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Journal of Virology, September 1998, p. 7681-7684, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Classical Swine Fever Virus Leader Proteinase Npro Is Not Required for Viral Replication in Cell Culture

Jon-Duri Tratschin,* Christian Moser, Nicolas Ruggli,dagger and Martin A. Hofmann

Institute of Virology and Immunoprophylaxis, CH-3147 Mittelhäusern, Switzerland

Received 12 February 1998/Accepted 3 June 1998

The sequence encoding the viral leader proteinase Npro was replaced by the murine ubiquitin gene in a full-length cDNA clone of the classical swine fever virus (CSFV) strain Alfort/187. The recombinant virus vA187-Ubi showed growth characteristics similar to those of the parent vA187-1 virus. At two occasions cells infected with vA187-Ubi exhibited a cytopathic effect and were found to contain a subgenomic viral RNA. This RNA lacked the same viral genes as the subgenomic RNA which has been found in all cytopathogenic CSFV strains analyzed so far, but it maintained the ubiquitin sequence.

* Corresponding author. Mailing address: Institute of Virology and Immunoprophylaxis, CH-3147 Mittelhäusern, Switzerland. Phone: 41 31 848 92 11. Fax: 41 31 848 92 22. E-mail: jon-duri.tratschin{at}ivi.admin.ch.

dagger Present address: ZLB Central Laboratory, Blood Transfusion Service SRC, CH-3022 Bern, Switzerland.

Journal of Virology, September 1998, p. 7681-7684, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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