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Journal of Virology, December 1999, p. 10514-10518, Vol. 73, No. 12
0022-538X/99/$04.00+0

Quantitation of Latent Varicella-Zoster Virus and Herpes Simplex Virus Genomes in Human Trigeminal Ganglia

Stephanie R. Pevenstein,1 Richard K. Williams,1 Daniel McChesney,1 Erik K. Mont,2 John E. Smialek,3 and Stephen E. Straus1,*

Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases,1 and Anatomical Pathology Service, Laboratory of Pathology, National Cancer Institute,2 National Institutes of Health, Bethesda, and Department of Pathology, University of Maryland School of Medicine, Baltimore,3 Maryland

Received 10 May 1999/Accepted 31 August 1999

Using real-time fluorescence PCR, we quantitated the numbers of copies of latent varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) genomes in 15 human trigeminal ganglia. Eight (53%) and 1 (7%) of 15 ganglia were PCR positive for HSV-1 or -2 glycoprotein G genes, with means of 2,902 ± 1,082 (standard error of the mean) or 109 genomes/105 cells, respectively. Eleven of 14 (79%) to 13 of 15 (87%) of the ganglia were PCR positive for VZV gene 29, 31, or 62. Pooling of the results for the three VZV genes yielded a mean of 258 ± 38 genomes/105 ganglion cells. These levels of latent viral genome loads have implications for virus distribution in and reactivation from human sensory ganglia.

* Corresponding author. Mailing address: LCI, NIAID, NIH, Building 10, 11N228, 10 Center Dr., Bethesda, MD 20892. Phone: (301) 496-5807. Fax: (301) 496-7383. E-mail: sstraus{at}nih.gov.

Journal of Virology, December 1999, p. 10514-10518, Vol. 73, No. 12
0022-538X/99/$04.00+0


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