This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wool-Lewis, R. J.
Right arrow Articles by Bates, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wool-Lewis, R. J.
Right arrow Articles by Bates, P.

 Previous Article  |  Next Article 

J Virol, April 1998, p. 3155-3160, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Ebola Virus Entry by Using Pseudotyped Viruses: Identification of Receptor-Deficient Cell Lines

Rouven J. Wool-Lewis and Paul Bates*

Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6076

Received 1 October 1997/Accepted 5 December 1997

Studies analyzing Ebola virus replication have been severely hampered by the extreme pathogenicity of this virus. To permit analysis of the host range and function of the Ebola virus glycoprotein (Ebo-GP), we have developed a system for pseudotyping these glycoproteins into murine leukemia virus (MLV). This pseudotyped virus, MLV(Ebola), can be readily concentrated to titers which exceed 5 × 106 infectious units/ml and is effectively neutralized by antibodies specific for Ebo-GP. Analysis of MLV(Ebola) infection revealed that the host range conferred by Ebo-GP is very broad, extending to cells of a variety of species. Notably, all lymphoid cell lines tested were completely resistant to infection; we speculate that this is due to the absence of a cellular receptor for Ebo-GP on B and T cells. The generation of high-titer MLV(Ebola) pseudotypes will be useful for the analysis of immune responses to Ebola virus infection, development of neutralizing antibodies, analysis of glycoprotein function, and isolation of the cellular receptor(s) for the Ebola virus.

* Corresponding author. Mailing address: Department of Microbiology, School of Medicine, University of Pennsylvania, 202B Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104-6076. Phone: (215) 573-3509. Fax: (215) 898-9557. E-mail: pbates{at}mail.med.upenn.edu.




This article has been cited by other articles:

