This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koelle, D. M.
Right arrow Articles by Kwok, W. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koelle, D. M.
Right arrow Articles by Kwok, W. W.

 Previous Article  |  Next Article 

Journal of Virology, September 1998, p. 7476-7483, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Recognition of Herpes Simplex Virus Type 2 Tegument Proteins by CD4 T Cells Infiltrating Human Genital Herpes Lesions

David M. Koelle,1,2,3,* Jeannine M. Frank,2,3 Matthew L. Johnson,2 and William W. Kwok4

Department of Medicine1 and Department of Laboratory Medicine,2 University of Washington, Seattle, Washington 98105; Fred Hutchinson Cancer Research Center, Seattle, Washington 981043; and Virginia Mason Research Center, Seattle, Washington 981014

Received 16 March 1998/Accepted 19 May 1998

The local cellular immune response to herpes simplex virus (HSV) is important in the control of recurrent HSV infection. The antiviral functions of infiltrating CD4-bearing T cells may include cytotoxicity, inhibition of viral growth, lymphokine secretion, and support of humoral and CD8 responses. The antigens recognized by many HSV-specific CD4 T cells localizing to genital HSV-2 lesions are unknown. T cells recognizing antigens encoded within map units 0.67 to 0.73 of HSV DNA are frequently recovered from herpetic lesions. Expression cloning with this region of DNA now shows that tegument protein VP22 and the viral dUTPase, encoded by genes UL49 and UL50, respectively, are T-cell antigens. Separate epitopes in VP22 were defined for T-cell clones from each of three patients. Reactivity with the tegument protein encoded by UL21 was identified for an additional patient. Three new epitopes were identified in VP16, a tegument protein associated with VP22. Some tegument-specific CD4 T-cell clones exhibited cytotoxic activity against HSV-infected cells. These results suggest that herpes simplex tegument proteins are processed for antigen presentation in vivo and are possible candidate compounds for herpes simplex vaccines.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, Room D3-100, 1100 Fairview Ave. North, P.O. Box 19024, Seattle, WA 98109. Phone: (206) 667-6807. Fax: (206) 667-6707. E-mail: viralimm{at}u.washington.edu.

