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Journal of Virology, November 1998, p. 9370-9373, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The P2 Protein of Rice Dwarf Phytoreovirus Is Required for Adsorption of the Virus to Cells of the Insect Vector

Toshihiro Omura,* Jin Yan, Boxiong Zhong,dagger Masato Wada,Dagger Yafeng Zhu,§ Masatoshi Tomaru,parallel Wakako Maruyama,# Akira Kikuchi, Yasuo Watanabe, Ikuo Kimura, and Hiroyuki Hibinodagger dagger

National Agriculture Research Center, Tsukuba, Ibaraki 305, Japan

Received 14 May 1998/Accepted 27 July 1998

Intact particles of rice dwarf phytoreovirus adsorbed to and entered monolayer-cultured cells of the insect vector Nephotettix cincticeps and multiplied within the cells. Particles that lacked the P2 protein neither attached to nor infected such cells. Furthermore, P2-free particles obtained from a transmission-competent isolate of the virus were unable to infect insect vectors that had been allowed to feed on these virus particles through a membrane. However, when such virus particles were injected into insects via a glass capillary tube they successfully infected the insects, which became able to transmit the virus. These results support the hypothesis that, while P2-free particles can neither interact with nor infect cells in the intestinal tract of the insect vector, they do retain the ability to infect such cells when physically introduced into the hemolymph by injection.

* Corresponding author. Mailing address, National Agriculture Research Center, Tsukuba, Ibaraki 305, Japan. Phone: 81-298-38-3932. Fax: 81-298-38-8929. E-mail: toomura{at}narc.affrc.go.jp.

dagger Present address: Zhejiang Agricultural University, Hangzhou, China.

Dagger Present address: Apple Research Center, National Institute of Fruit Tree Research, Morioka 020-01, Japan.

§ Present address: DNAVEC Research Inc., Kannondai, Tsukuba, Ibaraki 305, Japan.

parallel Present address: Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan.

# Present address: Hokkaido National Agricultural Experiment Station, Memuro, Hokkaido 082-0071, Japan.

dagger dagger Present address: National Institute of Agro-Environmental Sciences, Tsukuba, Ibaraki 305, Japan.

Journal of Virology, November 1998, p. 9370-9373, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wei, T., Hibino, H., Omura, T. (2008). Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells. J. Gen. Virol. 89: 2915-2920 [Abstract] [Full Text]  
  • Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y. (2007). The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc. Natl. Acad. Sci. USA 104: 19547-19552 [Abstract] [Full Text]  
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