This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Casella, C. R.
Right arrow Articles by Finkel, T. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casella, C. R.
Right arrow Articles by Finkel, T. H.

 Previous Article  |  Next Article 

Journal of Virology, January 1999, p. 92-100, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Vpu Increases Susceptibility of Human Immunodeficiency Virus Type 1-Infected Cells to Fas Killing

Carolyn R. Casella,1 Eric L. Rapaport,1 and Terri H. Finkel1,2,*

Division of Basic Sciences, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206,1 and Departments of Immunology, Pediatrics, and Biochemistry & Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 802622

Received 2 July 1998/Accepted 25 September 1998

The importance of the Fas death pathway in human immunodeficiency virus (HIV) infection has been the subject of many studies. Missing from these studies is direct measurement of infected cell susceptibility to Fas-induced death. To address this question, we investigated whether T cells infected with HIV are more susceptible to Fas-induced death. We found that Fas cross-linking caused a decrease in the number of HIV-infected Jurkat T cells and CD4+ peripheral blood leukocytes (PBLs). We confirmed this finding by demonstrating that there were more apoptotic infected than uninfected cells after Fas ligation. The increase in sensitivity of HIV-infected cells to Fas killing mapped to vpu, while nef, vif, vpr, and second exon of tat did not appear to contribute. Furthermore, expression of Vpu in Jurkat T cells rendered them more susceptible to Fas-induced death. These results show that HIV-infected cells are more sensitive to Fas-induced death and that the Vpu protein of HIV contributes to this sensitivity. The increased sensitivity of HIV-infected cells to Fas-induced death might help explain why these cells have such a short in vivo half-life.

* Corresponding author. Mailing address: National Jewish Medical and Research Center, 1400 Jackson St., Denver, CO 80206. Phone: (303) 398-1408. Fax: (303) 398-1225. E-mail: finkelt{at}njc.org.

