This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cayabyab, M.
Right arrow Articles by Sodroski, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cayabyab, M.
Right arrow Articles by Sodroski, J. G.

 Previous Article  |  Next Article 

Journal of Virology, February 1999, p. 976-984, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Changes in Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Responsible for the Pathogenicity of a Multiply Passaged Simian-Human Immunodeficiency Virus (SHIV-HXBc2)

Mark Cayabyab,1,2 Gunilla B. Karlsson,1 Bijan A. Etemad-Moghadam,1 Wolfgang Hofmann,1 Tavis Steenbeke,3 Matilda Halloran,3 John W. Fanton,4 Michael K. Axthelm,4 Norman L. Letvin,3 and Joseph G. Sodroski1,2,*

Department of Cancer Immunology/AIDS, Dana-Farber Cancer Institute, Department of Pathology1 and Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center,3 Harvard Medical School, and Department of Immunology and Infectious Diseases, Harvard School of Public Health,2 Boston, Massachusetts 02115, and Oregon Regional Primate Research Center, Beaverton, Oregon 97006-34994

Received 14 July 1998/Accepted 14 October 1998

In vivo passage of a poorly replicating, nonpathogenic simian-human immunodeficiency virus (SHIV-HXBc2) generated an efficiently replicating virus, KU-1, that caused rapid CD4+ T-lymphocyte depletion and AIDS-like illness in monkeys (S. V. Joag, Z. Li, L. Foresman, E. B. Stephens, L.-J. Zhao, I. Adany, D. M. Pinson, H. M. McClure, and O. Narayan, J. Virol. 70:3189-3197, 1996). The env gene of the KU-1 virus was used to create a molecularly cloned virus, SHIV-HXBc2P 3.2, that differed from a nonpathogenic SHIV-HXBc2 virus in only 12 envelope glycoprotein residues. SHIV-HXBc2P 3.2 replicated efficiently and caused rapid and persistent CD4+ T-lymphocyte depletion in inoculated rhesus macaques. Compared with the envelope glycoproteins of the parental SHIV-HXBc2, the SHIV-HXBc2P 3.2 envelope glycoproteins supported more efficient infection of rhesus monkey peripheral blood mononuclear cells. Both the parental SHIV-HXBc2 and the pathogenic SHIV-HXBc2P 3.2 used CXCR4 but none of the other seven transmembrane segment receptors tested as a second receptor. Compared with the parental virus, viruses with the SHIV-HXBc2P 3.2 envelope glycoproteins were more resistant to neutralization by soluble CD4 and antibodies. Thus, changes in the envelope glycoproteins account for the ability of the passaged virus to deplete CD4+ T lymphocytes rapidly and specify increased replicative capacity and resistance to neutralization.

* Corresponding author. Mailing address: Department of Cancer Immunology/AIDS, Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney St., Boston, MA 02115. Phone: (617) 632-3371. Fax: (617) 632-4338. E-mail: joseph_sodroski{at}dfci.harvard.edu.

