This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buchholz, U. J.
Right arrow Articles by Conzelmann, K.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buchholz, U. J.
Right arrow Articles by Conzelmann, K.-K.

 Previous Article  |  Next Article 

Journal of Virology, January 1999, p. 251-259, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Generation of Bovine Respiratory Syncytial Virus (BRSV) from cDNA: BRSV NS2 Is Not Essential for Virus Replication in Tissue Culture, and the Human RSV Leader Region Acts as a Functional BRSV Genome Promoter

Ursula J. Buchholz,dagger Stefan Finke, and Karl-Klaus Conzelmann*

Department of Clinical Virology, Federal Research Center for Virus Diseases of Animals, D-72076 Tübingen, Germany

Received 2 July 1998/Accepted 8 October 1998

In order to generate recombinant bovine respiratory syncytial virus (BRSV), the genome of BRSV strain A51908, variant ATue51908, was cloned as cDNA. We provide here the sequence of the BRSV genome ends and of the entire L gene. This completes the sequence of the BRSV genome, which comprises a total of 15,140 nucleotides. To establish a vaccinia virus-free recovery system, a BHK-derived cell line stably expressing T7 RNA polymerase was generated (BSR T7/5). Recombinant BRSV was reproducibly recovered from cDNA constructs after T7 RNA polymerase-driven expression of antigenome sense RNA and of BRSV N, P, M2, and L proteins from transfected plasmids. Chimeric viruses in which the BRSV leader region was replaced by the human respiratory syncytial virus (HRSV) leader region replicated in cell culture as efficiently as their nonchimeric counterparts, demonstrating that all cis-acting sequences of the HRSV promoter are faithfully recognized by the BRSV polymerase complex. In addition, we report the successful recovery of a BRSV mutant lacking the complete NS2 gene, which encodes a nonstructural protein of unknown function. The NS2-deficient BRSV replicated autonomously and could be passaged, demonstrating that NS2 is not essential for virus replication in cell culture. However, growth of the mutant was considerably slower than and final infectious titers were reduced by a factor of at least 10 compared to wild-type BRSV, indicating that NS2 provides a supporting factor required for full replication capacity.

* Corresponding author. Mailing address: Federal Research Center for Virus Diseases of Animals, Paul-Ehrlich-Str. 28, D-72076 Tübingen, Germany. Phone: 49 7071 967205. Fax: 49 7071 967303. E-mail: conzelmann{at}tue.bfav.de.

dagger Present address: Department of Molecular and Cellular Virology, Federal Research Center for Virus Diseases of Animals, D-17498 Insel Riems, Germany.

Journal of Virology, January 1999, p. 251-259, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Drahos, J., Racaniello, V. R. (2009). Cleavage of IPS-1 in Cells Infected with Human Rhinovirus. J. Virol. 83: 11581-11587 [Abstract] [Full Text]  
  • Connolly, S. A., Leser, G. P., Jardetzky, T. S., Lamb, R. A. (2009). Bimolecular Complementation of Paramyxovirus Fusion and Hemagglutinin-Neuraminidase Proteins Enhances Fusion: Implications for the Mechanism of Fusion Triggering. J. Virol. 83: 10857-10868 [Abstract] [Full Text]  
  • Eifan, S. A., Elliott, R. M. (2009). Mutational Analysis of the Bunyamwera Orthobunyavirus Nucleocapsid Protein Gene. J. Virol. 83: 11307-11317 [Abstract] [Full Text]  
