This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, S.
Right arrow Articles by Naif, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, S.
Right arrow Articles by Naif, H. M.

 Previous Article  |  Next Article 

Journal of Virology, December 1999, p. 9741-9755, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Persistent CCR5 Utilization and Enhanced Macrophage Tropism by Primary Blood Human Immunodeficiency Virus Type 1 Isolates from Advanced Stages of Disease and Comparison to Tissue-Derived Isolates

Shan Li,1 Julius Juarez,1 Mohammed Alali,1 Dominic Dwyer,2 Ronald Collman,3 Anthony Cunningham,1 and Hassan M. Naif1,*

Centre for Virus Research, Westmead Millennium Institute, National Centre for HIV Virology Research,1 Department of Virology, ICPMR,2 Westmead Hospital, University of Sydney, Westmead, NSW 2145, Australia, and University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-60603

Received 7 June 1999/Accepted 26 August 1999

Viral phenotype, tropism, coreceptor usage, and envelope gene diversity were examined in blood isolates collected from 27 individuals at different stages of human immunodeficiency virus type 1 (HIV-1) disease and tissue derived isolates from 10 individuals with AIDS. The majority (89%) of blood and all tissue HIV-1 isolates from all stages of infection were non-syncytium inducing and macrophage (M) tropic. Tropism and productive infection by HIV isolates in both monocytes and monocyte-derived macrophages (MDM) increased in advanced disease (HIV tropism for monocytes, 1 of 6 from categories I and II versus 11 of 21 [P = 0.05] from category IV and II [CD4 < 250]; and high-level replication in MDM, 1 of 6 from categories I and II versus 16 of 21 from categories IV and II [P = 0.015]). There was a high level of replication of blood and tissue isolates in T lymphocytes without restriction at any stage. Overall, the level of replication in MDM was 5- to 10-fold greater than in monocytes, with restriction in the latter occurring mainly at entry and later stages of replication. Only three blood isolates were identified as syncytium inducing, and all had a dualtropic phenotype. There was a significant increase of HIV envelope gene diversity, as shown by a heteroduplex mobility assay, in advanced disease; this may partly underlie the increase of HIV replication in MDM. Unlike blood isolates (even those from patients with advanced disease), tissue isolates displayed greater similarities (90%) in productive infection between MDM and monocytes. The majority (87%) of all isolates, including those from patients with advanced disease, used CCR5, and only 5 of 37 isolates showed expanded coreceptor usage. These results indicate that in the late stage of disease with increasing viral load and diversity, CCR5 utilization and M-tropism persist in blood and tissue and the replicative ability in macrophages increases. This suggests that these characteristics are advantageous to HIV and are important to disease progression.

* Corresponding author. Mailing address: Molecular Pathogenesis Laboratory, Centre for Virus Research, Westmead Millennium Institute, The University of Sydney, Westmead Hospital, NSW 2145, Australia. Phone: (61-2) 9845 6311. Fax: (61-2) 9624 6881. E-mail: Hassann{at}westgate.wh.usyd.edu.au.

