This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, R.
Right arrow Articles by Misra, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, R.
Right arrow Articles by Misra, V.

 Previous Article  |  Next Article 

J Virol, August 1998, p. 6291-6297, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Herpesvirus Transactivator VP16 Mimics a Human Basic Domain Leucine Zipper Protein, Luman, in Its Interaction with HCF

Rui Lu,1,2 Ping Yang,1 Sharmila Padmakumar,1 and Vikram Misra1,*

Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4,1 and Saskatchewan Health Services Utilization and Research Commission, Saskatoon, Saskatchewan S7N OW8,2 Canada

Received 13 March 1998/Accepted 28 April 1998

In human cells infected with herpes simplex virus (HSV), viral gene expression is initiated by the virion protein VP16. VP16 does not bind DNA directly but forms a multiprotein complex on the viral immediate-early gene promoters with two cellular proteins: the POU domain protein Oct-1 and host cell factor (HCF; also called C1, VCAF, and CFF). Despite its apparent role in stabilizing the VP16-induced transcription complex, the natural biological role of HCF is unclear. Only recently HCF has been implicated in control of the cell cycle. To determine the role of HCF in cells and answer why HSV has evolved an HCF-dependent mechanism for the initiation of the lytic cycle, we identified the first human ligand for HCF (R. Lu et al., Mol. Cell. Biol. 17:5117-5126, 1997). This protein, Luman, is a member of the CREB/ATF family of transcription factors that can activate transcription from promoters containing cyclic AMP response elements (CRE). Here we provide evidence that Luman and VP16 share two important structural features: an acidic activation domain and a common mechanism for binding HCF. We found that Luman, its homolog in Drosophila, dCREB-A (also known as BBF-2), and VP16 bind to HCF by a motif, (D/E)HXY(S/A), present in all three proteins. In addition, a mutation (P134S) in HCF that prevents VP16 binding also abolishes its binding to Luman and dCREB-A. We also show that while interaction with HCF is not required for the ability of Luman to activate transcription when tethered to the GAL4 promoter, it appears to be essential for Luman to activate transcription through CRE sites. These data suggest that the HCF-Luman interaction may represent a conserved mechanism for transcriptional regulation in metazoans, and HSV mimics this interaction with HCF to monitor the physiological state of the host cell.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Dr., Saskatoon, Saskatchewan S7N 5B4, Canada. Phone: (306) 966-7218. Fax: (306) 966-7244. E-mail: misra{at}duke.usask.ca.