  • Paal, T., Brindley, M. A., St. Clair, C., Prussia, A., Gaus, D., Krumm, S. A., Snyder, J. P., Plemper, R. K. (2009). Probing the Spatial Organization of Measles Virus Fusion Complexes. J. Virol. 83: 10480-10493 [Abstract] [Full Text]  
  • Shi, X., Goli, J., Clark, G., Brauburger, K., Elliott, R. M. (2009). Functional analysis of the Bunyamwera orthobunyavirus Gc glycoprotein. J. Gen. Virol. 90: 2483-2492 [Abstract] [Full Text]  
  • Yoon, J.-J., Krumm, S. A., Ndungu, J. M., Hoffman, V., Bankamp, B., Rota, P. A., Sun, A., Snyder, J. P., Plemper, R. K. (2009). Target Analysis of the Experimental Measles Therapeutic AS-136A. Antimicrob. Agents Chemother. 53: 3860-3870 [Abstract] [Full Text]  
  • Ciancanelli, M. J., Volchkova, V. A., Shaw, M. L., Volchkov, V. E., Basler, C. F. (2009). Nipah Virus Sequesters Inactive STAT1 in the Nucleus via a P Gene-Encoded Mechanism. J. Virol. 83: 7828-7841 [Abstract] [Full Text]  
  • Malik, T. H., Wolbert, C., Nerret, L., Sauder, C., Rubin, S. (2009). Single amino acid changes in the mumps virus haemagglutinin-neuraminidase and polymerase proteins are associated with neuroattenuation. J. Gen. Virol. 90: 1741-1747 [Abstract] [Full Text]  
  • Baliji, S., Cammer, S. A., Sobral, B., Baker, S. C. (2009). Detection of Nonstructural Protein 6 in Murine Coronavirus-Infected Cells and Analysis of the Transmembrane Topology by Using Bioinformatics and Molecular Approaches. J. Virol. 83: 6957-6962 [Abstract] [Full Text]  
  • Tran, T.-L., Castagne, N., Dubosclard, V., Noinville, S., Koch, E., Moudjou, M., Henry, C., Bernard, J., Yeo, R. P., Eleouet, J.-F. (2009). The Respiratory Syncytial Virus M2-1 Protein Forms Tetramers and Interacts with RNA and P in a Competitive Manner. J. Virol. 83: 6363-6374 [Abstract] [Full Text]  
  • Fan, J., Son, K.-N., Arslan, S. Y., Liang, Z., Lipton, H. L. (2009). Theiler's Murine Encephalomyelitis Virus Leader Protein Is the Only Nonstructural Protein Tested That Induces Apoptosis When Transfected into Mammalian Cells. J. Virol. 83: 6546-6553 [Abstract] [Full Text]  
  • Mohamed, M. R., Rahman, M. M., Lanchbury, J. S., Shattuck, D., Neff, C., Dufford, M., van Buuren, N., Fagan, K., Barry, M., Smith, S., Damon, I., McFadden, G. (2009). Proteomic screening of variola virus reveals a unique NF-{kappa}B inhibitor that is highly conserved among pathogenic orthopoxviruses. Proc. Natl. Acad. Sci. USA 106: 9045-9050 [Abstract] [Full Text]  
  • de Graaf, M., Schrauwen, E. J. A., Herfst, S., van Amerongen, G., Osterhaus, A. D. M. E., Fouchier, R. A. M. (2009). Fusion protein is the main determinant of metapneumovirus host tropism. J. Gen. Virol. 90: 1408-1416 [Abstract] [Full Text]  
  • Moore, M. L., Chi, M. H., Luongo, C., Lukacs, N. W., Polosukhin, V. V., Huckabee, M. M., Newcomb, D. C., Buchholz, U. J., Crowe, J. E. Jr., Goleniewska, K., Williams, J. V., Collins, P. L., Peebles, R. S. Jr. (2009). A Chimeric A2 Strain of Respiratory Syncytial Virus (RSV) with the Fusion Protein of RSV Strain Line 19 Exhibits Enhanced Viral Load, Mucus, and Airway Dysfunction. J. Virol. 83: 4185-4194 [Abstract] [Full Text]  
  • Enterlein, S., Schmidt, K. M., Schumann, M., Conrad, D., Krahling, V., Olejnik, J., Muhlberger, E. (2009). The Marburg Virus 3' Noncoding Region Structurally and Functionally Differs from That of Ebola Virus. J. Virol. 83: 4508-4519 [Abstract] [Full Text]  
