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Journal of Virology, February 1999, p. 1734-1739, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Polyomavirus Large T Antigen Binds the Transcriptional Coactivator Protein p300

Maria Nemethova and Erhard Wintersberger*

Institut für Molekularbiologie der Universität Wien, Wiener Biozentrum, A-1030 Vienna, Austria

Received 22 June 1998/Accepted 23 October 1998

Using coimmunoprecipitation and glutathione S-transferase pulldown experiments, we found that polyomavirus large T antigen binds to p300 in vivo and in vitro. The N-terminal region of the viral protein, including the pRB binding motif, was dispensable for this interaction, which involved several regions within the C-terminal half of the large T antigen. Interestingly, anti-T antibody coimmunoprecipitated a subspecies of p300 which has high histone acetyltransferase activity.

* Corresponding author. Mailing address: Institut für Molekularbiologie der Universität Wien, Wiener Biozentrum, Dr. Bohrgasse 9, A-1030 Vienna, Austria. Phone: 43-1-4277-61704. Fax: 43-1-4277-61705. E-mail: Wi{at}Mol.Univie.Ac.At.

Journal of Virology, February 1999, p. 1734-1739, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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