J Virol, August 1998, p. 6291-6297, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Desmazieres, A., Charnay, P., Gilardi-Hebenstreit, P. (2009). Krox20 Controls the Transcription of Its Various Targets in the Developing Hindbrain According to Multiple Modes. J. Biol. Chem. 284: 10831-10840 [Abstract] [Full Text]  
  • Misaghi, S., Ottosen, S., Izrael-Tomasevic, A., Arnott, D., Lamkanfi, M., Lee, J., Liu, J., O'Rourke, K., Dixit, V. M., Wilson, A. C. (2009). Association of C-Terminal Ubiquitin Hydrolase BRCA1-Associated Protein 1 with Cell Cycle Regulator Host Cell Factor 1. Mol. Cell. Biol. 29: 2181-2192 [Abstract] [Full Text]  
  • Kolb, G., Kristie, T. M. (2008). Association of the Cellular Coactivator HCF-1 with the Golgi Apparatus in Sensory Neurons. J. Virol. 82: 9555-9563 [Abstract] [Full Text]  
  • Audas, T. E., Li, Y., Liang, G., Lu, R. (2008). A Novel Protein, Luman/CREB3 Reruitment Factor, Inhibits Luman Activation of the Unfolded Protein Response. Mol. Cell. Biol. 28: 3952-3966 [Abstract] [Full Text]  
  • Narayanan, A., Ruyechan, W. T., Kristie, T. M. (2007). The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection. Proc. Natl. Acad. Sci. USA 104: 10835-10840 [Abstract] [Full Text]  
  • Jang, S.-W., Kim, Y. S., Kim, Y. R., Sung, H. J., Ko, J. (2007). Regulation of Human LZIP Expression by NF-{kappa}B and Its Involvement in Monocyte Cell Migration Induced by Lkn-1. J. Biol. Chem. 282: 11092-11100 [Abstract] [Full Text]  
  • Liang, G., Audas, T. E., Li, Y., Cockram, G. P., Dean, J. D., Martyn, A. C., Kokame, K., Lu, R. (2006). Luman/CREB3 Induces Transcription of the Endoplasmic Reticulum (ER) Stress Response Protein Herp through an ER Stress Response Element. Mol. Cell. Biol. 26: 7999-8010 [Abstract] [Full Text]  
  • Vogel, J. L., Kristie, T. M. (2006). Site-specific proteolysis of the transcriptional coactivator HCF-1 can regulate its interaction with protein cofactors. Proc. Natl. Acad. Sci. USA 103: 6817-6822 [Abstract] [Full Text]  
  • Akhova, O., Bainbridge, M., Misra, V. (2005). The Neuronal Host Cell Factor-Binding Protein Zhangfei Inhibits Herpes Simplex Virus Replication. J. Virol. 79: 14708-14718 [Abstract] [Full Text]  
  • Misra, V., Rapin, N., Akhova, O., Bainbridge, M., Korchinski, P. (2005). Zhangfei Is a Potent and Specific Inhibitor of the Host Cell Factor-binding Transcription Factor Luman. J. Biol. Chem. 280: 15257-15266 [Abstract] [Full Text]  
  • Khurana, B., Kristie, T. M. (2004). A Protein Sequestering System Reveals Control of Cellular Programs by the Transcriptional Coactivator HCF-1. J. Biol. Chem. 279: 33673-33683 [Abstract] [Full Text]  
  • Yokoyama, A., Wang, Z., Wysocka, J., Sanyal, M., Aufiero, D. J., Kitabayashi, I., Herr, W., Cleary, M. L. (2004). Leukemia Proto-Oncoprotein MLL Forms a SET1-Like Histone Methyltransferase Complex with Menin To Regulate Hox Gene Expression. Mol. Cell. Biol. 24: 5639-5649 [Abstract] [Full Text]  
  • Nogueira, M. L., Wang, V. E. H., Tantin, D., Sharp, P. A., Kristie, T. M. (2004). Herpes simplex virus infections are arrested in Oct-1-deficient cells. Proc. Natl. Acad. Sci. USA 101: 1473-1478 [Abstract] [Full Text]  
  • Luciano, R. L., Wilson, A. C. (2003). HCF-1 Functions as a Coactivator for the Zinc Finger Protein Krox20. J. Biol. Chem. 278: 51116-51124 [Abstract] [Full Text]  
  • Angelastro, J. M., Ignatova, T. N., Kukekov, V. G., Steindler, D. A., Stengren, G. B., Mendelsohn, C., Greene, L. A. (2003). Regulated Expression of ATF5 Is Required for the Progression of Neural Progenitor Cells to Neurons. J. Neurosci. 23: 4590-4600 [Abstract] [Full Text]  
  • Wysocka, J., Myers, M. P., Laherty, C. D., Eisenman, R. N., Herr, W. (2003). Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes Dev. 17: 896-911 [Abstract] [Full Text]  