  • Reed, M. L., Yen, H.-L., DuBois, R. M., Bridges, O. A., Salomon, R., Webster, R. G., Russell, C. J. (2009). Amino Acid Residues in the Fusion Peptide Pocket Regulate the pH of Activation of the H5N1 Influenza Virus Hemagglutinin Protein. J. Virol. 83: 3568-3580 [Abstract] [Full Text]  
  • Ling, Z., Tran, K. C., Teng, M. N. (2009). Human Respiratory Syncytial Virus Nonstructural Protein NS2 Antagonizes the Activation of Beta Interferon Transcription by Interacting with RIG-I. J. Virol. 83: 3734-3742 [Abstract] [Full Text]  
  • Buchholz, U. J., Ward, J. M., Lamirande, E. W., Heinze, B., Krempl, C. D., Collins, P. L. (2009). Deletion of Nonstructural Proteins NS1 and NS2 from Pneumonia Virus of Mice Attenuates Viral Replication and Reduces Pulmonary Cytokine Expression and Disease. J. Virol. 83: 1969-1980 [Abstract] [Full Text]  
  • Murphy, A. M., Grdzelishvili, V. Z. (2009). Identification of Sendai Virus L Protein Amino Acid Residues Affecting Viral mRNA Cap Methylation. J. Virol. 83: 1669-1681 [Abstract] [Full Text]  
  • Marschalek, A., Finke, S., Schwemmle, M., Mayer, D., Heimrich, B., Stitz, L., Conzelmann, K.-K. (2009). Attenuation of Rabies Virus Replication and Virulence by Picornavirus Internal Ribosome Entry Site Elements. J. Virol. 83: 1911-1919 [Abstract] [Full Text]  
  • Shi, X., Elliott, R. M. (2009). Generation and analysis of recombinant Bunyamwera orthobunyaviruses expressing V5 epitope-tagged L proteins. J. Gen. Virol. 90: 297-306 [Abstract] [Full Text]  
  • Luthra, P., Sun, D., Wolfgang, M., He, B. (2008). AKT1-Dependent Activation of NF-{kappa}B by the L Protein of Parainfluenza Virus 5. J. Virol. 82: 10887-10895 [Abstract] [Full Text]  
  • Armer, H., Moffat, K., Wileman, T., Belsham, G. J., Jackson, T., Duprex, W. P., Ryan, M., Monaghan, P. (2008). Foot-and-Mouth Disease Virus, but Not Bovine Enterovirus, Targets the Host Cell Cytoskeleton via the Nonstructural Protein 3Cpro. J. Virol. 82: 10556-10566 [Abstract] [Full Text]  
  • Hass, M., Lelke, M., Busch, C., Becker-Ziaja, B., Gunther, S. (2008). Mutational Evidence for a Structural Model of the Lassa Virus RNA Polymerase Domain and Identification of Two Residues, Gly1394 and Asp1395, That Are Critical for Transcription but Not Replication of the Genome. J. Virol. 82: 10207-10217 [Abstract] [Full Text]  
  • Gainey, M. D., Manuse, M. J., Parks, G. D. (2008). A Hyperfusogenic F Protein Enhances the Oncolytic Potency of a Paramyxovirus Simian Virus 5 P/V Mutant without Compromising Sensitivity to Type I Interferon. J. Virol. 82: 9369-9380 [Abstract] [Full Text]  
  • Bartlett, E. J., Cruz, A.-M., Esker, J., Castano, A., Schomacker, H., Surman, S. R., Hennessey, M., Boonyaratanakornkit, J., Pickles, R. J., Collins, P. L., Murphy, B. R., Schmidt, A. C. (2008). Human Parainfluenza Virus Type 1 C Proteins Are Nonessential Proteins That Inhibit the Host Interferon and Apoptotic Responses and Are Required for Efficient Replication in Nonhuman Primates. J. Virol. 82: 8965-8977 [Abstract] [Full Text]  
  • Timani, K. A., Sun, D., Sun, M., Keim, C., Lin, Y., Schmitt, P. T., Schmitt, A. P., He, B. (2008). A Single Amino Acid Residue Change in the P Protein of Parainfluenza Virus 5 Elevates Viral Gene Expression. J. Virol. 82: 9123-9133 [Abstract] [Full Text]  
  • Habjan, M., Penski, N., Spiegel, M., Weber, F. (2008). T7 RNA polymerase-dependent and -independent systems for cDNA-based rescue of Rift Valley fever virus. J. Gen. Virol. 89: 2157-2166 [Abstract] [Full Text]  