  • Quinn, K., Brindley, M. A., Weller, M. L., Kaludov, N., Kondratowicz, A., Hunt, C. L., Sinn, P. L., McCray, P. B. Jr., Stein, C. S., Davidson, B. L., Flick, R., Mandell, R., Staplin, W., Maury, W., Chiorini, J. A. (2009). Rho GTPases Modulate Entry of Ebola Virus and Vesicular Stomatitis Virus Pseudotyped Vectors. J. Virol. 83: 10176-10186 [Abstract] [Full Text]  
  • Dube, D., Brecher, M. B., Delos, S. E., Rose, S. C., Park, E. W., Schornberg, K. L., Kuhn, J. H., White, J. M. (2009). The Primed Ebolavirus Glycoprotein (19-Kilodalton GP1,2): Sequence and Residues Critical for Host Cell Binding. J. Virol. 83: 2883-2891 [Abstract] [Full Text]  
  • Kawase, M., Shirato, K., Matsuyama, S., Taguchi, F. (2009). Protease-Mediated Entry via the Endosome of Human Coronavirus 229E. J. Virol. 83: 712-721 [Abstract] [Full Text]  
  • Wright, E., Temperton, N. J., Marston, D. A., McElhinney, L. M., Fooks, A. R., Weiss, R. A. (2008). Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison. J. Gen. Virol. 89: 2204-2213 [Abstract] [Full Text]  
  • Dube, D., Schornberg, K. L., Stantchev, T. S., Bonaparte, M. I., Delos, S. E., Bouton, A. H., Broder, C. C., White, J. M. (2008). Cell Adhesion Promotes Ebola Virus Envelope Glycoprotein-Mediated Binding and Infection. J. Virol. 82: 7238-7242 [Abstract] [Full Text]  
  • Halfmann, P., Kim, J. H., Ebihara, H., Noda, T., Neumann, G., Feldmann, H., Kawaoka, Y. (2008). Generation of biologically contained Ebola viruses. Proc. Natl. Acad. Sci. USA 105: 1129-1133 [Abstract] [Full Text]  
  • Kaletsky, R. L., Simmons, G., Bates, P. (2007). Proteolysis of the Ebola Virus Glycoproteins Enhances Virus Binding and Infectivity. J. Virol. 81: 13378-13384 [Abstract] [Full Text]  
  • Brindley, M. A., Hughes, L., Ruiz, A., McCray, P. B. Jr., Sanchez, A., Sanders, D. A., Maury, W. (2007). Ebola Virus Glycoprotein 1: Identification of Residues Important for Binding and Postbinding Events. J. Virol. 81: 7702-7709 [Abstract] [Full Text]  
  • Dominguez-Soto, A., Aragoneses-Fenoll, L., Martin-Gayo, E., Martinez-Prats, L., Colmenares, M., Naranjo-Gomez, M., Borras, F. E., Munoz, P., Zubiaur, M., Toribio, M. L., Delgado, R., Corbi, A. L. (2007). The DC-SIGN-related lectin LSECtin mediates antigen capture and pathogen binding by human myeloid cells. Blood 109: 5337-5345 [Abstract] [Full Text]  
  • Zampieri, C. A., Fortin, J.-F., Nolan, G. P., Nabel, G. J. (2007). The ERK Mitogen-Activated Protein Kinase Pathway Contributes to Ebola Virus Glycoprotein-Induced Cytotoxicity. J. Virol. 81: 1230-1240 [Abstract] [Full Text]  
  • Shimojima, M., Takada, A., Ebihara, H., Neumann, G., Fujioka, K., Irimura, T., Jones, S., Feldmann, H., Kawaoka, Y. (2006). Tyro3 family-mediated cell entry of ebola and marburg viruses.. J. Virol. 80: 10109-10116 [Abstract] [Full Text]  
  • Silvertown, J. D., Walia, J. S., Summerlee, A. J., Medin, J. A. (2006). Functional Expression of Mouse Relaxin and Mouse Relaxin-3 in the Lung from an Ebola Virus Glycoprotein-Pseudotyped Lentivirus via Tracheal Delivery. Endocrinology 147: 3797-3808 [Abstract] [Full Text]  
  • Marzi, A., Akhavan, A., Simmons, G., Gramberg, T., Hofmann, H., Bates, P., Lingappa, V. R., Pohlmann, S. (2006). The Signal Peptide of the Ebolavirus Glycoprotein Influences Interaction with the Cellular Lectins DC-SIGN and DC-SIGNR.. J. Virol. 80: 6305-6317 [Abstract] [Full Text]  
  • Kuhn, J. H., Radoshitzky, S. R., Guth, A. C., Warfield, K. L., Li, W., Vincent, M. J., Towner, J. S., Nichol, S. T., Bavari, S., Choe, H., Aman, M. J., Farzan, M. (2006). Conserved Receptor-binding Domains of Lake Victoria Marburgvirus and Zaire Ebolavirus Bind a Common Receptor. J. Biol. Chem. 281: 15951-15958 [Abstract] [Full Text]  
  • Caminschi, I., Corbett, A. J, Zahra, C., Lahoud, M., Lucas, K. M, Sofi, M., Vremec, D., Gramberg, T., Pohlmann, S., Curtis, J., Handman, E., van Dommelen, S. L H, Fleming, P., Degli-Esposti, M. A, Shortman, K., Wright, M. D (2006). Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol 18: 741-753 [Abstract] [Full Text]  