  • Piluso, D., Bilan, P., Capone, J. P. (2002). Host Cell Factor-1 Interacts with and Antagonizes Transactivation by the Cell Cycle Regulatory Factor Miz-1. J. Biol. Chem. 277: 46799-46808 [Abstract] [Full Text]  
  • Mahajan, S. S., Little, M. M., Vazquez, R., Wilson, A. C. (2002). Interaction of HCF-1 with a Cellular Nuclear Export Factor. J. Biol. Chem. 277: 44292-44299 [Abstract] [Full Text]  
  • Luciano, R. L., Wilson, A. C. (2002). An activation domain in the C-terminal subunit of HCF-1 is important for transactivation by VP16 and LZIP. Proc. Natl. Acad. Sci. USA 99: 13403-13408 [Abstract] [Full Text]  
  • Raggo, C., Rapin, N., Stirling, J., Gobeil, P., Smith-Windsor, E., O'Hare, P., Misra, V. (2002). Luman, the Cellular Counterpart of Herpes Simplex Virus VP16, Is Processed by Regulated Intramembrane Proteolysis. Mol. Cell. Biol. 22: 5639-5649 [Abstract] [Full Text]  
  • Higaki, S., Gebhardt, B. M., Lukiw, W. J., Thompson, H. W., Hill, J. M. (2002). Effect of Immunosuppression on Gene Expression in the HSV-1 Latently Infected Mouse Trigeminal Ganglion. IOVS 43: 1862-1869 [Abstract] [Full Text]  
  • Lee, S., Herr, W. (2001). Stabilization but Not the Transcriptional Activity of Herpes Simplex Virus VP16-Induced Complexes Is Evolutionarily Conserved among HCF Family Members. J. Virol. 75: 12402-12411 [Abstract] [Full Text]  
  • Wysocka, J., Reilly, P. T., Herr, W. (2001). Loss of HCF-1-Chromatin Association Precedes Temperature-Induced Growth Arrest of tsBN67 Cells. Mol. Cell. Biol. 21: 3820-3829 [Abstract] [Full Text]  
  • Luciano, R. L., Wilson, A. C. (2000). N-terminal transcriptional activation domain of LZIP comprises two LxxLL motifs and the Host Cell Factor-1 binding motif. Proc. Natl. Acad. Sci. USA 10.1073/pnas.190062797v1 [Abstract] [Full Text]  
  • Lu, R., Misra, V. (2000). Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16. Nucleic Acids Res 28: 2446-2454 [Abstract] [Full Text]  
  • Scarr, R. B., Smith, M. R., Beddall, M., Sharp, P. A. (2000). A Novel 50-Kilodalton Fragment of Host Cell Factor 1 (C1) in G0 Cells. Mol. Cell. Biol. 20: 3568-3575 [Abstract] [Full Text]  
  • Mahajan, S. S., Wilson, A. C. (2000). Mutations in Host Cell Factor 1 Separate Its Role in Cell Proliferation from Recruitment of VP16 and LZIP. Mol. Cell. Biol. 20: 919-928 [Abstract] [Full Text]  
  • Ajuh, P. M., Browne, G. J., Hawkes, N. A., Cohen, P. T. W., Roberts, S. G. E., Lamond, A. I. (2000). Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex. Nucleic Acids Res 28: 678-686 [Abstract] [Full Text]  
  • Lu, R., Misra, V. (2000). Potential Role for Luman, the Cellular Homologue of Herpes Simplex Virus VP16 (alpha Gene trans-Inducing Factor), in Herpesvirus Latency. J. Virol. 74: 934-943 [Abstract] [Full Text]  
  • Liu, Y., Gong, W., Huang, C. C., Herr, W., Cheng, X. (1999). Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16. Genes Dev. 13: 1692-1703 [Abstract] [Full Text]  
  • Johnson, K. M., Mahajan, S. S., Wilson, A. C. (1999). Herpes Simplex Virus Transactivator VP16 Discriminates between HCF-1 and a Novel Family Member, HCF-2. J. Virol. 73: 3930-3940 [Abstract] [Full Text]  
  • Zachos, G., Clements, B., Conner, J. (1999). Herpes Simplex Virus Type 1 Infection Stimulates p38/c-Jun N-terminal Mitogen-activated Protein Kinase Pathways and Activates Transcription Factor AP-1. J. Biol. Chem. 274: 5097-5103 [Abstract] [Full Text]  
  • Zhou, H.-J., Wong, C.-M., Chen, J.-H., Qiang, B.-Q., Yuan, J.-G., Jin, D.-Y. (2001). Inhibition of LZIP-mediated Transcription through Direct Interaction with a Novel Host Cell Factor-like Protein. J. Biol. Chem. 276: 28933-28938 [Abstract] [Full Text]  
  • Luciano, R. L., Wilson, A. C. (2000). N-terminal transcriptional activation domain of LZIP comprises two LxxLL motifs and the Host Cell Factor-1 binding motif. Proc. Natl. Acad. Sci. USA 97: 10757-10762 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»