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J Virol, May 1998, p. 4408-4412, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human and Simian T-Cell Leukemia Viruses Type 2 (HTLV-2 and STLV-2pan-p) Transform T Cells Independently of Jak/STAT Activation

James C. Mulloy,1,* Thi-Sau Migone,2,dagger Ted M. Ross,3,Dagger Nick Ton,1 Patrick L. Green,3,§ Warren J. Leonard,2 and Genoveffa Franchini1

Basic Research Laboratory, National Cancer Institute,1 and Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute,2 Bethesda, Maryland, and Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee3

Received 5 December 1997/Accepted 6 February 1998

Human T-lymphotropic virus type 1 (HTLV-1) and HTLV-2 differ in pathogenicity in vivo. HTLV-1 causes leukemia and neurologic and inflammatory diseases, whereas HTLV-2 is less clearly associated with human disease. Both retroviruses transform human T cells in vitro, and transformation by HTLV-1 was found to be associated with the constitutive activation of the Jak/STAT pathway. To assess whether HTLV-2 transformation may also result in constitutive activation of the Jak/STAT pathway, six interleukin-2-independent, HTLV-2-transformed T-cell lines were analyzed for the presence of activated Jak and STAT proteins by electrophoretic mobility shift assay. In addition, the phosphorylation status of Jak and STAT proteins was assessed directly by immunoprecipitation and immunoblotting with an antiphosphotyrosine antibody. Jak/STAT proteins were not found to be constitutively activated in any of the T-cell lines infected by the type 2 human and nonhuman primate viruses, suggesting that HTLV-2 and the cognate virus simian T-lymphotropic virus type 2 from Pan paniscus transform T cells in vitro by mechanisms at least partially different from those used by HTLV-1.

* Corresponding author. Mailing address: Basic Research Laboratory, Bldg. 37, Room 6A09, National Cancer Institute, National Institutes of Health, 37 Convent Dr. MSC 4255, Bethesda, MD 20892-4255. Phone: (301) 496-2655. Fax: (301) 496-8394. E-mail: jmulloy{at}helix.nih.gov.

dagger Present address: DNAX, Palo Alto, CA 94304.

Dagger Present address: Department of Genetics, Duke University Medical Center, Durham, NC 27710.

§ Present address: Departments of Veterinary Biosciences and Medical Microbiology & Immunology, The Ohio State University, Columbus, OH 43210.

J Virol, May 1998, p. 4408-4412, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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