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J Virol, August 1998, p. 6880-6883, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A New Vaccinia Virus Intermediate Transcription Factor

Patrick Sanz and Bernard Moss*

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0445

Received 6 March 1998/Accepted 18 April 1998

Transcription of the vaccinia virus genome is mediated by a virus-encoded multisubunit DNA-dependent RNA polymerase in conjunction with early-, intermediate-, and late-stage-specific factors. Previous studies indicated that two virus-encoded proteins (capping enzyme and VITF-1) and one unidentified cellular protein (VITF-2) are required for specific transcription of an intermediate promoter template in vitro. We have now extensively purified an additional virus-induced intermediate transcription factor with a native mass of approximately 100 kDa.

* Corresponding author. Mailing address: National Institutes of Health, 4 Center Dr., MSC 0445, Bethesda, MD 20892-0445. Phone: (301) 496-9869. Fax: (301) 480-1147. E-mail: bmoss{at}nih.gov.

J Virol, August 1998, p. 6880-6883, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Broyles, S. S. (2003). Vaccinia virus transcription. J. Gen. Virol. 84: 2293-2303 [Abstract] [Full Text]  
  • Sanz, P., Moss, B. (1999). Identification of a transcription factor, encoded by two vaccinia virus early genes, that regulates the intermediate stage of viral gene expression. Proc. Natl. Acad. Sci. USA 96: 2692-2697 [Abstract] [Full Text]  
  • Shors, T., Keck, J. G., Moss, B. (1999). Down Regulation of Gene Expression by the Vaccinia Virus D10 Protein. J. Virol. 73: 791-796 [Abstract] [Full Text]  


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