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J Virol, August 1998, p. 6960-6964, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Production of Uninfectious Human Immunodeficiency Virus Type 1 Containing Viral Protein R Fused to a Single-Chain Antibody against Viral Integrase

Nobuo Okui,1,2 Noriko Kobayashi,1 and Yoshihiro Kitamura1,*

Division of Molecular Genetics, National Institute of Infectious Diseases, Gakuen, Musashimurayama,1 and Department of Urology, Faculty of Medicine, University of Tokyo, Bunkyo-ku,2 Tokyo, Japan

Received 20 January 1998/Accepted 8 May 1998

A single-chain antibody (scAb) against human immunodeficiency virus type 1 (HIV-1) integrase was expressed as a fusion protein of scAb and HIV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 293T cells. The expression did not affect virion production much but markedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appears to account for the reduced infectivity.

* Corresponding author. Mailing address: Division of Molecular Genetics, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Phone: 81-42-561-0771, ext. 371. Fax: 81-42-567-5632. E-mail: yochan{at}nih.go.jp.

J Virol, August 1998, p. 6960-6964, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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