  • Bar, S., Takada, A., Kawaoka, Y., Alizon, M. (2006). Detection of Cell-Cell Fusion Mediated by Ebola Virus Glycoproteins. J. Virol. 80: 2815-2822 [Abstract] [Full Text]  
  • Amberg, S. M., Netter, R. C., Simmons, G., Bates, P. (2006). Expanded Tropism and Altered Activation of a Retroviral Glycoprotein Resistant to an Entry Inhibitor Peptide. J. Virol. 80: 353-359 [Abstract] [Full Text]  
  • Wahl-Jensen, V. M., Afanasieva, T. A., Seebach, J., Stroher, U., Feldmann, H., Schnittler, H.-J. (2005). Effects of Ebola Virus Glycoproteins on Endothelial Cell Activation and Barrier Function. J. Virol. 79: 10442-10450 [Abstract] [Full Text]  
  • Chandran, K., Sullivan, N. J., Felbor, U., Whelan, S. P., Cunningham, J. M. (2005). Endosomal Proteolysis of the Ebola Virus Glycoprotein Is Necessary for Infection. Science 308: 1643-1645 [Abstract] [Full Text]  
  • Kunz, S., Rojek, J. M., Perez, M., Spiropoulou, C. F., Oldstone, M. B. A. (2005). Characterization of the Interaction of Lassa Fever Virus with Its Cellular Receptor {alpha}-Dystroglycan. J. Virol. 79: 5979-5987 [Abstract] [Full Text]  
  • Manicassamy, B., Wang, J., Jiang, H., Rong, L. (2005). Comprehensive Analysis of Ebola Virus GP1 in Viral Entry. J. Virol. 79: 4793-4805 [Abstract] [Full Text]  
  • Yang, Z.-y., Werner, H. C., Kong, W.-p., Leung, K., Traggiai, E., Lanzavecchia, A., Nabel, G. J. (2005). Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses. Proc. Natl. Acad. Sci. USA 102: 797-801 [Abstract] [Full Text]  
  • Kolokoltsov, A. A., Weaver, S. C., Davey, R. A. (2005). Efficient Functional Pseudotyping of Oncoretroviral and Lentiviral Vectors by Venezuelan Equine Encephalitis Virus Envelope Proteins. J. Virol. 79: 756-763 [Abstract] [Full Text]  
  • Yonezawa, A., Cavrois, M., Greene, W. C. (2005). Studies of Ebola Virus Glycoprotein-Mediated Entry and Fusion by Using Pseudotyped Human Immunodeficiency Virus Type 1 Virions: Involvement of Cytoskeletal Proteins and Enhancement by Tumor Necrosis Factor Alpha. J. Virol. 79: 918-926 [Abstract] [Full Text]  
  • Netter, R. C., Amberg, S. M., Balliet, J. W., Biscone, M. J., Vermeulen, A., Earp, L. J., White, J. M., Bates, P. (2004). Heptad Repeat 2-Based Peptides Inhibit Avian Sarcoma and Leukosis Virus Subgroup A Infection and Identify a Fusion Intermediate. J. Virol. 78: 13430-13439 [Abstract] [Full Text]  
  • Yi, L., Li, Z., Yuan, K., Qu, X., Chen, J., Wang, G., Zhang, H., Luo, H., Zhu, L., Jiang, P., Chen, L., Shen, Y., Luo, M., Zuo, G., Hu, J., Duan, D., Nie, Y., Shi, X., Wang, W., Han, Y., Li, T., Liu, Y., Ding, M., Deng, H., Xu, X. (2004). Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells. J. Virol. 78: 11334-11339 [Abstract] [Full Text]  
  • Barnard, R. J. O., Narayan, S., Dornadula, G., Miller, M. D., Young, J. A. T. (2004). Low pH Is Required for Avian Sarcoma and Leukosis Virus Env-Dependent Viral Penetration into the Cytosol and Not for Viral Uncoating. J. Virol. 78: 10433-10441 [Abstract] [Full Text]  
  • Giroglou, T., Cinatl, J. Jr., Rabenau, H., Drosten, C., Schwalbe, H., Doerr, H. W., von Laer, D. (2004). Retroviral Vectors Pseudotyped with Severe Acute Respiratory Syndrome Coronavirus S Protein. J. Virol. 78: 9007-9015 [Abstract] [Full Text]  
  • Yang, Z.-Y., Huang, Y., Ganesh, L., Leung, K., Kong, W.-P., Schwartz, O., Subbarao, K., Nabel, G. J. (2004). pH-Dependent Entry of Severe Acute Respiratory Syndrome Coronavirus Is Mediated by the Spike Glycoprotein and Enhanced by Dendritic Cell Transfer through DC-SIGN. J. Virol. 78: 5642-5650 [Abstract] [Full Text]  
  • Melikyan, G. B., Barnard, R. J. O., Markosyan, R. M., Young, J. A. T., Cohen, F. S. (2004). Low pH Is Required for Avian Sarcoma and Leukosis Virus Env-Induced Hemifusion and Fusion Pore Formation but Not for Pore Growth. J. Virol. 78: 3753-3762 [Abstract] [Full Text]  
