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Journal of Virology, October 1999, p. 8902-8906, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Epstein-Barr Virus-Encoded RK-BARF0 Protein
Expression
Norbert
Kienzle,*
Marion
Buck,
Sonia
Greco,
Kenia
Krauer, and
Tom B.
Sculley
EBV Unit, The Queensland Institute of Medical
Research and University of Queensland Joint Oncology Program, Brisbane,
Australia
Received 21 June 1999/Accepted 29 June 1999
The cellular localization of the Epstein-Barr virus-encoded
RK-BARF0 protein was analyzed by fluorescence microscopy and
immunoblotting. The recombinant RK-BARF0 protein was found to be
tightly bound to nuclear structures, whereas 16- to 20-kDa RK-BARF0
derivatives, generated by differential splicing of the RK-BARF0
transcript, were present throughout the cell. Moreover, a previously
generated anti-RK-BARF0 rabbit serum was found to cross-react with
cellular proteins, showing that the previously identified 30- to 35-kDa membrane-associated proteins do not represent RK-BARF0.
*
Corresponding author. Mailing address: Queensland
Institute of Medical Research, Post Office, Royal Brisbane Hospital,
Brisbane, QLD 4029, Australia. Phone: 61-7-33620349. Fax:
61-7-33620106. E-mail: norbertK{at}qimr.edu.au.
Journal of Virology, October 1999, p. 8902-8906, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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