  • Tsurudome, M., Nishio, M., Ito, M., Tanahashi, S., Kawano, M., Komada, H., Ito, Y. (2008). Effects of Hemagglutinin-Neuraminidase Protein Mutations on Cell-Cell Fusion Mediated by Human Parainfluenza Type 2 Virus. J. Virol. 82: 8283-8295 [Abstract] [Full Text]  
  • Hussain, S., Perlman, S., Gallagher, T. M. (2008). Severe Acute Respiratory Syndrome Coronavirus Protein 6 Accelerates Murine Hepatitis Virus Infections by More than One Mechanism. J. Virol. 82: 7212-7222 [Abstract] [Full Text]  
  • Nishio, M., Ohtsuka, J., Tsurudome, M., Nosaka, T., Kolakofsky, D. (2008). Human Parainfluenza Virus Type 2 V Protein Inhibits Genome Replication by Binding to the L Protein: Possible Role in Promoting Viral Fitness. J. Virol. 82: 6130-6138 [Abstract] [Full Text]  
  • Rawling, J., Garcia-Barreno, B., Melero, J. A. (2008). Insertion of the Two Cleavage Sites of the Respiratory Syncytial Virus Fusion Protein in Sendai Virus Fusion Protein Leads to Enhanced Cell-Cell Fusion and a Decreased Dependency on the HN Attachment Protein for Activity. J. Virol. 82: 5986-5998 [Abstract] [Full Text]  
  • Lee, J. K., Prussia, A., Paal, T., White, L. K., Snyder, J. P., Plemper, R. K. (2008). Functional Interaction between Paramyxovirus Fusion and Attachment Proteins. J. Biol. Chem. 283: 16561-16572 [Abstract] [Full Text]  
  • Sleeman, K., Bankamp, B., Hummel, K. B., Lo, M. K., Bellini, W. J., Rota, P. A. (2008). The C, V and W proteins of Nipah virus inhibit minigenome replication. J. Gen. Virol. 89: 1300-1308 [Abstract] [Full Text]  
  • Simmonds, P., Karakasiliotis, I., Bailey, D., Chaudhry, Y., Evans, D. J., Goodfellow, I. G. (2008). Bioinformatic and functional analysis of RNA secondary structure elements among different genera of human and animal caliciviruses. Nucleic Acids Res 36: 2530-2546 [Abstract] [Full Text]  
  • de Graaf, M., Herfst, S., Schrauwen, E. J. A., Choi, Y., van den Hoogen, B. G., Osterhaus, A. D. M. E., Fouchier, R. A. M. (2008). Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses. J. Gen. Virol. 89: 975-983 [Abstract] [Full Text]  
  • Pulmanausahakul, R., Li, J., Schnell, M. J., Dietzschold, B. (2008). The Glycoprotein and the Matrix Protein of Rabies Virus Affect Pathogenicity by Regulating Viral Replication and Facilitating Cell-to-Cell Spread. J. Virol. 82: 2330-2338 [Abstract] [Full Text]  
  • Ling, R., Sinkovic, S., Toquin, D., Guionie, O., Eterradossi, N., Easton, A. J. (2008). Deletion of the SH gene from avian metapneumovirus has a greater impact on virus production and immunogenicity in turkeys than deletion of the G gene or M2-2 open reading frame. J. Gen. Virol. 89: 525-533 [Abstract] [Full Text]  
  • Klingen, Y., Conzelmann, K.-K., Finke, S. (2008). Double-Labeled Rabies Virus: Live Tracking of Enveloped Virus Transport. J. Virol. 82: 237-245 [Abstract] [Full Text]  
  • Sun, M., Fuentes, S. M., Timani, K., Sun, D., Murphy, C., Lin, Y., August, A., Teng, M. N., He, B. (2008). Akt Plays a Critical Role in Replication of Nonsegmented Negative-Stranded RNA Viruses. J. Virol. 82: 105-114 [Abstract] [Full Text]  
  • Shi, X., Kohl, A., Li, P., Elliott, R. M. (2007). Role of the Cytoplasmic Tail Domains of Bunyamwera Orthobunyavirus Glycoproteins Gn and Gc in Virus Assembly and Morphogenesis. J. Virol. 81: 10151-10160 [Abstract] [Full Text]  