  • Simmons, G., Reeves, J. D., Rennekamp, A. J., Amberg, S. M., Piefer, A. J., Bates, P. (2004). Characterization of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike glycoprotein-mediated viral entry. Proc. Natl. Acad. Sci. USA 101: 4240-4245 [Abstract] [Full Text]  
  • Takada, A., Fujioka, K., Tsuiji, M., Morikawa, A., Higashi, N., Ebihara, H., Kobasa, D., Feldmann, H., Irimura, T., Kawaoka, Y. (2004). Human Macrophage C-Type Lectin Specific for Galactose and N-Acetylgalactosamine Promotes Filovirus Entry. J. Virol. 78: 2943-2947 [Abstract] [Full Text]  
  • Zuckert, W. R., Lloyd, J. E., Stewart, P. E., Rosa, P. A., Barbour, A. G. (2004). Cross-Species Surface Display of Functional Spirochetal Lipoproteins by Recombinant Borrelia burgdorferi. Infect. Immun. 72: 1463-1469 [Abstract] [Full Text]  
  • Simmons, G., Rennekamp, A. J., Chai, N., Vandenberghe, L. H., Riley, J. L., Bates, P. (2003). Folate Receptor Alpha and Caveolae Are Not Required for Ebola Virus Glycoprotein-Mediated Viral Infection. J. Virol. 77: 13433-13438 [Abstract] [Full Text]  
  • Lasala, F., Arce, E., Otero, J. R., Rojo, J., Delgado, R. (2003). Mannosyl Glycodendritic Structure Inhibits DC-SIGN-Mediated Ebola Virus Infection in cis and in trans. Antimicrob. Agents Chemother. 47: 3970-3972 [Abstract] [Full Text]  
  • Takada, A., Feldmann, H., Ksiazek, T. G., Kawaoka, Y. (2003). Antibody-Dependent Enhancement of Ebola Virus Infection. J. Virol. 77: 7539-7544 [Abstract] [Full Text]  
  • Sinn, P. L., Hickey, M. A., Staber, P. D., Dylla, D. E., Jeffers, S. A., Davidson, B. L., Sanders, D. A., McCray, P. B. Jr. (2003). Lentivirus Vectors Pseudotyped with Filoviral Envelope Glycoproteins Transduce Airway Epithelia from the Apical Surface Independently of Folate Receptor Alpha. J. Virol. 77: 5902-5910 [Abstract] [Full Text]  
  • Earp, L. J., Delos, S. E., Netter, R. C., Bates, P., White, J. M. (2003). The Avian Retrovirus Avian Sarcoma/Leukosis Virus Subtype A Reaches the Lipid Mixing Stage of Fusion at Neutral pH. J. Virol. 77: 3058-3066 [Abstract] [Full Text]  
  • Takada, A., Feldmann, H., Stroeher, U., Bray, M., Watanabe, S., Ito, H., McGregor, M., Kawaoka, Y. (2002). Identification of Protective Epitopes on Ebola Virus Glycoprotein at the Single Amino Acid Level by Using Recombinant Vesicular Stomatitis Viruses. J. Virol. 77: 1069-1074 [Abstract] [Full Text]  
  • Jeffers, S. A., Sanders, D. A., Sanchez, A. (2002). Covalent Modifications of the Ebola Virus Glycoprotein. J. Virol. 76: 12463-12472 [Abstract] [Full Text]  
  • Alvarez, C. P., Lasala, F., Carrillo, J., Muniz, O., Corbi, A. L., Delgado, R. (2002). C-Type Lectins DC-SIGN and L-SIGN Mediate Cellular Entry by Ebola Virus in cis and in trans. J. Virol. 76: 6841-6844 [Abstract] [Full Text]  
  • Empig, C. J., Goldsmith, M. A. (2002). Association of the Caveola Vesicular System with Cellular Entry by Filoviruses. J. Virol. 76: 5266-5270 [Abstract] [Full Text]  
  • Simmons, G., Wool-Lewis, R. J., Baribaud, F., Netter, R. C., Bates, P. (2002). Ebola Virus Glycoproteins Induce Global Surface Protein Down-Modulation and Loss of Cell Adherence. J. Virol. 76: 2518-2528 [Abstract] [Full Text]  
  • Sung, V. M.-H., Lai, M. M. C. (2002). Murine Retroviral Pseudotype Virus Containing Hepatitis B Virus Large and Small Surface Antigens Confers Specific Tropism for Primary Human Hepatocytes: a Potential Liver-Specific Targeting System. J. Virol. 76: 912-917 [Abstract] [Full Text]  
  • Feldmann, H., Volchkov, V. E., Volchkova, V. A., Stroher, U., Klenk, H.-D. (2001). Biosynthesis and role of filoviral glycoproteins. J. Gen. Virol. 82: 2839-2848 [Full Text]  
  • Bruett, L., Clements, J. E. (2001). Functional Murine Leukemia Virus Vectors Pseudotyped with the Visna Virus Envelope Show Expanded Visna Virus Cell Tropism. J. Virol. 75: 11464-11473 [Abstract] [Full Text]  
