This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by López-Lastra, M.
Right arrow Articles by Darlix, J.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by López-Lastra, M.
Right arrow Articles by Darlix, J.-L.

 Previous Article  |  Next Article 

Journal of Virology, October 1999, p. 8393-8402, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of an Internal Ribosome Entry Segment in the 5' Region of the Mouse VL30 Retrotransposon and Its Use in the Development of Retroviral Vectors

Marcelo López-Lastra, Sandrine Ulrici, Caroline Gabus, and Jean-Luc Darlix*

Labo Rétro, Unité de Virologie Humaine-U412, Institut National de la Santé et de la Recherche Médicale, Ecole Normale Supérieure de Lyon, 69364 Lyon cedex 07, France

Received 1 February 1999/Accepted 12 July 1999

Mouse virus-like 30S RNAs (VL30m) constitute a family of retrotransposons, present at 100 to 200 copies, dispersed in the mouse genome. They display little sequence homology to Moloney murine leukemia virus (MoMLV), do not encode virus-like proteins, and have not been implicated in retroviral carcinogenesis. However, VL30 RNAs are efficiently packaged into MLV particles that are propagated in cell culture. In this study, we addressed whether the 5' region of VL30m could replace the 5' leader of MoMLV functionally in a recombinant vector construct. Our data confirm that the putative packaging sequence of VL30 is located within the 5' region (nucleotides 362 to 1149 with respect to the cap structure) and that it can replace the packaging sequence of MoMLV. We also show that VL30m contains an internal ribosome entry segment (IRES) in the 5' region, as do MoMLV, Friend murine leukemia virus, Harvey murine sarcoma virus, and avian reticuloendotheliosis virus type A. Our data show that both the packaging and IRES functions of the 5' region of VL30m RNA can be efficiently used to develop retrotransposon-based vectors.

* Corresponding author. Mailing address: Labo Rétro, Unité de Virologie Humaine-U412, Institut National de la Santé et de la Recherche Médicale, Ecole Normale Supérieure de Lyon, 69364 Lyon cedex 07, France. Phone: (33) 472 72 81 69. Fax: (33) 472 72 87 77. E-mail: Jean-Luc.Darlix{at}ens-lyon.fr.

Journal of Virology, October 1999, p. 8393-8402, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • RONFORT, C., DE BREYNE, S., SANDRIN, V., DARLIX, J.-L., OHLMANN, T. (2004). Characterization of two distinct RNA domains that regulate translation of the Drosophila gypsy retroelement. RNA 10: 504-515 [Abstract] [Full Text]  
  • Brasey, A., Lopez-Lastra, M., Ohlmann, T., Beerens, N., Berkhout, B., Darlix, J.-L., Sonenberg, N. (2003). The Leader of Human Immunodeficiency Virus Type 1 Genomic RNA Harbors an Internal Ribosome Entry Segment That Is Active during the G2/M Phase of the Cell Cycle. J. Virol. 77: 3939-3949 [Abstract] [Full Text]  
  • Buck, C. B., Shen, X., Egan, M. A., Pierson, T. C., Walker, C. M., Siliciano, R. F. (2001). The Human Immunodeficiency Virus Type 1 gag Gene Encodes an Internal Ribosome Entry Site. J. Virol. 75: 181-191 [Abstract] [Full Text]  
  • Ohlmann, T., Lopez-Lastra, M., Darlix, J.-L. (2000). An Internal Ribosome Entry Segment Promotes Translation of the Simian Immunodeficiency Virus Genomic RNA. J. Biol. Chem. 275: 11899-11906 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»