Journal of Virology, February 1999, p. 976-984, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Apetrei, C., Gautam, R., Sumpter, B., Carter, A. C., Gaufin, T., Staprans, S. I., Else, J., Barnes, M., Cao, R. Jr., Garg, S., Milush, J. M., Sodora, D. L., Pandrea, I., Silvestri, G. (2007). Virus Subtype-Specific Features of Natural Simian Immunodeficiency Virus SIVsmm Infection in Sooty Mangabeys. J. Virol. 81: 7913-7923 [Abstract] [Full Text]  
  • Kraft, Z., Derby, N. R., McCaffrey, R. A., Niec, R., Blay, W. M., Haigwood, N. L., Moysi, E., Saunders, C. J., Wrin, T., Petropoulos, C. J., McElrath, M. J., Stamatatos, L. (2007). Macaques Infected with a CCR5-Tropic Simian/Human Immunodeficiency Virus (SHIV) Develop Broadly Reactive Anti-HIV Neutralizing Antibodies. J. Virol. 81: 6402-6411 [Abstract] [Full Text]  
  • Dale, C. J., De Rose, R., Stratov, I., Chea, S., Montefiori, D. C., Thomson, S., Ramshaw, I. A., Coupar, B. E. H., Boyle, D. B., Law, M., Kent, S. J. (2004). Efficacy of DNA and Fowlpox Virus Priming/Boosting Vaccines for Simian/Human Immunodeficiency Virus. J. Virol. 78: 13819-13828 [Abstract] [Full Text]  
  • Sadjadpour, R., Theodore, T. S., Igarashi, T., Donau, O. K., Plishka, R. J., Buckler-White, A., Martin, M. A. (2004). Induction of Disease by a Molecularly Cloned Highly Pathogenic Simian Immunodeficiency Virus/Human Immunodeficiency Virus Chimera Is Multigenic. J. Virol. 78: 5513-5519 [Abstract] [Full Text]  
  • Payne, S. L., Pei, X.-f., Jia, B., Fagerness, A., Fuller, F. J. (2004). Influence of Long Terminal Repeat and Env on the Virulence Phenotype of Equine Infectious Anemia Virus. J. Virol. 78: 2478-2485 [Abstract] [Full Text]  
  • Peyerl, F. W., Barouch, D. H., Yeh, W. W., Bazick, H. S., Kunstman, J., Kunstman, K. J., Wolinsky, S. M., Letvin, N. L. (2003). Simian-Human Immunodeficiency Virus Escape from Cytotoxic T-Lymphocyte Recognition at a Structurally Constrained Epitope. J. Virol. 77: 12572-12578 [Abstract] [Full Text]  
  • Pistello, M., Matteucci, D., Giannecchini, S., Bonci, F., Sichi, O., Presciuttini, S., Bendinelli, M. (2003). Evolution of Two Amino Acid Positions Governing Broad Neutralization Resistance in a Strain of Feline Immunodeficiency Virus over 7 Years of Persistence in Cats. CVI 10: 1109-1116 [Abstract] [Full Text]  
  • Poumbourios, P., Maerz, A. L., Drummer, H. E. (2003). Functional Evolution of the HIV-1 Envelope Glycoprotein 120 Association Site of Glycoprotein 41. J. Biol. Chem. 278: 42149-42160 [Abstract] [Full Text]  
  • LaBonte, J. A., Madani, N., Sodroski, J. (2003). Cytolysis by CCR5-Using Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Is Dependent on Membrane Fusion and Can Be Inhibited by High Levels of CD4 Expression. J. Virol. 77: 6645-6659 [Abstract] [Full Text]  
  • Hsu, M., Harouse, J. M., Gettie, A., Buckner, C., Blanchard, J., Cheng-Mayer, C. (2002). Increased Mucosal Transmission but Not Enhanced Pathogenicity of the CCR5-Tropic, Simian AIDS-Inducing Simian/Human Immunodeficiency Virus SHIVSF162P3 Maps to Envelope gp120. J. Virol. 77: 989-998 [Abstract] [Full Text]  
  • Lue, J., Hsu, M., Yang, D., Marx, P., Chen, Z., Cheng-Mayer, C. (2002). Addition of a Single gp120 Glycan Confers Increased Binding to Dendritic Cell-Specific ICAM-3-Grabbing Nonintegrin and Neutralization Escape to Human Immunodeficiency Virus Type 1. J. Virol. 76: 10299-10306 [Abstract] [Full Text]  
  • Hofmann-Lehmann, R., Vlasak, J., Chenine, A.-L., Li, P.-L., Baba, T. W., Montefiori, D. C., McClure, H. M., Anderson, D. C., Ruprecht, R. M. (2002). Molecular Evolution of Human Immunodeficiency Virus env in Humans and Monkeys: Similar Patterns Occur during Natural Disease Progression or Rapid Virus Passage. J. Virol. 76: 5278-5284 [Abstract] [Full Text]  
  • Johnston, J. B., Power, C. (2002). Feline Immunodeficiency Virus Xenoinfection: the Role of Chemokine Receptors and Envelope Diversity. J. Virol. 76: 3626-3636 [Abstract] [Full Text]  
