This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martins, C. R. F.
Right arrow Articles by Jackson, A. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martins, C. R. F.
Right arrow Articles by Jackson, A. O.

 Previous Article  |  Next Article 

J Virol, July 1998, p. 5669-5679, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sonchus Yellow Net Rhabdovirus Nuclear Viroplasms Contain Polymerase-Associated Proteins

Claudia R. F. Martins,dagger Jennifer A. Johnson, Diane M. Lawrence, Tae-Jin Choi,Dagger Anna-Maria Pisi,§ Sara L. Tobin,parallel Denise Lapidus, John D. O. Wagner,# Steven Ruzin, Kent McDonald,dagger dagger and Andrew O. Jackson*

Department of Plant and Microbial Biology, University of California, Berkeley, California 94720

Received 16 December 1997/Accepted 23 March 1998

We have initiated a study of the cytopathology of nucleorhabdoviruses by analyzing the subcellular localization of sonchus yellow net virus (SYNV) genomic and antigenomic RNAs and the encoded polymerase proteins. In situ hybridizations demonstrated that the minus-strand genomic RNA sequences are restricted to the nuclei of infected cells, while the complementary plus-strand antigenomic RNA sequences are present in both the nuclei and the cytoplasm. Immunofluorescence and immunogold labeling experiments also revealed that the nucleocapsid (N) protein and phosphoprotein (M2) are primarily localized to discrete regions within the nuclei and in virus particles that accumulate in perinuclear spaces. The N protein antiserum specifically labeled the nuclear viroplasms, whereas the M2 antiserum was more generally distributed throughout the nuclei. Antibody detection also indicated that the polymerase (L) protein is present in small amounts in the viroplasm. When the N and M2 proteins were expressed individually from the heterologous potato virus X (PVX) vector, both proteins preferentially accumulated in the nuclei. In addition, viroplasm-like inclusions formed in the nuclei of cells infected with the PVX vector containing the N gene. Fusions of the carboxy terminus of beta -glucuronidase to N and M2 resulted in staining of the nuclei of infected cells following expression from the PVX vector. Deletion analyses suggested that multiple regions of the N protein contain signals that are important for nuclear localization.

* Corresponding author. Mailing address: Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720-3102. Phone: (510) 642-3906. Fax: (510) 642-9017. E-mail: andyoj{at}uclink.berkeley.edu.

dagger Present address: Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, Brazil.

Dagger Present address: Department of Microbiology, Pukyong National University, Pusan, Korea.

§ Present address: Istituto di Patologia Vegetale, 40126 Bologna, Italy.

parallel Present address: Program for Genomics, Ethics & Society, Center for Biomedical Ethics, Stanford University, Palo Alto, Calif.

# Present address: Division of Biology, California Institute of Technology, Pasadena, Calif.

dagger dagger Present address: Electron Microscope Laboratory, University of California, Berkeley, Calif.

J Virol, July 1998, p. 5669-5679, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Goodin, M. M., Chakrabarty, R., Yelton, S., Martin, K., Clark, A., Brooks, R. (2007). Membrane and protein dynamics in live plant nuclei infected with Sonchus yellow net virus, a plant-adapted rhabdovirus. J. Gen. Virol. 88: 1810-1820 [Abstract] [Full Text]  
  • Deng, M., Bragg, J. N., Ruzin, S., Schichnes, D., King, D., Goodin, M. M., Jackson, A. O. (2007). Role of the Sonchus Yellow Net Virus N Protein in Formation of Nuclear Viroplasms. J. Virol. 81: 5362-5374 [Abstract] [Full Text]  
  • Senthil, G., Liu, H., Puram, V. G., Clark, A., Stromberg, A., Goodin, M. M. (2005). Specific and common changes in Nicotiana benthamiana gene expression in response to infection by enveloped viruses. J. Gen. Virol. 86: 2615-2625 [Abstract] [Full Text]  
  • de la Torre, M. E. S., Lopez, C., Grau, O., Garcia, M. L. (2002). RNA 2 of Citrus psorosis virus is of negative polarity and has a single open reading frame in its complementary strand. J. Gen. Virol. 83: 1777-1781 [Abstract] [Full Text]  
  • Goodin, M. M., Austin, J., Tobias, R., Fujita, M., Morales, C., Jackson, A. O. (2001). Interactions and Nuclear Import of the N and P Proteins of Sonchus Yellow Net Virus, a Plant Nucleorhabdovirus. J. Virol. 75: 9393-9406 [Abstract] [Full Text]  
  • Zhao, Y., Owens, R. A., Hammond, R. W. (2001). Use of a vector based on Potato virus X in a whole plant assay to demonstrate nuclear targeting of Potato spindle tuber viroid. J. Gen. Virol. 82: 1491-1497 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»