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J Virol, May 1998, p. 4485-4491, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Patterns of CCR5, CXCR4, and CCR3 Usage by Envelope Glycoproteins from Human Immunodeficiency Virus Type 1 Primary Isolates

Hernan A. Bazan,1 Ghalib Alkhatib,1,dagger Christopher C. Broder,2 and Edward A. Berger1,*

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445,1 and Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-47992

Received 21 August 1997/Accepted 19 January 1998

Coreceptor usage by Envs from diverse primary human immunodeficiency virus type 1 isolates was analyzed by a vaccinia virus-based expression and assay system. Usage of recombinant CCR5 and CXCR4 correlated closely with fusogenicity toward macrophages and T-cell lines expressing endogenous coreceptors. Surprisingly, recombinant CCR3 was utilized by most primary and T-cell-line-adapted Envs. Endogenous CXCR4 in macrophages was functional as a coreceptor.

* Corresponding author. Mailing address: Building 4, Room 236, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 402-2481. Fax: (301) 480-1147. E-mail: edward_berger{at}nih.gov

dagger Present address: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202.

J Virol, May 1998, p. 4485-4491, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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