mediated by Specific Clonal Membrane-bound IgM. Cancer Res. 63: 2067-2071 [Abstract] [Full Text]  
  • Dembinski, J, Eis-Hubinger, A M, Maar, J, Schild, R, Bartmann, P (2003). Long term follow up of serostatus after maternofetal parvovirus B19 infection. Arch. Dis. Child. 88: 219-221 [Abstract] [Full Text]  
  • Lopez-Bueno, A., Mateu, M. G., Almendral, J. M. (2003). High Mutant Frequency in Populations of a DNA Virus Allows Evasion from Antibody Therapy in an Immunodeficient Host. J. Virol. 77: 2701-2708 [Abstract] [Full Text]  
  • Gorny, M. K., Williams, C., Volsky, B., Revesz, K., Cohen, S., Polonis, V. R., Honnen, W. J., Kayman, S. C., Krachmarov, C., Pinter, A., Zolla-Pazner, S. (2002). Human Monoclonal Antibodies Specific for Conformation-Sensitive Epitopes of V3 Neutralize Human Immunodeficiency Virus Type 1 Primary Isolates from Various Clades. J. Virol. 76: 9035-9045 [Abstract] [Full Text]  
  • Heegaard, E. D., Brown, K. E. (2002). Human Parvovirus B19. Clin. Microbiol. Rev. 15: 485-505 [Abstract] [Full Text]  
  • Dorsch, S., Liebisch, G., Kaufmann, B., von Landenberg, P., Hoffmann, J. H., Drobnik, W., Modrow, S. (2002). The VP1 Unique Region of Parvovirus B19 and Its Constituent Phospholipase A2-Like Activity. J. Virol. 76: 2014-2018 [Abstract] [Full Text]  
  • Harder, T. C., Hufnagel, M., Zahn, K., Beutel, K., Schmitt, H.-J., Ullmann, U., Rautenberg, P. (2001). New LightCycler PCR for Rapid and Sensitive Quantification of Parvovirus B19 DNA Guides Therapeutic Decision-Making in Relapsing Infections. J. Clin. Microbiol. 39: 4413-4419 [Abstract] [Full Text]  
  • Mbah, H. A., Burda, S., Gorny, M. K., Williams, C., Revesz, K., Zolla-Pazner, S., Nyambi, P. N. (2001). Effect of Soluble CD4 on Exposure of Epitopes on Primary, Intact, Native Human Immunodeficiency Virus Type 1 Virions of Different Genetic Clades. J. Virol. 75: 7785-7788 [Abstract] [Full Text]  
  • Raab, U., Bauer, B., Gigler, A., Beckenlehner, K., Wolf, H., Modrow, S. (2001). Cellular transcription factors that interact with p6 promoter elements of parvovirus B19. J. Gen. Virol. 82: 1473-1480 [Abstract] [Full Text]  
  • Dorsch, S., Kaufmann, B., Schaible, U., Prohaska, E., Wolf, H., Modrow, S. (2001). The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability. J. Gen. Virol. 82: 191-199 [Abstract] [Full Text]  
  • Nyambi, P. N., Nádas, A., Mbah, H. A., Burda, S., Williams, C., Gorny, M. K., Zolla-Pazner, S. (2000). Immunoreactivity of Intact Virions of Human Immunodeficiency Virus Type 1 (HIV-1) Reveals the Existence of Fewer HIV-1 Immunotypes than Genotypes. J. Virol. 74: 10670-10680 [Abstract] [Full Text]  
  • Keller, M. A., Stiehm, E. R. (2000). Passive Immunity in Prevention and Treatment of Infectious Diseases. Clin. Microbiol. Rev. 13: 602-614 [Abstract] [Full Text]  
  • Nyambi, P. N., Mbah, H. A., Burda, S., Williams, C., Gorny, M. K., Nádas, A., Zolla-Pazner, S. (2000). Conserved and Exposed Epitopes on Intact, Native, Primary Human Immunodeficiency Virus Type 1 Virions of Group M. J. Virol. 74: 7096-7107 [Abstract] [Full Text]  
  • Kerr, J. R., Cunniffe, V. S. (2000). Antibodies to parvovirus B19 non-structural protein are associated with chronic but not acute arthritis following B19 infection. Rheumatology (Oxford) 39: 903-908 [Abstract] [Full Text]  
  • Gorny, M. K., Zolla-Pazner, S. (2000). Recognition by Human Monoclonal Antibodies of Free and Complexed Peptides Representing the Prefusogenic and Fusogenic Forms of Human Immunodeficiency Virus Type 1 gp41. J. Virol. 74: 6186-6192 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»