This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Séguin, B.
Right arrow Articles by Cochrane, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Séguin, B.
Right arrow Articles by Cochrane, A.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 9503-9513, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Control of Human Immunodeficiency Virus Type 1 RNA Metabolism: Role of Splice Sites and Intron Sequences in Unspliced Viral RNA Subcellular Distribution

Béatrice Séguin,1 Alfredo Staffa,2 and Alan Cochrane1,*

Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario,1 and Department of Microbiology and Immunology, McGill University, Montreal, Quebec,2 Canada

Received 19 June 1998/Accepted 4 August 1998

In the course of examining the various factors which affect the metabolism of human immunodeficiency virus type 1 (HIV-1) RNA, we examined the role of intron sequences and splice sites in determining the subcellular distribution of the RNA. Using in situ hybridization, we demonstrated that in the absence of Rev, unspliced RNA generated with an HIV-1 env expression construct displayed discrete localization in the nucleus, coincident with the location of the gene and not associated with SC35-containing nuclear speckles. Expression of Rev resulted in a disperse signal for the unspliced RNA throughout both the nucleus and the cytoplasm. Subsequent fractionation of the nucleus revealed that the majority of unspliced viral RNA within the nucleus is associated with the nuclear matrix and that upon expression of Rev, a small proportion of the unspliced RNA is found within the nucleoplasm. Mutations which altered splice site utilization did not alter the sequestration of unspliced RNA into discrete nuclear regions. In contrast, a 2.2-kb deletion of intron sequence resulted in a shift from discrete regions within the nucleus to a disperse signal throughout the cell, indicating that intron sequences, and not just splice sites, are required for the observed nuclear sequestration of unspliced viral RNA.

* Corresponding author. Mailing address: Dept. of Molecular and Medical Genetics, Medical Sciences Bldg., University of Toronto, Toronto, Ontario M5S 1A8, Canada. Phone: (416) 978-2500. Fax: (416) 978-6885. E-mail: alan.cochrane{at}utoronto.ca.

Journal of Virology, December 1998, p. 9503-9513, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • MCLAREN, M., ASAI, K., COCHRANE, A. (2004). A novel function for Sam68: Enhancement of HIV-1 RNA 3' end processing. RNA 10: 1119-1129 [Abstract] [Full Text]  
  • Mabit, H., Knaust, A., Breiner, K. M., Schaller, H. (2003). Nuclear Localization of the Duck Hepatitis B Virus Capsid Protein: Detection and Functional Implications of Distinct Subnuclear Bodies in a Compartment Associated with RNA Synthesis and Maturation. J. Virol. 77: 2157-2164 [Abstract] [Full Text]  
  • Cmarko, D., Boe, S.-O., Scassellati, C., Szilvay, A. M., Davanger, S., Fu, X.-D., Haukenes, G., Kalland, K.-H., Fakan, S. (2002). Rev Inhibition Strongly Affects Intracellular Distribution of Human Immunodeficiency Virus Type 1 RNAs. J. Virol. 76: 10473-10484 [Abstract] [Full Text]  
  • Pongoski, J., Asai, K., Cochrane, A. (2002). Positive and Negative Modulation of Human Immunodeficiency Virus Type 1 Rev Function by cis and trans Regulators of Viral RNA Splicing. J. Virol. 76: 5108-5120 [Abstract] [Full Text]  
  • Soros, V. B., Carvajal, H. V., Richard, S., Cochrane, A. W. (2001). Inhibition of Human Immunodeficiency Virus Type 1 Rev Function by a Dominant-Negative Mutant of Sam68 through Sequestration of Unspliced RNA at Perinuclear Bundles. J. Virol. 75: 8203-8215 [Abstract] [Full Text]  
  • Graf, M., Bojak, A., Deml, L., Bieler, K., Wolf, H., Wagner, R. (2000). Concerted Action of Multiple cis-Acting Sequences Is Required for Rev Dependence of Late Human Immunodeficiency Virus Type 1 Gene Expression. J. Virol. 74: 10822-10826 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»