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J Virol, July 1998, p. 6159-6163, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enhanced Gene Transfer with Fusogenic Liposomes Containing Vesicular Stomatitis Virus G Glycoprotein

Akihiro Abe,dagger Atsushi Miyanohara, and Theodore Friedmann*

Department of Pediatrics, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0634

Received 13 November 1997/Accepted 20 March 1998

Exposure of Lipofectin-DNA complexes to the partially purified G glycoprotein of the vesicular stomatitis virus envelope (VSV-G) results in loss of serum-mediated inhibition and in enhanced efficiency of gene transfer. Sucrose density gradient sedimentation analysis indicated that the VSV-G associates physically with the DNA-lipid complex to produce a VSV-G liposome. The ability to incorporate surrogate viral or cellular envelope components such as VSV-G into liposomes may allow more-efficient and possibly targeted gene delivery by lipofection, both in vitro and in vivo.

* Corresponding author. Mailing address: Department of Pediatrics, Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0634. Phone: (619) 534-4268. Fax: (619) 534-1422. E-mail: tfriedmann{at}ucsd.edu.

dagger Present address: First Department of Internal Medicine, Nagoya University School of Medicine, Showa-ku, Nagoya 466, Japan.

J Virol, July 1998, p. 6159-6163, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Diatta, A., Piver, E., Collin, C., Vaudin, P., Pages, J.-C. (2005). Semliki Forest virus-derived virus-like particles: characterization of their production and transduction pathways. J. Gen. Virol. 86: 3129-3136 [Abstract] [Full Text]  
  • Sharma, S., Miyanohara, A., Friedmann, T. (2000). Separable Mechanisms of Attachment and Cell Uptake during Retrovirus Infection. J. Virol. 74: 10790-10795 [Abstract] [Full Text]  
  • Abe, A., Chen, S.-T., Miyanohara, A., Friedmann, T. (1998). In Vitro Cell-Free Conversion of Noninfectious Moloney Retrovirus Particles to an Infectious Form by the Addition of the Vesicular Stomatitis Virus Surrogate Envelope G Protein. J. Virol. 72: 6356-6361 [Abstract] [Full Text]  


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