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J Virol, April 1998, p. 3072-3075, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Hepatitis G Virus Replication Sites by Using Highly Strand-Specific Tth-Based Reverse Transcriptase PCR

Tomasz Laskus,1,* Marek Radkowski,1 Lian-Fu Wang,1 Hugo Vargas,2 and Jorge Rakela1,2

Division of Transplantation Medicine, Thomas E. Starzl Transplantation Institute, University of Pittsburgh,1 and Division of Gastroenterology and Hepatology, University of Pittsburgh Medical Center,2 Pittsburgh, Pennsylvania

Received 3 November 1997/Accepted 17 December 1997

The replication sites of the recently discovered hepatitis G virus (HGV) remain unknown. Using highly strand-specific Tth-based reverse transcriptase PCR, we searched for the presence of viral RNA negative strand in multiple autopsy tissues from four patients with AIDS and in peripheral blood mononuclear cells from six other human immunodeficiency virus-positive patients. Negative-strand HGV RNA was detected in three of four bone marrow samples, in two of two spleen samples, and in one of four liver tissue samples. However, the specific cellular site of replication within the positive tissues was not determined. This study does not support HGV as a primary hepatotropic virus.

* Corresponding author. Mailing address: Division of Transplant Medicine, University of Pittsburgh Medical Center, 301 Lhormer Bldg., 200 Lothrop St., Pittsburgh, PA 15213. Phone: (412) 624-0287. Fax: (412) 647-9672.




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