This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Borza, C. M.
Right arrow Articles by Hutt-Fletcher, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borza, C. M.
Right arrow Articles by Hutt-Fletcher, L. M.

 Previous Article  |  Next Article 

Journal of Virology, September 1998, p. 7577-7582, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Epstein-Barr Virus Recombinant Lacking Expression of Glycoprotein gp150 Infects B Cells Normally but Is Enhanced for Infection of Epithelial Cells

Corina M. Borza and Lindsey M. Hutt-Fletcher*

School of Biological Sciences, University of Missouri---Kansas City, Kansas City, Missouri 64110

Received 5 March 1998/Accepted 20 May 1998

Glycoprotein gp150 is a highly glycosylated protein encoded by the BDLF3 open reading frame of Epstein-Barr virus (EBV). It does not have a homolog in the alpha- and betaherpesviruses, and its function is not known. To determine whether the protein is essential for replication of EBV in vitro, a recombinant virus which lacked its expression was made. The recombinant virus had no defects in assembly, egress, binding, or infectivity for B cells or epithelial cells. Infection of epithelial cells was, however, enhanced. The glycoprotein was sensitive to digestion with a glycoprotease that digests sialomucins, but no adhesion to cells that express selectins that bind to sialomucin ligands could be detected.

* Corresponding author. Mailing address: School of Biological Sciences, University of Missouri---Kansas City, 5007 Rockhill Rd., Kansas City, MO 64110. Phone: (816) 235-2575. Fax: (816) 235-5595. E-mail: huttfletcher{at}cctr.umkc.edu.

Journal of Virology, September 1998, p. 7577-7582, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fogg, M. H., Carville, A., Cameron, J., Quink, C., Wang, F. (2005). Reduced Prevalence of Epstein-Barr Virus-Related Lymphocryptovirus Infection in Sera from a New World Primate. J. Virol. 79: 10069-10072 [Abstract] [Full Text]  
  • Moody, C. A., Scott, R. S., Amirghahari, N., Nathan, C.-A., Young, L. S., Dawson, C. W., Sixbey, J. W. (2005). Modulation of the Cell Growth Regulator mTOR by Epstein-Barr Virus-Encoded LMP2A. J. Virol. 79: 5499-5506 [Abstract] [Full Text]  
  • May, J. S., Walker, J., Colaco, S., Stevenson, P. G. (2005). The Murine Gammaherpesvirus 68 ORF27 Gene Product Contributes to Intercellular Viral Spread. J. Virol. 79: 5059-5068 [Abstract] [Full Text]  
  • May, J. S., Coleman, H. M., Boname, J. M., Stevenson, P. G. (2005). Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein. J. Gen. Virol. 86: 919-928 [Abstract] [Full Text]  
  • Spear, P. G., Longnecker, R. (2003). Herpesvirus Entry: an Update. J. Virol. 77: 10179-10185 [Full Text]  
  • Moody, C. A., Scott, R. S., Su, T., Sixbey, J. W. (2003). Length of Epstein-Barr Virus Termini as a Determinant of Epithelial Cell Clonal Emergence. J. Virol. 77: 8555-8561 [Abstract] [Full Text]  
  • Rivailler, P., Cho, Y.-g., Wang, F. (2002). Complete Genomic Sequence of an Epstein-Barr Virus-Related Herpesvirus Naturally Infecting a New World Primate: a Defining Point in the Evolution of Oncogenic Lymphocryptoviruses. J. Virol. 76: 12055-12068 [Abstract] [Full Text]  
  • Rivailler, P., Jiang, H., Cho, Y.-g., Quink, C., Wang, F. (2002). Complete Nucleotide Sequence of the Rhesus Lymphocryptovirus: Genetic Validation for an Epstein-Barr Virus Animal Model. J. Virol. 76: 421-426 [Abstract] [Full Text]  
  • Cho, Y.-G., Ramer, J., Rivailler, P., Quink, C., Garber, R. L., Beier, D. R., Wang, F. (2001). An Epstein-Barr-related herpesvirus from marmoset lymphomas. Proc. Natl. Acad. Sci. USA 98: 1224-1229 [Abstract] [Full Text]  
  • Lake, C. M., Hutt-Fletcher, L. M. (2000). Epstein-Barr Virus That Lacks Glycoprotein gN Is Impaired in Assembly and Infection. J. Virol. 74: 11162-11172 [Abstract] [Full Text]  
  • Delecluse, H-J, Hammerschmidt, W (2000). The genetic approach to the Epstein-Barr virus: from basic virology to gene therapy. Mol. Pathol. 53: 270-279 [Abstract] [Full Text]  
  • Westphal, E. M., Blackstock, W., Feng, W., Israel, B., Kenney, S. C. (2000). Activation of Lytic Epstein-Barr Virus (EBV) Infection by Radiation and Sodium Butyrate in Vitro and in Vivo: A Potential Method for Treating EBV-positive Malignancies. Cancer Res. 60: 5781-5788 [Abstract] [Full Text]  
  • Molesworth, S. J., Lake, C. M., Borza, C. M., Turk, S. M., Hutt-Fletcher, L. M. (2000). Epstein-Barr Virus gH Is Essential for Penetration of B Cells but Also Plays a Role in Attachment of Virus to Epithelial Cells. J. Virol. 74: 6324-6332 [Abstract] [Full Text]  
  • Ruf, I. K., Rhyne, P. W., Yang, H., Borza, C. M., Hutt-Fletcher, L. M., Cleveland, J. L., Sample, J. T. (1999). Epstein-Barr Virus Regulates c-MYC, Apoptosis, and Tumorigenicity in Burkitt Lymphoma. Mol. Cell. Biol. 19: 1651-1660 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»