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Journal of Virology, November 1999, p. 9063-9071, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Nasal Immunization of Mice with Human Papillomavirus Type 16 (HPV-16) Virus-Like Particles or with the HPV-16 L1 Gene Elicits Specific Cytotoxic T Lymphocytes in Vaginal Draining Lymph Nodes

Catherine Dupuy,1 Dominique Buzoni-Gatel,2 Antoine Touzé,1 Daniel Bout,2 and Pierre Coursaget1,*

Laboratoire de Virologie Moléculaire1 and Laboratoire Associé INRA d'Immunologie Parasitaire,2 EA2637 Processus Infectieux et Tumoraux, Faculté de Pharmacie, 37200 Tours, France

Received 19 April 1999/Accepted 16 July 1999

Human papillomavirus type 16 (HPV-16) infects the genital tract and is closely associated with the development of cervical cancer. HPV-16 initiates infection at the genital mucosal surface; thus, mucosal immune responses are likely to contribute to defense against HPV-16 infection. However, little information is available regarding the induction of immune responses in the genital tract mucosa. In this study, we evaluated the potential of intranasally administered papillomavirus vaccines to elicit both systemic and vaginal immune responses. HPV-16 virus-like particles (VLPs) produced by self-assembly of L1 protein and the HPV-16 L1 gene cloned into a mammalian expression vector were used as vaccines. Intranasally administered VLPs induced serum immunoglobulin G (IgG) and vaginal IgA secretory antibodies. Very weak serum IgG and vaginal IgA responses were found after DNA immunization. Both splenic and vaginal lymphocytes could be activated by intranasal immunization with VLPs and the HPV-16 L1 gene. Activated CD4+ Th1-like T cells were shown to synthesize gamma interferon, and activated CD8+ T cells were demonstrated to be cytotoxic.

* Corresponding author. Mailing address: Laboratoire des Processus Infectieux et Tumoraux, Faculté de Pharmacie, 31 Ave. Monge, 37200 Tours, France. Phone: (33) 02 47 36 72 56. Fax: (33) 02 47 36 71 88. E-mail: coursaget{at}univ-tours.fr.

Journal of Virology, November 1999, p. 9063-9071, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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