Journal of Virology, December 1999, p. 9741-9755, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Johnston, Samantha. H., Lobritz, M. A., Nguyen, S., Lassen, K., Delair, S., Posta, F., Bryson, Y. J., Arts, E. J., Chou, T., Lee, B. (2009). A Quantitative Affinity-Profiling System That Reveals Distinct CD4/CCR5 Usage Patterns among Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus Strains. J. Virol. 83: 11016-11026 [Abstract] [Full Text]  
  • Etemad, B., Fellows, A., Kwambana, B., Kamat, A., Feng, Y., Lee, S., Sagar, M. (2009). Human Immunodeficiency Virus Type 1 V1-to-V5 Envelope Variants from the Chronic Phase of Infection Use CCR5 and Fuse More Efficiently than Those from Early after Infection. J. Virol. 83: 9694-9708 [Abstract] [Full Text]  
  • Giri, M. S., Nebozyhn, M., Raymond, A., Gekonge, B., Hancock, A., Creer, S., Nicols, C., Yousef, M., Foulkes, A. S., Mounzer, K., Shull, J., Silvestri, G., Kostman, J., Collman, R. G., Showe, L., Montaner, L. J. (2009). Circulating Monocytes in HIV-1-Infected Viremic Subjects Exhibit an Antiapoptosis Gene Signature and Virus- and Host-Mediated Apoptosis Resistance. J. Immunol. 182: 4459-4470 [Abstract] [Full Text]  
  • Baba, M., Miyake, H., Wang, X., Okamoto, M., Takashima, K. (2007). Isolation and Characterization of Human Immunodeficiency Virus Type 1 Resistant to the Small-Molecule CCR5 Antagonist TAK-652. Antimicrob. Agents Chemother. 51: 707-715 [Abstract] [Full Text]  
  • Goodenow, M. M., Collman, R. G. (2006). HIV-1 coreceptor preference is distinct from target cell tropism: a dual-parameter nomenclature to define viral phenotypes. J. Leukoc. Biol. 80: 965-972 [Abstract] [Full Text]  
  • Dunfee, R. L., Thomas, E. R., Gorry, P. R., Wang, J., Taylor, J., Kunstman, K., Wolinsky, S. M., Gabuzda, D. (2006). The HIV Env variant N283 enhances macrophage tropism and is associated with brain infection and dementia. Proc. Natl. Acad. Sci. USA 103: 15160-15165 [Abstract] [Full Text]  
  • Peters, P. J., Sullivan, W. M., Duenas-Decamp, M. J., Bhattacharya, J., Ankghuambom, C., Brown, R., Luzuriaga, K., Bell, J., Simmonds, P., Ball, J., Clapham, P. R. (2006). Non-macrophage-tropic human immunodeficiency virus type 1 r5 envelopes predominate in blood, lymph nodes, and semen: implications for transmission and pathogenesis.. J. Virol. 80: 6324-6332 [Abstract] [Full Text]  
  • Marchant, D., Neil, S. J. D., McKnight, A. (2006). Human immunodeficiency virus types 1 and 2 have different replication kinetics in human primary macrophage culture. J. Gen. Virol. 87: 411-418 [Abstract] [Full Text]  
  • Baba, M., Takashima, K., Miyake, H., Kanzaki, N., Teshima, K., Wang, X., Shiraishi, M., Iizawa, Y. (2005). TAK-652 Inhibits CCR5-Mediated Human Immunodeficiency Virus Type 1 Infection In Vitro and Has Favorable Pharmacokinetics in Humans. Antimicrob. Agents Chemother. 49: 4584-4591 [Abstract] [Full Text]  
  • Ghaffari, G., Tuttle, D. L., Briggs, D., Burkhardt, B. R., Bhatt, D., Andiman, W. A., Sleasman, J. W., Goodenow, M. M. (2005). Complex Determinants in Human Immunodeficiency Virus Type 1 Envelope gp120 Mediate CXCR4-Dependent Infection of Macrophages. J. Virol. 79: 13250-13261 [Abstract] [Full Text]  
  • Bobardt, M. D., Salmon, P., Wang, L., Esko, J. D., Gabuzda, D., Fiala, M., Trono, D., Van der Schueren, B., David, G., Gallay, P. A. (2004). Contribution of Proteoglycans to Human Immunodeficiency Virus Type 1 Brain Invasion. J. Virol. 78: 6567-6584 [Abstract] [Full Text]  
  • Chackerian, B., Briglio, L., Albert, P. S., Lowy, D. R., Schiller, J. T. (2004). Induction of Autoantibodies to CCR5 in Macaques and Subsequent Effects upon Challenge with an R5-Tropic Simian/Human Immunodeficiency Virus. J. Virol. 78: 4037-4047 [Abstract] [Full Text]  
  • Hanley, T. M., Kiefer, H. L. B., Schnitzler, A. C., Marcello, J. E., Viglianti, G. A. (2004). Retinoid-Dependent Restriction of Human Immunodeficiency Virus Type 1 Replication in Monocytes/Macrophages. J. Virol. 78: 2819-2830 [Abstract] [Full Text]  
  • Goodenow, M. M., Rose, S. L., Tuttle, D. L., Sleasman, J. W. (2003). HIV-1 fitness and macrophages. J. Leukoc. Biol. 74: 657-666 [Abstract] [Full Text]  
