This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mikhailov, V. S.
Right arrow Articles by Maeda, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mikhailov, V. S.
Right arrow Articles by Maeda, S.

 Previous Article  |  Next Article 

J Virol, April 1998, p. 3107-3116, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bombyx mori Nucleopolyhedrovirus Encodes a DNA-Binding Protein Capable of Destabilizing Duplex DNA

Victor S. Mikhailov,1,2,* Alla L. Mikhailova,2 Masashi Iwanaga,2 Sumiko Gomi,2 and Susumu Maeda2,3

N. K. Koltzov Institute of Developmental Biology, Moscow, Russia1; Laboratory of Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN), Wako, Japan2; and Department of Entomology, University of California, Davis, California 956163

Received 21 October 1997/Accepted 12 December 1997

A DNA-binding protein (designated DBP) with an apparent molecular mass of 38 kDa was purified to homogeneity from BmN cells (derived from Bombyx mori) infected with the B. mori nucleopolyhedrovirus (BmNPV). Six peptides obtained after digestion of the isolated protein with Achromobacter protease I were partially or completely sequenced. The determined amino acid sequences indicated that DBP was encoded by an open reading frame (ORF16) located at nucleotides (nt) 16189 to 17139 in the BmNPV genome (GenBank accession no. L33180). This ORF (designated dbp) is a homolog of Autographa californica multicapsid NPV ORF25, whose product has not been identified. BmNPV DBP is predicted to contain 317 amino acids (calculated molecular mass of 36.7 kDa) and to have an isoelectric point of 7.8. DBP showed a tendency to multimerization in the course of purification and was found to bind preferentially to single-stranded DNA. When bound to oligonucleotides, DBP protected them from hydrolysis by phage T4 DNA polymerase-associated 3'right-arrow5' exonuclease. The sizes of the protected fragments indicated that a binding site size for DBP is about 30 nt per protein monomer. DBP, but not BmNPV LEF-3, was capable of unwinding partial DNA duplexes in an in vitro system. This helix-destabilizing ability is consistent with the prediction that DBP functions as a single-stranded DNA binding protein in virus replication.

* Corresponding author. Mailing address: Laboratory of Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN), Waco, Japan. Phone: 81 (48) 467-9584. Fax: 81 (48) 462-4678: E-mail: vsmikha{at}postman.riken.go.jp.




This article has been cited by other articles:

  • Chen, Y.-R., Wu, C.-Y., Lee, S.-T., Wu, Y.-J., Lo, C.-F., Tsai, M.-F., Wang, C.-H. (2008). Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus. J. Gen. Virol. 89: 2315-2330 [Abstract] [Full Text]  
  • Quadt, I., van Lent, J. W. M., Knebel-Morsdorf, D. (2007). Studies of the Silencing of Baculovirus DNA Binding Protein. J. Virol. 81: 6122-6127 [Abstract] [Full Text]  
  • Oliveira, J. V. d. C., Wolff, J. L. C., Garcia-Maruniak, A., Ribeiro, B. M., de Castro, M. E. B., de Souza, M. L., Moscardi, F., Maruniak, J. E., Zanotto, P. M. d. A. (2006). Genome of the most widely used viral biopesticide: Anticarsia gemmatalis multiple nucleopolyhedrovirus.. J. Gen. Virol. 87: 3233-3250 [Abstract] [Full Text]  
  • Chen, Z., Carstens, E. B. (2005). Identification of Domains in Autographa californica Multiple Nucleopolyhedrovirus Late Expression Factor 3 Required for Nuclear Transport of P143. J. Virol. 79: 10915-10922 [Abstract] [Full Text]  
  • Mikhailov, V. S., Okano, K., Rohrmann, G. F. (2005). The Redox State of the Baculovirus Single-stranded DNA-binding Protein LEF-3 Regulates Its DNA Binding, Unwinding, and Annealing Activities. J. Biol. Chem. 280: 29444-29453 [Abstract] [Full Text]  
  • de Jong, J. G., Lauzon, H. A. M., Dominy, C., Poloumienko, A., Carstens, E. B., Arif, B. M., Krell, P. J. (2005). Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome. J. Gen. Virol. 86: 929-943 [Abstract] [Full Text]  
  • Garcia-Maruniak, A., Maruniak, J. E., Zanotto, P. M. A., Doumbouya, A. E., Liu, J.-C., Merritt, T. M., Lanoie, J. S. (2004). Sequence Analysis of the Genome of the Neodiprion sertifer Nucleopolyhedrovirus. J. Virol. 78: 7036-7051 [Abstract] [Full Text]  
  • Mainz, D., Quadt, I., Knebel-Morsdorf, D. (2002). Nuclear IE2 Structures Are Related to Viral DNA Replication Sites during Baculovirus Infection. J. Virol. 76: 5198-5207 [Abstract] [Full Text]  
  • Chen, X., IJkel, W. F. J., Tarchini, R., Sun, X., Sandbrink, H., Wang, H., Peters, S., Zuidema, D., Lankhorst, R. K., Vlak, J. M., Hu, Z. (2001). The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J. Gen. Virol. 82: 241-257 [Abstract] [Full Text]  
  • Zemskov, E. A., Kang, W., Maeda, S. (2000). Evidence for Nucleic Acid Binding Ability and Nucleosome Association of Bombyx mori Nucleopolyhedrovirus BRO Proteins. J. Virol. 74: 6784-6789 [Abstract] [Full Text]  
  • IJkel, W. F. J., van Strien, E. A., Heldens, J. G. M., Broer, R., Zuidema, D., Goldbach, R. W., Vlak, J. M. (1999). Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J. Gen. Virol. 80: 3289-3304 [Abstract] [Full Text]  
  • Hang, X., Guarino, L. A. (1999). Purification of Autographa californica nucleopolyhedrovirus DNA polymerase from infected insect cells. J. Gen. Virol. 80: 2519-2526 [Abstract] [Full Text]  
  • Gomi, S, Majima, K, Maeda, S (1999). Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J. Gen. Virol. 80: 1323-1337 [Abstract]  
  • Okano, K., Mikhailov, V. S., Maeda, S. (1999). Colocalization of Baculovirus IE-1 and Two DNA-Binding Proteins, DBP and LEF-3, to Viral Replication Factories. J. Virol. 73: 110-119 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»