  • Perez, M., Watanabe, M., Whitt, M. A., de la Torre, J. C. (2001). N-Terminal Domain of Borna Disease Virus G (p56) Protein Is Sufficient for Virus Receptor Recognition and Cell Entry. J. Virol. 75: 7078-7085 [Abstract] [Full Text]  
  • Jasenosky, L. D., Neumann, G., Lukashevich, I., Kawaoka, Y. (2001). Ebola Virus VP40-Induced Particle Formation and Association with the Lipid Bilayer. J. Virol. 75: 5205-5214 [Abstract] [Full Text]  
  • Christodoulopoulos, I., Cannon, P. M. (2001). Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus Vectors. J. Virol. 75: 4129-4138 [Abstract] [Full Text]  
  • Chazal, N., Singer, G., Aiken, C., Hammarskjöld, M.-L., Rekosh, D. (2001). Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity. J. Virol. 75: 4014-4018 [Abstract] [Full Text]  
  • Sharkey, C. M., North, C. L., Kuhn, R. J., Sanders, D. A. (2001). Ross River Virus Glycoprotein-Pseudotyped Retroviruses and Stable Cell Lines for Their Production. J. Virol. 75: 2653-2659 [Abstract] [Full Text]  
  • Takada, A., Watanabe, S., Okazaki, K., Kida, H., Kawaoka, Y. (2001). Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein. J. Virol. 75: 2324-2330 [Abstract] [Full Text]  
  • Ito, H., Watanabe, S., Takada, A., Kawaoka, Y. (2001). Ebola Virus Glycoprotein: Proteolytic Processing, Acylation, Cell Tropism, and Detection of Neutralizing Antibodies. J. Virol. 75: 1576-1580 [Abstract] [Full Text]  
  • Zeilfelder, U., Bosch, V. (2001). Properties of Wild-Type, C-Terminally Truncated, and Chimeric Maedi-Visna Virus Glycoprotein and Putative Pseudotyping of Retroviral Vector Particles. J. Virol. 75: 548-555 [Abstract] [Full Text]  
  • Harty, R. N., Brown, M. E., Wang, G., Huibregtse, J., Hayes, F. P. (2000). A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding. Proc. Natl. Acad. Sci. USA 10.1073/pnas.250277297v1 [Abstract] [Full Text]  
  • Watanabe, S., Takada, A., Watanabe, T., Ito, H., Kida, H., Kawaoka, Y. (2000). Functional Importance of the Coiled-Coil of the Ebola Virus Glycoprotein. J. Virol. 74: 10194-10201 [Abstract] [Full Text]  
  • Damico, R., Bates, P. (2000). Soluble Receptor-Induced Retroviral Infection of Receptor-Deficient Cells. J. Virol. 74: 6469-6475 [Abstract] [Full Text]  
  • Chan, S. Y., Speck, R. F., Ma, M. C., Goldsmith, M. A. (2000). Distinct Mechanisms of Entry by Envelope Glycoproteins of Marburg and Ebola (Zaire) Viruses. J. Virol. 74: 4933-4937 [Abstract] [Full Text]  
  • Balliet, J. W., Gendron, K., Bates, P. (2000). Mutational Analysis of the Subgroup A Avian Sarcoma and Leukosis Virus Putative Fusion Peptide Domain. J. Virol. 74: 3731-3739 [Abstract] [Full Text]  
  • Blond, J.-L., Lavillette, D., Cheynet, V., Bouton, O., Oriol, G., Chapel-Fernandes, S., Mandrand, B., Mallet, F., Cosset, F.-L. (2000). An Envelope Glycoprotein of the Human Endogenous Retrovirus HERV-W Is Expressed in the Human Placenta and Fuses Cells Expressing the Type D Mammalian Retrovirus Receptor. J. Virol. 74: 3321-3329 [Abstract] [Full Text]  
  • Delos, S. E., Gilbert, J. M., White, J. M. (2000). The Central Proline of an Internal Viral Fusion Peptide Serves Two Important Roles. J. Virol. 74: 1686-1693 [Abstract] [Full Text]  
  • Ito, H., Watanabe, S., Sanchez, A., Whitt, M. A., Kawaoka, Y. (1999). Mutational Analysis of the Putative Fusion Domain of Ebola Virus Glycoprotein. J. Virol. 73: 8907-8912 [Abstract] [Full Text]  
  • Boerger, A. L., Snitkovsky, S., Young, J. A. T. (1999). Retroviral vectors preloaded with a viral receptor-ligand bridge protein are targeted to specific cell types. Proc. Natl. Acad. Sci. USA 96: 9867-9872 [Abstract] [Full Text]  
  • Wool-Lewis, R. J., Bates, P. (1999). Endoproteolytic Processing of the Ebola Virus Envelope Glycoprotein: Cleavage Is Not Required for Function. J. Virol. 73: 1419-1426 [Abstract] [Full Text]  
  • Harty, R. N., Brown, M. E., Wang, G., Huibregtse, J., Hayes, F. P. (2000). A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding. Proc. Natl. Acad. Sci. USA 97: 13871-13876 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»