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J Virol, February 1998, p. 1699-1703, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Construction and Characterization of a Hybrid Mouse Mammary Tumor Virus/Murine Leukemia Virus-Based Retroviral Vector

Robert M. Saller,1,2 Feride Öztürk,1,3 Brian Salmons,2 and Walter H. Günzburg1,3,*

Institut für Molekulare Virologie, GSF-Forschungszentrum für Umwelt und Gesundheit, D-85758 Oberschleissheim,1 and Bavarian Nordic Research Institute, D-80807 Munich,2 Germany, and Institute of Virology, University of Veterinary Sciences, A-1210 Vienna, Austria3

Received 6 June 1997/Accepted 21 October 1997

Mouse mammary tumor virus (MMTV)-based vectors are characterized by low titers. In an effort to transfer MMTV-specific regulation of gene expression to a more efficient murine leukemia virus (MLV) vector, we have replaced the complete 3' U3 region of MLV with the complete U3 region of MMTV. Virus titers were not significantly affected by this modification, there was no impairment of reverse transcription and integration, and after infection of cells, the MMTV promoter is duplicated and translocated to the 5' long terminal repeat, resulting in glucocorticoid-regulatable RNA expression.

* Corresponding author. Mailing address: Institute of Virology, University of Veterinary Sciences, Josef-Baumann-Gasse 1, A-1210 Vienna, Austria. Phone: 43-1-25077-2301. Fax: 43-1-25077-2390. E-mail: walter.guenzburg{at}vu-wien.ac.at.




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  • Schon, U., Diem, O., Leitner, L., Gunzburg, W. H., Mager, D. L., Salmons, B., Leib-Mosch, C. (2009). Human Endogenous Retroviral Long Terminal Repeat Sequences as Cell Type-Specific Promoters in Retroviral Vectors. J. Virol. 83: 12643-12650 [Abstract] [Full Text]  
  • Hlavaty, J., Stracke, A., Klein, D., Salmons, B., Gunzburg, W. H., Renner, M. (2004). Multiple Modifications Allow High-Titer Production of Retroviral Vectors Carrying Heterologous Regulatory Elements. J. Virol. 78: 1384-1392 [Abstract] [Full Text]  


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