Journal of Virology, January 1999, p. 92-100, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Arokium, H., Kamata, M., Chen, I. (2009). Virion-Associated Vpr of Human Immunodeficiency Virus Type 1 Triggers Activation of Apoptotic Events and Enhances Fas-Induced Apoptosis in Human T Cells. J. Virol. 83: 11283-11297 [Abstract] [Full Text]  
  • Gutierrez-Sanmartin, D., Varela-Ledo, E., Aguilera, A., Romero-Yuste, S., Romero-Jung, P., Gomez-Tato, A., Regueiro, B. J. (2008). Implication of p38 mitogen-activated protein kinase isoforms ({alpha}, {beta}, {gamma} and {delta}) in CD4+ T-cell infection with human immunodeficiency virus type I. J. Gen. Virol. 89: 1661-1671 [Abstract] [Full Text]  
  • Chan, E. Y., Qian, W.-J., Diamond, D. L., Liu, T., Gritsenko, M. A., Monroe, M. E., Camp, D. G. II, Smith, R. D., Katze, M. G. (2007). Quantitative Analysis of Human Immunodeficiency Virus Type 1-Infected CD4+ Cell Proteome: Dysregulated Cell Cycle Progression and Nuclear Transport Coincide with Robust Virus Production. J. Virol. 81: 7571-7583 [Abstract] [Full Text]  
  • Iannello, A., Debbeche, O., Martin, E., Attalah, L. H., Samarani, S., Ahmad, A. (2006). Viral strategies for evading antiviral cellular immune responses of the host. J. Leukoc. Biol. 79: 16-35 [Abstract] [Full Text]  
  • Gulow, K., Kaminski, M., Darvas, K., Suss, D., Li-Weber, M., Krammer, P. H. (2005). HIV-1 Trans-Activator of Transcription Substitutes for Oxidative Signaling in Activation-Induced T Cell Death. J. Immunol. 174: 5249-5260 [Abstract] [Full Text]  
  • Besnard-Guerin, C., Belaidouni, N., Lassot, I., Segeral, E., Jobart, A., Marchal, C., Benarous, R. (2004). HIV-1 Vpu Sequesters {beta}-Transducin Repeat-containing Protein ({beta}TrCP) in the Cytoplasm and Provokes the Accumulation of {beta}-Catenin and Other SCF{beta}TrCP Substrates. J. Biol. Chem. 279: 788-795 [Abstract] [Full Text]  
  • Komoto, S., Tsuji, S., Ibrahim, M. S., Li, Y.-G., Warachit, J., Taniguchi, K., Ikuta, K. (2003). The Vpu Protein of Human Immunodeficiency Virus Type 1 Plays a Protective Role against Virus-Induced Apoptosis in Primary CD4+ T Lymphocytes. J. Virol. 77: 10304-10313 [Abstract] [Full Text]  
  • Alimonti, J. B., Ball, T. B., Fowke, K. R. (2003). Mechanisms of CD4+ T lymphocyte cell death in human immunodeficiency virus infection and AIDS. J. Gen. Virol. 84: 1649-1661 [Abstract] [Full Text]  
  • Lenardo, M. J., Angleman, S. B., Bounkeua, V., Dimas, J., Duvall, M. G., Graubard, M. B., Hornung, F., Selkirk, M. C., Speirs, C. K., Trageser, C., Orenstein, J. O., Bolton, D. L. (2002). Cytopathic Killing of Peripheral Blood CD4+ T Lymphocytes by Human Immunodeficiency Virus Type 1 Appears Necrotic rather than Apoptotic and Does Not Require env. J. Virol. 76: 5082-5093 [Abstract] [Full Text]  
  • Moise, A. R., Grant, J. R., Vitalis, T. Z., Jefferies, W. A. (2002). Adenovirus E3-6.7K Maintains Calcium Homeostasis and Prevents Apoptosis and Arachidonic Acid Release. J. Virol. 76: 1578-1587 [Abstract] [Full Text]  
  • Akari, H., Bour, S., Kao, S., Adachi, A., Strebel, K. (2001). The Human Immunodeficiency Virus Type 1 Accessory Protein Vpu Induces Apoptosis by Suppressing the Nuclear Factor {kappa}B-dependent Expression of Antiapoptotic Factors. JEM 194: 1299-1312 [Abstract] [Full Text]  
  • Mahlknecht, U., Deng, C., Lu, M. C., Greenough, T. C., Sullivan, J. L., O'Brien, W. A., Herbein, G. (2000). Resistance to Apoptosis in HIV-Infected CD4+ T Lymphocytes Is Mediated by Macrophages: Role for Nef and Immune Activation in Viral Persistence. J. Immunol. 165: 6437-6446 [Abstract] [Full Text]  
  • Nishizawa, M., Kamata, M., Katsumata, R., Aida, Y. (2000). A Carboxy-Terminally Truncated Form of the Human Immunodeficiency Virus Type 1 Vpr Protein Induces Apoptosis via G1 Cell Cycle Arrest. J. Virol. 74: 6058-6067 [Abstract] [Full Text]  
  • Micoli, K. J., Pan, G., Wu, Y., Williams, J. P., Cook, W. J., McDonald, J. M. (2000). Requirement of Calmodulin Binding by HIV-1 gp160 for Enhanced FAS-mediated Apoptosis. J. Biol. Chem. 275: 1233-1240 [Abstract] [Full Text]  
  • Hamm, T. E., Rekosh, D., Hammarskjöld, M.-L. (1999). Selection and Characterization of Human Immunodeficiency Virus Type 1 Mutants That Are Resistant to Inhibition by the Transdominant Negative RevM10 Protein. J. Virol. 73: 5741-5747 [Abstract] [Full Text]  
  • Bour, S., Perrin, C., Akari, H., Strebel, K. (2001). The Human Immunodeficiency Virus Type 1 Vpu Protein Inhibits NF-kappa B Activation by Interfering with beta TrCP-mediated Degradation of Ikappa B. J. Biol. Chem. 276: 15920-15928 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»