This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by López, J. A.
Right arrow Articles by Melero, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by López, J. A.
Right arrow Articles by Melero, J. A.

 Previous Article  |  Next Article 

J Virol, August 1998, p. 6922-6928, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Antigenic Structure of Human Respiratory Syncytial Virus Fusion Glycoprotein

Juan A. López,1 Regla Bustos,1 Claes Örvell,2 Mabel Berois,3 Juan Arbiza,3 Blanca García-Barreno,1 and José A. Melero1,*

Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain1; Laboratory of Clinical Virology, Huddinge Hospital, S-141 86 Huddinge, Sweden2; and Sección de Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay3

Received 21 January 1998/Accepted 1 May 1998

New series of escape mutants of human respiratory syncytial virus were prepared with monoclonal antibodies specific for the fusion (F) protein. Sequence changes selected in the escape mutants identified two new antigenic sites (V and VI) recognized by neutralizing antibodies and a group-specific site (I) in the F1 chain of the F molecule. The new epitopes, and previously identified antigenic sites, were incorporated into a refined prediction of secondary-structure motifs to generate a detailed antigenic map of the F glycoprotein.

* Corresponding author. Mailing address: Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain. Phone: 34-91-509 7941. Fax: 34-91-5097919. E-mail: jmelero{at}isciii.es.

J Virol, August 1998, p. 6922-6928, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kovacs-Nolan, J., Mapletoft, J. W., Lawman, Z., Babiuk, L. A., van Drunen Littel-van den Hurk, S. (2009). Formulation of bovine respiratory syncytial virus fusion protein with CpG oligodeoxynucleotide, cationic host defence peptide and polyphosphazene enhances humoral and cellular responses and induces a protective type 1 immune response in mice. J. Gen. Virol. 90: 1892-1905 [Abstract] [Full Text]  
  • de Graaf, M., Osterhaus, A. D. M. E., Fouchier, R. A. M., Holmes, E. C. (2008). Evolutionary dynamics of human and avian metapneumoviruses. J. Gen. Virol. 89: 2933-2942 [Abstract] [Full Text]  
  • Ulbrandt, N. D., Ji, H., Patel, N. K., Barnes, A. S., Wilson, S., Kiener, P. A., Suzich, J., McCarthy, M. P. (2008). Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus. J. Gen. Virol. 89: 3113-3118 [Abstract] [Full Text]  
  • Johnstone, C., Guil, S., Rico, M. A., Garcia-Barreno, B., Lopez, D., Melero, J. A., Del Val, M. (2008). Relevance of viral context and diversity of antigen-processing routes for respiratory syncytial virus cytotoxic T-lymphocyte epitopes. J. Gen. Virol. 89: 2194-2203 [Abstract] [Full Text]  
  • Wu, S.-J., Schmidt, A., Beil, E. J., Day, N. D., Branigan, P. J., Liu, C., Gutshall, L. L., Palomo, C., Furze, J., Taylor, G., Melero, J. A., Tsui, P., Del Vecchio, A. M., Kruszynski, M. (2007). Characterization of the epitope for anti-human respiratory syncytial virus F protein monoclonal antibody 101F using synthetic peptides and genetic approaches. J. Gen. Virol. 88: 2719-2723 [Abstract] [Full Text]  
  • Zhao, X., Sullender, W. M. (2005). In Vivo Selection of Respiratory Syncytial Viruses Resistant to Palivizumab. J. Virol. 79: 3962-3968 [Abstract] [Full Text]  
  • Ishiguro, N., Ebihara, T., Endo, R., Ma, X., Shirotsuki, R., Ochiai, S., Ishiko, H., Kikuta, H. (2005). Immunofluorescence Assay for Detection of Human Metapneumovirus-Specific Antibodies by Use of Baculovirus-Expressed Fusion Protein. CVI 12: 202-205 [Abstract] [Full Text]  
  • Ruiz-Arguello, M. B., Martin, D., Wharton, S. A., Calder, L. J., Martin, S. R., Cano, O., Calero, M., Garcia-Barreno, B., Skehel, J. J., Melero, J. A. (2004). Thermostability of the human respiratory syncytial virus fusion protein before and after activation: implications for the membrane-fusion mechanism. J. Gen. Virol. 85: 3677-3687 [Abstract] [Full Text]  
  • Johnstone, C., de Leon, P., Medina, F., Melero, J. A., Garcia-Barreno, B., Val, M. D. (2004). Shifting immunodominance pattern of two cytotoxic T-lymphocyte epitopes in the F glycoprotein of the Long strain of respiratory syncytial virus. J. Gen. Virol. 85: 3229-3238 [Abstract] [Full Text]  
  • Easton, A. J., Domachowske, J. B., Rosenberg, H. F. (2004). Animal Pneumoviruses: Molecular Genetics and Pathogenesis. Clin. Microbiol. Rev. 17: 390-412 [Abstract] [Full Text]  
  • Smith, B. J., Lawrence, M. C., Colman, P. M. (2002). Modelling the structure of the fusion protein from human respiratory syncytial virus. Protein Eng Des Sel 15: 365-371 [Abstract] [Full Text]  
  • Zimmer, G., Trotz, I., Herrler, G. (2001). N-Glycans of F Protein Differentially Affect Fusion Activity of Human Respiratory Syncytial Virus. J. Virol. 75: 4744-4751 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»