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Journal of Virology, October 1999, p. 8403-8410, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Role of Dendritic Cells in the Immune Response Induced by Mouse Mammary Tumor Virus Superantigen

Frédéric Baribaud,1,* Ivan Maillard,1 Sonia Vacheron,2,3 Thomas Brocker,4 Heidi Diggelmann,1 and Hans Acha-Orbea2,3

Institute of Microbiology, University of Lausanne, CH-1011 Lausanne,1 Institute of Biochemistry, University of Lausanne, CH-1066 Epalinges,2 and Ludwig Institute for Cancer Research, Lausanne Branch,3 CH-1066 Epalinges, Switzerland, and Universität Freiburg, Medizinische Universitätsklinik, Abteilung Innere Medizin I, Medizinische Molekularbiologie, D-79106 Freiburg, Germany4

Received 4 March 1999/Accepted 9 July 1999

After mouse mammary tumor virus (MMTV) infection, B lymphocytes present a superantigen (Sag) and receive help from the unlimited number of CD4+ T cells expressing Sag-specific T-cell receptor Vbeta elements. The infected B cells divide and differentiate, similarly to what occurs in classical B-cell responses. The amplification of Sag-reactive T cells can be considered a primary immune response. Since B cells are usually not efficient in the activation of naive T cells, we addressed the question of whether professional antigen-presenting cells such as dendritic cells (DCs) are responsible for T-cell priming. We show here, using MMTV(SIM), a viral isolate which requires major histocompatibility complex class II I-E expression to induce a strong Sag response in vivo, that transgenic mice expressing I-E exclusively on DCs (I-Ealpha DC tg) reveal a strong Sag response. This Sag response was dependent on the presence of B cells, as indicated by the absence of stimulation in I-Ealpha DC tg mice lacking B cells (I-Ealpha DC tg µMT-/-), even if these B cells lack I-E expression. Furthermore, the involvement of either residual transgene expression by B cells or transfer of I-E from DCs to B cells was excluded by the use of mixed bone marrow chimeras. Our results indicate that after priming by DCs in the context of I-E, the MMTV(SIM) Sag can be recognized on the surface of B cells in the context of I-A. The most likely physiological relevance of the lowering of the antigen threshold required for T-cell/B-cell collaboration after DC priming is to allow B cells with a low affinity for antigen to receive T-cell help in a primary immune response.

* Corresponding author. Present address: Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, 806 Abramson, 34th and Civic Center Blvd., Philadelphia, PA 19104. Phone: (215) 573-7532. Fax: (215) 573-2883. E-mail: fbaribau{at}mail.med.upenn.edu.

Journal of Virology, October 1999, p. 8403-8410, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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