  • Komuro, I., Yokota, Y., Yasuda, S., Iwamoto, A., Kagawa, K. S. (2003). CSF-induced and HIV-1-mediated Distinct Regulation of Hck and C/EBP{beta} Represent a Heterogeneous Susceptibility of Monocyte-derived Macrophages to M-tropic HIV-1 Infection. JEM 198: 443-453 [Abstract] [Full Text]  
  • Garaci, E., Aquaro, S., Lapenta, C., Amendola, A., Spada, M., Covaceuszach, S., Perno, C.-F., Belardelli, F. (2003). Anti-nerve growth factor Ab abrogates macrophage-mediated HIV-1 infection and depletion of CD4+ T lymphocytes in hu-SCID mice. Proc. Natl. Acad. Sci. USA 100: 8927-8932 [Abstract] [Full Text]  
  • Reeves, J. D., Doms, R. W. (2002). Human immunodeficiency virus type 2. J. Gen. Virol. 83: 1253-1265 [Full Text]  
  • Gorry, P. R., Taylor, J., Holm, G. H., Mehle, A., Morgan, T., Cayabyab, M., Farzan, M., Wang, H., Bell, J. E., Kunstman, K., Moore, J. P., Wolinsky, S. M., Gabuzda, D. (2002). Increased CCR5 Affinity and Reduced CCR5/CD4 Dependence of a Neurovirulent Primary Human Immunodeficiency Virus Type 1 Isolate. J. Virol. 76: 6277-6292 [Abstract] [Full Text]  
  • Naif, H. M., Cunningham, A. L., Alali, M., Li, S., Nasr, N., Buhler, M. M., Schols, D., de Clercq, E., Stewart, G. (2002). A Human Immunodeficiency Virus Type 1 Isolate from an Infected Person Homozygous for CCR5{Delta}32 Exhibits Dual Tropism by Infecting Macrophages and MT2 Cells via CXCR4. J. Virol. 76: 3114-3124 [Abstract] [Full Text]  
  • Thompson, D. A. D., Cormier, E. G., Dragic, T. (2002). CCR5 and CXCR4 Usage by Non-Clade B Human Immunodeficiency Virus Type 1 Primary Isolates. J. Virol. 76: 3059-3064 [Abstract] [Full Text]  
  • Wang, T. H., Donaldson, Y. K., Brettle, R. P., Bell, J. E., Simmonds, P. (2001). Identification of Shared Populations of Human Immunodeficiency Virus Type 1 Infecting Microglia and Tissue Macrophages outside the Central Nervous System. J. Virol. 75: 11686-11699 [Abstract] [Full Text]  
  • Gorry, P. R., Bristol, G., Zack, J. A., Ritola, K., Swanstrom, R., Birch, C. J., Bell, J. E., Bannert, N., Crawford, K., Wang, H., Schols, D., De Clercq, E., Kunstman, K., Wolinsky, S. M., Gabuzda, D. (2001). Macrophage Tropism of Human Immunodeficiency Virus Type 1 Isolates from Brain and Lymphoid Tissues Predicts Neurotropism Independent of Coreceptor Specificity. J. Virol. 75: 10073-10089 [Abstract] [Full Text]  
  • Aquaro, S., Menten, P., Struyf, S., Proost, P., Van Damme, J., De Clercq, E., Schols, D. (2001). The LD78{beta} Isoform of MIP-1{alpha} Is the Most Potent CC-Chemokine in Inhibiting CCR5-Dependent Human Immunodeficiency Virus Type 1 Replication in Human Macrophages. J. Virol. 75: 4402-4406 [Abstract] [Full Text]  
  • Trkola, A., Ketas, T. J., Nagashima, K. A., Zhao, L., Cilliers, T., Morris, L., Moore, J. P., Maddon, P. J., Olson, W. C. (2001). Potent, Broad-Spectrum Inhibition of Human Immunodeficiency Virus Type 1 by the CCR5 Monoclonal Antibody PRO 140. J. Virol. 75: 579-588 [Abstract] [Full Text]  
  • Trouplin, V., Salvatori, F., Cappello, F., Obry, V., Brelot, A., Heveker, N., Alizon, M., Scarlatti, G., Clavel, F., Mammano, F. (2001). Determination of Coreceptor Usage of Human Immunodeficiency Virus Type 1 from Patient Plasma Samples by Using a Recombinant Phenotypic Assay. J. Virol. 75: 251-259 [Abstract] [Full Text]  
  • Wahl, S. M., Greenwell-Wild, T., Hale-Donze, H., Moutsopoulos, N., Orenstein, J. M. (2000). Permissive factors for HIV-1 infection of macrophages. J. Leukoc. Biol. 68: 303-310 [Abstract] [Full Text]  
  • Cunningham, A. L., Li, S, Juarez, J, Lynch, G, Alali, M., Naif, H. (2000). The level of HIV infection of macrophages is determined by interaction of viral and host cell genotypes. J. Leukoc. Biol. 68: 311-317 [Abstract] [Full Text]  
  • Zhang, Y.-j., Lou, B., Lal, R. B., Gettie, A., Marx, P. A., Moore, J. P. (2000). Use of Inhibitors To Evaluate Coreceptor Usage by Simian and Simian/Human Immunodeficiency Viruses and Human Immunodeficiency Virus Type 2 in Primary Cells. J. Virol. 74: 6893-6910 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»