  • Malik, T., Wolbert, C., Mauldin, J., Sauder, C., Carbone, K. M., Rubin, S. A. (2007). Functional consequences of attenuating mutations in the haemagglutinin neuraminidase, fusion and polymerase proteins of a wild-type mumps virus strain. J. Gen. Virol. 88: 2533-2541 [Abstract] [Full Text]  
  • Krempl, C. D., Wnekowicz, A., Lamirande, E. W., Nayebagha, G., Collins, P. L., Buchholz, U. J. (2007). Identification of a Novel Virulence Factor in Recombinant Pneumonia Virus of Mice. J. Virol. 81: 9490-9501 [Abstract] [Full Text]  
  • Chaudhry, Y., Skinner, M. A., Goodfellow, I. G. (2007). Recovery of genetically defined murine norovirus in tissue culture by using a fowlpox virus expressing T7 RNA polymerase. J. Gen. Virol. 88: 2091-2100 [Abstract] [Full Text]  
  • Gardner, A. E., Dutch, R. E. (2007). A Conserved Region in the F2 Subunit of Paramyxovirus Fusion Proteins Is Involved In Fusion Regulation. J. Virol. 81: 8303-8314 [Abstract] [Full Text]  
  • White, L. K., Yoon, J.-J., Lee, J. K., Sun, A., Du, Y., Fu, H., Snyder, J. P., Plemper, R. K. (2007). Nonnucleoside Inhibitor of Measles Virus RNA-Dependent RNA Polymerase Complex Activity. Antimicrob. Agents Chemother. 51: 2293-2303 [Abstract] [Full Text]  
  • Kochs, G., Garcia-Sastre, A., Martinez-Sobrido, L. (2007). Multiple Anti-Interferon Actions of the Influenza A Virus NS1 Protein. J. Virol. 81: 7011-7021 [Abstract] [Full Text]  
  • Bukreyev, A., Rollin, P. E., Tate, M. K., Yang, L., Zaki, S. R., Shieh, W.-J., Murphy, B. R., Collins, P. L., Sanchez, A. (2007). Successful Topical Respiratory Tract Immunization of Primates against Ebola Virus. J. Virol. 81: 6379-6388 [Abstract] [Full Text]  
  • Naylor, C. J., Ling, R., Edworthy, N., Savage, C. E., Easton, A. J. (2007). Avian metapneumovirus SH gene end and G protein mutations influence the level of protection of live-vaccine candidates. J. Gen. Virol. 88: 1767-1775 [Abstract] [Full Text]  
  • Muller, S., Gunther, S. (2007). Broad-Spectrum Antiviral Activity of Small Interfering RNA Targeting the Conserved RNA Termini of Lassa Virus. Antimicrob. Agents Chemother. 51: 2215-2218 [Abstract] [Full Text]  
  • Schmid, S., Mayer, D., Schneider, U., Schwemmle, M. (2007). Functional Characterization of the Major and Minor Phosphorylation Sites of the P Protein of Borna Disease Virus. J. Virol. 81: 5497-5507 [Abstract] [Full Text]  
  • Fenner, B. J., Goh, W., Kwang, J. (2007). Dissection of Double-Stranded RNA Binding Protein B2 from Betanodavirus. J. Virol. 81: 5449-5459 [Abstract] [Full Text]  
  • Biacchesi, S., Murphy, B. R., Collins, P. L., Buchholz, U. J. (2007). Frequent Frameshift and Point Mutations in the SH Gene of Human Metapneumovirus Passaged In Vitro. J. Virol. 81: 6057-6067 [Abstract] [Full Text]  
  • Blakqori, G., Delhaye, S., Habjan, M., Blair, C. D., Sanchez-Vargas, I., Olson, K. E., Attarzadeh-Yazdi, G., Fragkoudis, R., Kohl, A., Kalinke, U., Weiss, S., Michiels, T., Staeheli, P., Weber, F. (2007). La Crosse Bunyavirus Nonstructural Protein NSs Serves To Suppress the Type I Interferon System of Mammalian Hosts. J. Virol. 81: 4991-4999 [Abstract] [Full Text]  
  • Cordey, S., Roux, L. (2007). Further characterization of a paramyxovirus transcription initiation signal: search for required nucleotides upstream and importance of the N phase context. J. Gen. Virol. 88: 1555-1564 [Abstract] [Full Text]  
  • Deplanche, M., Lemaire, M., Mirandette, C., Bonnet, M., Schelcher, F., Meyer, G. (2007). In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome. J. Gen. Virol. 88: 1260-1265 [Abstract] [Full Text]  
