This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feigelstock, D.
Right arrow Articles by Kaplan, G. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feigelstock, D.
Right arrow Articles by Kaplan, G. G.

 Previous Article  |  Next Article 

J Virol, July 1998, p. 6218-6222, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Polymorphisms of the Hepatitis A Virus Cellular Receptor 1 in African Green Monkey Kidney Cells Result in Antigenic Variants That Do Not React with Protective Monoclonal Antibody 190/4

Dino Feigelstock, Peter Thompson, Pravina Mattoo, and Gerardo G. Kaplan*

Laboratory of Hepatitis Viruses, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892

Received 20 January 1998/Accepted 30 March 1998

Monoclonal antibody (MAb) 190/4 blocks binding of hepatitis A virus (HAV) to the HAV cellular receptor 1 (havcr-1) and protects African green monkey kidney (AGMK) clone GL37 cells (GL37 cells) against HAV infection. BS-C-1 and CV-1 cells, two widely used AGMK cell lines, did not react with MAb 190/4 but expressed havcr-1, as judged by Western blot analysis. The cDNA coding for havcr-1 was amplified from BS-C-1 and CV-1 total cellular RNA by reverse transcription-PCR. Alignment of the amino acid sequences inferred from the cDNA nucleotide sequences showed that BS-C-1 and CV-1 havcr-1 differed from GL37 havcr-1 by having two substitutions in the Cys-rich region, N48H and K108Q, and 10 to 11 additional substitutions plus the insertion of 18 to 22 amino acids in the mucin-like region. Studies with chimeras of GL37 havcr-1 and BS-C-1 havcr-1 showed that the K108Q substitution was responsible for the lack of reaction of MAb 190/4 with BS-C-1 and CV-1 cells. Binding studies indicated that HAV bound to dog cell transfectants expressing the BS-C-1 havcr-1 as well as the GL37/BS-C-1 havcr-1 chimeras. These results indicate that antigenic variants of havcr-1 are expressed in AGMK cells and that binding of HAV to these havcr-1 variants tolerates changes in protective epitope 190/4.

* Corresponding author. Mailing address: Division of Viral Products, CBER-FDA, 8800 Rockville Pike, Bldg. 29A-NIH, Room 1D10, HFM-448, Bethesda, MD 20892. Phone: (301) 827-1870. Fax: (301) 480-5326. E-mail: gk{at}helix.nih.gov.

J Virol, July 1998, p. 6218-6222, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tami, C., Silberstein, E., Manangeeswaran, M., Freeman, G. J., Umetsu, S. E., DeKruyff, R. H., Umetsu, D. T., Kaplan, G. G. (2007). Immunoglobulin A (IgA) Is a Natural Ligand of Hepatitis A Virus Cellular Receptor 1 (HAVCR1), and the Association of IgA with HAVCR1 Enhances Virus-Receptor Interactions. J. Virol. 81: 3437-3446 [Abstract] [Full Text]  
  • Han, W. K., Alinani, A., Wu, C.-L., Michaelson, D., Loda, M., McGovern, F. J., Thadhani, R., Bonventre, J. V. (2005). Human Kidney Injury Molecule-1 Is a Tissue and Urinary Tumor Marker of Renal Cell Carcinoma. J. Am. Soc. Nephrol. 16: 1126-1134 [Abstract] [Full Text]  
  • Silberstein, E., Xing, L., van de Beek, W., Lu, J., Cheng, H., Kaplan, G. G. (2003). Alteration of Hepatitis A Virus (HAV) Particles by a Soluble Form of HAV Cellular Receptor 1 Containing the Immunoglobulin- and Mucin-Like Regions. J. Virol. 77: 8765-8774 [Abstract] [Full Text]  
  • Silberstein, E., Dveksler, G., Kaplan, G. G. (2001). Neutralization of Hepatitis A Virus (HAV) by an Immunoadhesin Containing the Cysteine-Rich Region of HAV Cellular Receptor-1. J. Virol. 75: 717-725 [Abstract] [Full Text]  
  • Cuthbert, J. A. (2001). Hepatitis A: Old and New. Clin. Microbiol. Rev. 14: 38-58 [Abstract] [Full Text]  
  • Feigelstock, D., Thompson, P., Mattoo, P., Zhang, Y., Kaplan, G. G. (1998). The Human Homolog of HAVcr-1 Codes for a Hepatitis A Virus Cellular Receptor. J. Virol. 72: 6621-6628 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»