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Journal of Virology, December 1999, p. 9849-9857, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

T-Cell Vaccination Alters the Course of Murine Herpesvirus 68 Infection and the Establishment of Viral Latency in Mice

Luzheng Liu,1,2,dagger Edward J. Usherwood,1,Dagger Marcia A. Blackman,1,2,Dagger and David L. Woodland1,2,*

Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105,1 and Department of Pathology, University of Tennessee Medical Center, Memphis, Tennessee 381632

Received 26 May 1999/Accepted 15 July 1999

Diseases caused by gammaherpesviruses such as Epstein-Barr virus are a major health concern, and there is significant interest in developing vaccines against this class of viral infections. However, the requirements for effective control of gammaherpesvirus infection are only poorly understood. The recent development of the murine herpesvirus MHV-68 model provides an experimental tool to dissect the immune response to gammaherpesvirus infections. In this study, we investigated the impact of priming T cells specific for class I- and class II-restricted epitopes on the acute phase of the infection and the subsequent establishment of latency and infectious mononucleosis. The data show that vaccination with either major histocompatibility complex class I- or class II-restricted T-cell epitopes derived from lytic cycle proteins significantly reduced lung viral titers during the acute infection. Moreover, the peak level of latently infected spleen cells was significantly reduced following vaccination with immunodominant CD8+ T-cell epitopes. However, this vaccination approach did not prevent the long-term establishment of latency or the development of the infectious mononucleosis-like syndrome in infected mice. Thus, the virus is able to establish latency efficiently despite strong immunological control of the lytic infection.

* Corresponding author. Present address: Trudeau Institute, P.O. Box 59, Saranac Lake, NY 12983. Phone: (518) 891-3080. Fax: (518) 891-5126. E-mail: dwoodland{at}trudeauinstitute.org.

dagger Present address: Yerkes Regional Primate Center, Emory University, Atlanta, GA 30322.

Dagger Present address: Trudeau Institute, Saranac Lake, NY 12983.

Journal of Virology, December 1999, p. 9849-9857, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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