  • Luque, L. E., Russell, C. J. (2007). Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein. J. Virol. 81: 3130-3141 [Abstract] [Full Text]  
  • Chase, G., Mayer, D., Hildebrand, A., Frank, R., Hayashi, Y., Tomonaga, K., Schwemmle, M. (2007). Borna Disease Virus Matrix Protein Is an Integral Component of the Viral Ribonucleoprotein Complex That Does Not Interfere with Polymerase Activity. J. Virol. 81: 743-749 [Abstract] [Full Text]  
  • Kohl, W., Grone, A., Moennig, V., Herrler, G. (2007). Expression of the surface glycoprotein E2 of Bovine viral diarrhea virus by recombinant vesicular stomatitis virus. J. Gen. Virol. 88: 157-165 [Abstract] [Full Text]  
  • Bukreyev, A., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L. (2006). Nonsegmented negative-strand viruses as vaccine vectors.. J. Virol. 80: 10293-10306 [Full Text]  
  • Leonard, V. H. J., Kohl, A., Hart, T. J., Elliott, R. M. (2006). Interaction of Bunyamwera Orthobunyavirus NSs Protein with Mediator Protein MED8: a Mechanism for Inhibiting the Interferon Response. J. Virol. 80: 9667-9675 [Abstract] [Full Text]  
  • Shi, X., Kohl, A., Leonard, V. H. J., Li, P., McLees, A., Elliott, R. M. (2006). Requirement of the N-Terminal Region of Orthobunyavirus Nonstructural Protein NSm for Virus Assembly and Morphogenesis.. J. Virol. 80: 8089-8099 [Abstract] [Full Text]  
  • Fenner, B. J., Goh, W., Kwang, J. (2006). Sequestration and Protection of Double-Stranded RNA by the Betanodavirus B2 Protein. J. Virol. 80: 6822-6833 [Abstract] [Full Text]  
  • Martin, D., Calder, L. J., Garcia-Barreno, B., Skehel, J. J., Melero, J. A. (2006). Sequence elements of the fusion peptide of human respiratory syncytial virus fusion protein required for activity. J. Gen. Virol. 87: 1649-1658 [Abstract] [Full Text]  
  • Govindarajan, D., Buchholz, U. J., Samal, S. K. (2006). Recovery of Avian Metapneumovirus Subgroup C from cDNA: Cross-Recognition of Avian and Human Metapneumovirus Support Proteins.. J. Virol. 80: 5790-5797 [Abstract] [Full Text]  
  • Biacchesi, S., Pham, Q. N., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L., Buchholz, U. J. (2006). Modification of the Trypsin-Dependent Cleavage Activation Site of the Human Metapneumovirus Fusion Protein To Be Trypsin Independent Does Not Increase Replication or Spread in Rodents or Nonhuman Primates.. J. Virol. 80: 5798-5806 [Abstract] [Full Text]  
  • Li, J., Wang, J. T., Whelan, S. P. J. (2006). A unique strategy for mRNA cap methylation used by vesicular stomatitis virus. Proc. Natl. Acad. Sci. USA 103: 8493-8498 [Abstract] [Full Text]  
  • Veits, J., Wiesner, D., Fuchs, W., Hoffmann, B., Granzow, H., Starick, E., Mundt, E., Schirrmeier, H., Mebatsion, T., Mettenleiter, T. C., Romer-Oberdorfer, A. (2006). Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenza. Proc. Natl. Acad. Sci. USA 103: 8197-8202 [Abstract] [Full Text]  
  • Brzozka, K., Finke, S., Conzelmann, K.-K. (2006). Inhibition of Interferon Signaling by Rabies Virus Phosphoprotein P: Activation-Dependent Binding of STAT1 and STAT2. J. Virol. 80: 2675-2683 [Abstract] [Full Text]  
  • Cordey, S., Roux, L. (2006). Transcribing paramyxovirus RNA polymerase engages the template at its 3' extremity.. J. Gen. Virol. 87: 665-672 [Abstract] [Full Text]  