  • Chakrabarti, L. A., Ivanovic, T., Cheng-Mayer, C. (2002). Properties of the Surface Envelope Glycoprotein Associated with Virulence of Simian-Human Immunodeficiency Virus SHIVSF33A Molecular Clones. J. Virol. 76: 1588-1599 [Abstract] [Full Text]  
  • Giannecchini, S., Matteucci, D., Ferrari, A., Pistello, M., Bendinelli, M. (2001). Feline Immunodeficiency Virus-Infected Cat Sera Associated with the Development of Broad Neutralization Resistance In Vivo Drive Similar Reversions In Vitro. J. Virol. 75: 8868-8873 [Abstract] [Full Text]  
  • Nakajima, N., Lu, R., Engelman, A. (2001). Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines. J. Virol. 75: 7944-7955 [Abstract] [Full Text]  
  • Bendinelli, M., Pistello, M., Del Mauro, D., Cammarota, G., Maggi, F., Leonildi, A., Giannecchini, S., Bergamini, C., Matteucci, D. (2001). During Readaptation In Vivo, a Tissue Culture-Adapted Strain of Feline Immunodeficiency Virus Reverts to Broad Neutralization Resistance at Different Times in Individual Hosts but through Changes at the Same Position of the Surface Glycoprotein. J. Virol. 75: 4584-4593 [Abstract] [Full Text]  
  • Si, Z., Cayabyab, M., Sodroski, J. (2001). Envelope Glycoprotein Determinants of Neutralization Resistance in a Simian-Human Immunodeficiency Virus (SHIV-HXBc2P 3.2) Derived by Passage in Monkeys. J. Virol. 75: 4208-4218 [Abstract] [Full Text]  
  • Kolchinsky, P., Kiprilov, E., Bartley, P., Rubinstein, R., Sodroski, J. (2001). Loss of a Single N-Linked Glycan Allows CD4-Independent Human Immunodeficiency Virus Type 1 Infection by Altering the Position of the gp120 V1/V2 Variable Loops. J. Virol. 75: 3435-3443 [Abstract] [Full Text]  
  • Beaumont, T., van Nuenen, A., Broersen, S., Blattner, W. A., Lukashov, V. V., Schuitemaker, H. (2001). Reversal of Human Immunodeficiency Virus Type 1 IIIB to a Neutralization-Resistant Phenotype in an Accidentally Infected Laboratory Worker with a Progressive Clinical Course. J. Virol. 75: 2246-2252 [Abstract] [Full Text]  
  • Ye, Y., Si, Z. H., Moore, J. P., Sodroski, J. (2000). Association of Structural Changes in the V2 and V3 Loops of the gp120 Envelope Glycoprotein with Acquisition of Neutralization Resistance in a Simian-Human Immunodeficiency Virus Passaged In Vivo. J. Virol. 74: 11955-11962 [Abstract] [Full Text]  
  • Bannert, N., Schenten, D., Craig, S., Sodroski, J. (2000). The Level of CD4 Expression Limits Infection of Primary Rhesus Monkey Macrophages by a T-Tropic Simian Immunodeficiency Virus and Macrophagetropic Human Immunodeficiency Viruses. J. Virol. 74: 10984-10993 [Abstract] [Full Text]  
  • LaBonte, J. A., Patel, T., Hofmann, W., Sodroski, J. (2000). Importance of Membrane Fusion Mediated by Human Immunodeficiency Virus Envelope Glycoproteins for Lysis of Primary CD4-Positive T Cells. J. Virol. 74: 10690-10698 [Abstract] [Full Text]  
  • Beaumont, T., Broersen, S., van Nuenen, A., Huisman, H. G., de Roda Husman, A.-M., Heeney, J. L., Schuitemaker, H. (2000). Increased Neutralization Sensitivity and Reduced Replicative Capacity of Human Immunodeficiency Virus Type 1 after Short-Term In Vivo or In Vitro Passage through Chimpanzees. J. Virol. 74: 7699-7707 [Abstract] [Full Text]  
  • Chen, Z., Huang, Y., Zhao, X., Skulsky, E., Lin, D., Ip, J., Gettie, A., Ho, D. D. (2000). Enhanced Infectivity of an R5-Tropic Simian/Human Immunodeficiency Virus Carrying Human Immunodeficiency Virus Type 1 Subtype C Envelope after Serial Passages in Pig-Tailed Macaques (Macaca nemestrina). J. Virol. 74: 6501-6510 [Abstract] [Full Text]  
  • Kolchinsky, P., Mirzabekov, T., Farzan, M., Kiprilov, E., Cayabyab, M., Mooney, L. J., Choe, H., Sodroski, J. (1999). Adaptation of a CCR5-Using, Primary Human Immunodeficiency Virus Type 1 Isolate for CD4-Independent Replication. J. Virol. 73: 8120-8126 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»