Journal of Virology, September 1998, p. 7476-7483, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Muller, W. J., Dong, L., Vilalta, A., Byrd, B., Wilhelm, K. M., McClurkan, C. L., Margalith, M., Liu, C., Kaslow, D., Sidney, J., Sette, A., Koelle, D. M. (2009). Herpes simplex virus type 2 tegument proteins contain subdominant T-cell epitopes detectable in BALB/c mice after DNA immunization and infection. J. Gen. Virol. 90: 1153-1163 [Abstract] [Full Text]  
  • Milikan, J. C. M., Baarsma, G. S., Kuijpers, R. W. A. M., Osterhaus, A. D. M. E., Verjans, G. M. G. M. (2009). Human Ocular-Derived Virus-Specific CD4+ T Cells Control Varicella Zoster Virus Replication in Human Retinal Pigment Epithelial Cells. IOVS 50: 743-751 [Abstract] [Full Text]  
  • Kim, M., Taylor, J., Sidney, J., Mikloska, Z., Bodsworth, N., Lagios, K., Dunckley, H., Byth-Wilson, K., Denis, M., Finlayson, R., Khanna, R., Sette, A., Cunningham, A. L. (2008). Immunodominant Epitopes in Herpes Simplex Virus Type 2 Glycoprotein D Are Recognized by CD4 Lymphocytes from Both HSV-1 and HSV-2 Seropositive Subjects. J. Immunol. 181: 6604-6615 [Abstract] [Full Text]  
  • Zhang, X., Castelli, F. A., Zhu, X., Wu, M., Maillere, B., BenMohamed, L. (2008). Gender-Dependent HLA-DR-Restricted Epitopes Identified from Herpes Simplex Virus Type 1 Glycoprotein D. CVI 15: 1436-1449 [Abstract] [Full Text]  
  • Chentoufi, A. A., Zhang, X., Lamberth, K., Dasgupta, G., Bettahi, I., Nguyen, A., Wu, M., Zhu, X., Mohebbi, A., Buus, S., Wechsler, S. L., Nesburn, A. B., BenMohamed, L. (2008). HLA-A*0201-Restricted CD8+ Cytotoxic T Lymphocyte Epitopes Identified from Herpes Simplex Virus Glycoprotein D. J. Immunol. 180: 426-437 [Abstract] [Full Text]  
  • Jing, L., Chong, T. M., Byrd, B., McClurkan, C. L., Huang, J., Story, B. T., Dunkley, K. M., Aldaz-Carroll, L., Eisenberg, R. J., Cohen, G. H., Kwok, W. W., Sette, A., Koelle, D. M. (2007). Dominance and Diversity in the Primary Human CD4 T Cell Response to Replication-Competent Vaccinia Virus. J. Immunol. 178: 6374-6386 [Abstract] [Full Text]  
  • Hosken, N., McGowan, P., Meier, A., Koelle, D. M., Sleath, P., Wagener, F., Elliott, M., Grabstein, K., Posavad, C., Corey, L. (2006). Diversity of the CD8+ T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes.. J. Virol. 80: 5509-5515 [Abstract] [Full Text]  
  • Eriksson, K., Bellner, L., Gorander, S., Lowhagen, G.-B., Tunback, P., Rydberg, K., Liljeqvist, J.-A. (2004). CD4+ T-cell responses to herpes simplex virus type 2 (HSV-2) glycoprotein G are type specific and differ in symptomatic and asymptomatic HSV-2-infected individuals. J. Gen. Virol. 85: 2139-2147 [Abstract] [Full Text]  
  • Verschoor, A., Brockman, M. A., Gadjeva, M., Knipe, D. M., Carroll, M. C. (2003). Myeloid C3 Determines Induction of Humoral Responses to Peripheral Herpes Simplex Virus Infection. J. Immunol. 171: 5363-5371 [Abstract] [Full Text]  
  • Koelle, D. M., Corey, L. (2003). Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research. Clin. Microbiol. Rev. 16: 96-113 [Abstract] [Full Text]  
  • Novak, E. J., Liu, A. W., Gebe, J. A., Falk, B. A., Nepom, G. T., Koelle, D. M., Kwok, W. W. (2001). Tetramer-Guided Epitope Mapping: Rapid Identification and Characterization of Immunodominant CD4+ T Cell Epitopes from Complex Antigens. J. Immunol. 166: 6665-6670 [Abstract] [Full Text]  
  • Koelle, D. M., Chen, H. B., Gavin, M. A., Wald, A., Kwok, W. W., Corey, L. (2001). CD8 CTL from Genital Herpes Simplex Lesions: Recognition of Viral Tegument and Immediate Early Proteins and Lysis of Infected Cutaneous Cells. J. Immunol. 166: 4049-4058 [Abstract] [Full Text]  
  • Koelle, D. M., Reymond, S. N., Chen, H., Kwok, W. W., McClurkan, C., Gyaltsong, T., Petersdorf, E. W., Rotkis, W., Talley, A. R., Harrison, D. A. (2000). Tegument-Specific, Virus-Reactive CD4 T Cells Localize to the Cornea in Herpes Simplex Virus Interstitial Keratitis in Humans. J. Virol. 74: 10930-10938 [Abstract] [Full Text]  
  • Koelle, D. M., Schomogyi, M., McClurkan, C., Reymond, S. N., Chen, H. B. (2000). CD4 T-Cell Responses to Herpes Simplex Virus Type 2 Major Capsid Protein VP5: Comparison with Responses to Tegument and Envelope Glycoproteins. J. Virol. 74: 11422-11425 [Abstract] [Full Text]  
  • Mikloska, Z., Ruckholdt, M., Ghadiminejad, I., Dunckley, H., Denis, M., Cunningham, A. L. (2000). Monophosphoryl Lipid A and QS21 Increase CD8 T Lymphocyte Cytotoxicity to Herpes Simplex Virus-2 Infected Cell Proteins 4 and 27 Through IFN-{gamma} and IL-12 Production. J. Immunol. 164: 5167-5176 [Abstract] [Full Text]  
  • Kwok, W. W., Liu, A. W., Novak, E. J., Gebe, J. A., Ettinger, R. A., Nepom, G. T., Reymond, S. N., Koelle, D. M. (2000). HLA-DQ Tetramers Identify Epitope-Specific T Cells in Peripheral Blood of Herpes Simplex Virus Type 2-Infected Individuals: Direct Detection of Immunodominant Antigen-Responsive Cells. J. Immunol. 164: 4244-4249 [Abstract] [Full Text]  
  • Sin, J.-I., Ayyavoo, V., Boyer, J., Kim, J., Ciccarelli, R. B., Weiner, D. B. (1999). Protective immune correlates can segregate by vaccine type in a murine herpes model system. Int Immunol 11: 1763-1773 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»