  • Bukreyev, A., Yang, L., Zaki, S. R., Shieh, W.-J., Rollin, P. E., Murphy, B. R., Collins, P. L., Sanchez, A. (2006). A Single Intranasal Inoculation with a Paramyxovirus-Vectored Vaccine Protects Guinea Pigs against a Lethal-Dose Ebola Virus Challenge. J. Virol. 80: 2267-2279 [Abstract] [Full Text]  
  • Wilson, R. L., Fuentes, S. M., Wang, P., Taddeo, E. C., Klatt, A., Henderson, A. J., He, B. (2006). Function of Small Hydrophobic Proteins of Paramyxovirus. J. Virol. 80: 1700-1709 [Abstract] [Full Text]  
  • Enterlein, S., Volchkov, V., Weik, M., Kolesnikova, L., Volchkova, V., Klenk, H.-D., Muhlberger, E. (2006). Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30. J. Virol. 80: 1038-1043 [Abstract] [Full Text]  
  • Fenner, B. J., Thiagarajan, R., Chua, H. K., Kwang, J. (2006). Betanodavirus B2 Is an RNA Interference Antagonist That Facilitates Intracellular Viral RNA Accumulation. J. Virol. 80: 85-94 [Abstract] [Full Text]  
  • Kohl, A., Lowen, A. C., Leonard, V. H. J., Elliott, R. M. (2006). Genetic elements regulating packaging of the Bunyamwera orthobunyavirus genome. J. Gen. Virol. 87: 177-187 [Abstract] [Full Text]  
  • Pham, Q. N., Biacchesi, S., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L., Buchholz, U. J. (2005). Chimeric Recombinant Human Metapneumoviruses with the Nucleoprotein or Phosphoprotein Open Reading Frame Replaced by That of Avian Metapneumovirus Exhibit Improved Growth In Vitro and Attenuation In Vivo. J. Virol. 79: 15114-15122 [Abstract] [Full Text]  
  • Edworthy, N. L., Easton, A. J. (2005). Mutational analysis of the avian pneumovirus conserved transcriptional gene start sequence identifying critical residues. J. Gen. Virol. 86: 3343-3347 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Ito, Y. (2005). Human Parainfluenza Virus Type 4 Is Incapable of Evading the Interferon-Induced Antiviral Effect. J. Virol. 79: 14756-14768 [Abstract] [Full Text]  
  • Cheng, X., Park, H., Zhou, H., Jin, H. (2005). Overexpression of the M2-2 Protein of Respiratory Syncytial Virus Inhibits Viral Replication. J. Virol. 79: 13943-13952 [Abstract] [Full Text]  
  • Shi, X., Brauburger, K., Elliott, R. M. (2005). Role of N-Linked Glycans on Bunyamwera Virus Glycoproteins in Intracellular Trafficking, Protein Folding, and Virus Infectivity. J. Virol. 79: 13725-13734 [Abstract] [Full Text]  
  • Meulendyke, K. A., Wurth, M. A., McCann, R. O., Dutch, R. E. (2005). Endocytosis Plays a Critical Role in Proteolytic Processing of the Hendra Virus Fusion Protein. J. Virol. 79: 12643-12649 [Abstract] [Full Text]  
  • Pager, C. T., Dutch, R. E. (2005). Cathepsin L Is Involved in Proteolytic Processing of the Hendra Virus Fusion Protein. J. Virol. 79: 12714-12720 [Abstract] [Full Text]  
  • Lowen, A. C., Elliott, R. M. (2005). Mutational Analyses of the Nonconserved Sequences in the Bunyamwera Orthobunyavirus S Segment Untranslated Regions. J. Virol. 79: 12861-12870 [Abstract] [Full Text]  
  • Leonard, V. H. J., Kohl, A., Osborne, J. C., McLees, A., Elliott, R. M. (2005). Homotypic Interaction of Bunyamwera Virus Nucleocapsid Protein. J. Virol. 79: 13166-13172 [Abstract] [Full Text]  
  • Blakqori, G., Weber, F. (2005). Efficient cDNA-Based Rescue of La Crosse Bunyaviruses Expressing or Lacking the Nonstructural Protein NSs. J. Virol. 79: 10420-10428 [Abstract] [Full Text]  
