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Journal of Virology, January 1999, p. 760-766, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Fine Structure and Morphogenesis of Borna Disease Virus

Takehiro Kohno,1 Toshiyuki Goto,1,* Tomohiko Takasaki,2,dagger Chizuko Morita,1 Takaaki Nakaya,3 Kazuyoshi Ikuta,3 Ichiro Kurane,4 Kouichi Sano,1 and Masuyo Nakai5

Department of Microbiology,1 Osaka Medical College,5 Takatsuki, Osaka 569-8686, Department of Microbiology, Kinki University School of Medicine, Osaka-sayama, Osaka 589-8511,2 Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-0815,3 and Department of Virology I, National Institute of Infectious Disease, Toyama, Shinjuku-ku, Tokyo 162-8640,4 Japan

Received 11 May 1998/Accepted 16 September 1998

Borna disease virus (BDV), a negative nonsegmented single-stranded RNA virus, has not been fully characterized morphologically. Here we present what is to our knowledge the first data on the fine ultrastructure and morphogenesis of BDV. The supernatant of MDCK cells persistently infected with BDV treated with n-butyrate contained many virus-like particles and more BDV-specific RNA than that of untreated samples. The particles were spherical, enveloped, and approximately 130 nm in diameter; had spikes 7 nm in length; and reacted with BDV p40 antibody. A thin nucleocapsid, 4 nm in width, was present peripherally in contrast to the thick nucleocapsid of hemagglutinating virus of Japan. The BDV particles reproduced by budding on the cell surface.

* Corresponding author. Mailing address: Department of Microbiology, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki, Osaka 569-8686, Japan. Phone: 81-726-83-1221, ext. 2647. Fax: 81-726-84-6517. E-mail: tgoto{at}art.osaka-med.ac.jp.

dagger Present address: Department of Virology I, National Institute of Infectious Disease, Tokyo, Japan.

Journal of Virology, January 1999, p. 760-766, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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