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Journal of Virology, January 1999, p. 325-333, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Trafficking to the Plasma Membrane of the Seven-Transmembrane Protein Encoded by Human Herpesvirus 6 U51 Gene Involves a Cell-Specific Function Present in T Lymphocytes

Laura Menotti,1 Prisco Mirandola,1 Massimo Locati,2,3 and Gabriella Campadelli-Fiume1,*

Section on Microbiology and Virology, Department of Experimental Pathology, University of Bologna, Bologna,1 Institute of Pharmacological Research "M. Negri," Milan,2 and Section on General Pathology, Department of Biotechnology, University of Brescia, Brescia,3 Italy

Received 3 August 1998/Accepted 15 October 1998

The sequence of human herpesvirus 6 (HHV-6) U51 open reading frame predicts a protein of 301 amino acid residues with seven transmembrane domains. To identify and characterize U51, we derived antipeptide polyclonal antibodies and developed a transient expression assay. We ascertained that U51 was synthesized in cord blood mononuclear cells infected with either variant A- or variant B-HHV-6 and was transported to the surface of productively infected cells. When synthesized in transient expression systems, U51 intracellular trafficking was regulated in a cell-type-dependent fashion. In human monolayer HEK-293 and 143tk- cells, U51 accumulated predominantly in the endoplasmic reticulum and failed to be transported to the cell surface. In contrast, in T-lymphocytic cell lines J-Jhan, Molt-3, and Jurkat, U51 was successfully transported to the plasma membrane. We infer that transport of U51 to the cell surface requires a cell-specific function present in activated T lymphocytes and T-cell lines.

* Corresponding author. Mailing address: Dipartimento di Patologia Sperimentale, Sezione di Microbiologia e Virologia, Via San Giacomo, 12, 40126 Bologna, Italy. Phone: 39 051 354733/34. Fax: 39 051 354747. E-mail: campadel{at}kaiser.alma.unibo.it.

Journal of Virology, January 1999, p. 325-333, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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