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Journal of Virology, February 1999, p. 1624-1629, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Visualization of Protein-RNA Interactions in Cytoplasmic Polyhedrosis Virus

H. Zhang,1 J. Zhang,2 X. Yu,2 X. Lu,2 Q. Zhang,2 J. Jakana,3 D. H. Chen,1,4 X. Zhang,4 and Z. H. Zhou1,*

Department of Pathology and Laboratory Medicine, University of Texas---Houston Medical School,1 and Department of Biochemistry, Baylor College of Medicine,3 Houston, Texas, and State Key Lab for Biocontrol, Institute of Entomology, Zhongshan University, Guangzhou 510275,2 and Beijing Laboratory of Electron Microscopy, Institute of Physics, Chinese Academy of Sciences, Beijing 100080,4 China

Received 7 July 1998/Accepted 1 November 1998

Unlike the multiple-shelled organization of other Reoviridae members, cytoplasmic polyhedrosis virus (CPV) has a single-shelled capsid. The three-dimensional structures of full and empty CPV by electron cryomicroscopy show identical outer shells but differ inside. The outer surface reveals a T=1 icosahedral shell decorated with spikes at its icosahedral vertices. The internal space of the empty CPV is unoccupied except for 12 mushroom-shaped densities attributed to the transcriptional enzyme complexes. The ordered double-stranded RNA inside the full capsid forms spherical shells spaced 25 Å apart. The RNA-protein interactions suggest a mechanism for RNA transcription and release.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, University of Texas---Houston Medical School, Houston, TX 77030. Phone: (713) 500-5358. Fax: (713) 500-0730. E-mail: hong{at}casper.med.uth.tmc.edu.

Journal of Virology, February 1999, p. 1624-1629, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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