  • Weik, M., Enterlein, S., Schlenz, K., Muhlberger, E. (2005). The Ebola Virus Genomic Replication Promoter Is Bipartite and Follows the Rule of Six. J. Virol. 79: 10660-10671 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Garcin, D., Kolakofsky, D., Ito, Y. (2005). Identification of Paramyxovirus V Protein Residues Essential for STAT Protein Degradation and Promotion of Virus Replication. J. Virol. 79: 8591-8601 [Abstract] [Full Text]  
  • Brzozka, K., Finke, S., Conzelmann, K.-K. (2005). Identification of the Rabies Virus Alpha/Beta Interferon Antagonist: Phosphoprotein P Interferes with Phosphorylation of Interferon Regulatory Factor 3. J. Virol. 79: 7673-7681 [Abstract] [Full Text]  
  • Carter, J. R., Pager, C. T., Fowler, S. D., Dutch, R. E. (2005). Role of N-Linked Glycosylation of the Hendra Virus Fusion Protein. J. Virol. 79: 7922-7925 [Abstract] [Full Text]  
  • Buchholz, U. J., Biacchesi, S., Pham, Q. N., Tran, K. C., Yang, L., Luongo, C. L., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L. (2005). Deletion of M2 Gene Open Reading Frames 1 and 2 of Human Metapneumovirus: Effects on RNA Synthesis, Attenuation, and Immunogenicity. J. Virol. 79: 6588-6597 [Abstract] [Full Text]  
  • Lowen, A. C., Boyd, A., Fazakerley, J. K., Elliott, R. M. (2005). Attenuation of Bunyavirus Replication by Rearrangement of Viral Coding and Noncoding Sequences. J. Virol. 79: 6940-6946 [Abstract] [Full Text]  
  • Hanika, A., Larisch, B., Steinmann, E., Schwegmann-Wessels, C., Herrler, G., Zimmer, G. (2005). Use of influenza C virus glycoprotein HEF for generation of vesicular stomatitis virus pseudotypes. J. Gen. Virol. 86: 1455-1465 [Abstract] [Full Text]  
  • Vulliemoz, D., Cordey, S., Mottet-Osman, G., Roux, L. (2005). Nature of a paramyxovirus replication promoter influences a nearby transcription signal. J. Gen. Virol. 86: 171-180 [Abstract] [Full Text]  
  • Schneider, U., Blechschmidt, K., Schwemmle, M., Staeheli, P. (2004). Overlap of Interaction Domains Indicates a Central Role of the P Protein in Assembly and Regulation of the Borna Disease Virus Polymerase Complex. J. Biol. Chem. 279: 55290-55296 [Abstract] [Full Text]  
  • Hass, M., Golnitz, U., Muller, S., Becker-Ziaja, B., Gunther, S. (2004). Replicon System for Lassa Virus. J. Virol. 78: 13793-13803 [Abstract] [Full Text]  
  • Ruiz-Arguello, M. B., Martin, D., Wharton, S. A., Calder, L. J., Martin, S. R., Cano, O., Calero, M., Garcia-Barreno, B., Skehel, J. J., Melero, J. A. (2004). Thermostability of the human respiratory syncytial virus fusion protein before and after activation: implications for the membrane-fusion mechanism. J. Gen. Virol. 85: 3677-3687 [Abstract] [Full Text]  
  • Biacchesi, S., Skiadopoulos, M. H., Yang, L., Lamirande, E. W., Tran, K. C., Murphy, B. R., Collins, P. L., Buchholz, U. J. (2004). Recombinant Human Metapneumovirus Lacking the Small Hydrophobic SH and/or Attachment G Glycoprotein: Deletion of G Yields a Promising Vaccine Candidate. J. Virol. 78: 12877-12887 [Abstract] [Full Text]  
  • Finke, S., Brzozka, K., Conzelmann, K.-K. (2004). Tracking Fluorescence-Labeled Rabies Virus: Enhanced Green Fluorescent Protein-Tagged Phosphoprotein P Supports Virus Gene Expression and Formation of Infectious Particles. J. Virol. 78: 12333-12343 [Abstract] [Full Text]  
  • Kohl, A., Dunn, E. F., Lowen, A. C., Elliott, R. M. (2004). Complementarity, sequence and structural elements within the 3' and 5' non-coding regions of the Bunyamwera orthobunyavirus S segment determine promoter strength. J. Gen. Virol. 85: 3269-3278 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»