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Journal of Virology, November 1998, p. 8725-8730, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Definition and Distribution Analysis of Glycoprotein B Gene Alleles of Human Herpesvirus 7

Michael Franti, Jean-Thierry Aubin, Laurent Poirel, Agnes Gautheret-Dejean, Daniel Candotti, Jean-Marie Huraux, and Henri Agut*

Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpêtrière, 75651 Paris Cedex 13, France

Received 28 May 1998/Accepted 7 July 1998

As for other herpesviruses, glycoprotein B (gB) of human herpesvirus 7 (HHV-7) is believed to play a major role in virus infection and as a target of the host immunogenic response. Using nested PCR, we amplified the whole HHV-7 gB gene from 108 human peripheral blood mononuclear cell samples and studied its variability. By means of restriction fragment length polymorphism (RFLP) analysis, three distinct patterns, designated I, II, and III, were defined and detected at frequencies of 93, 5, and 2%, respectively. Determination of the nucleotide sequence allowed us to recognize five critical positions in the gB gene with six specific combinations of point changes at these positions. These combinations were gB alleles A, B, C, D, E, and F. Alleles D and E corresponded to RFLP patterns II and III, respectively, while the other four alleles corresponded to RFLP pattern I. Identical gB alleles were detected in serial samples as well as in paired samples of blood and saliva from the same individuals, except for one case. In contrast, the distribution of gB alleles differed according to the geographical origin of the human samples: C was the most frequent allele in both African and Caribbean samples, whereas F was the most frequent allele in European ones. Although none of the allele-specific nucleotide changes induced any modification at the protein level, the definition of gB alleles provided convenient viral markers for the study of both HHV-7 infections and human population genetics.

* Corresponding author. Mailing address: Laboratoire de Virologie, C.E.R.V.I., Hôpital Pitié-Salpêtrière, 83 bd de l'Hôpital, 75651 Paris Cedex 13, France. Phone: 33.1.42.17.74.01. Fax: 33.1.42.17.74.11. E-mail: henri.agut{at}psl.ap-hop-paris.fr.

Journal of Virology, November 1998, p. 8725-8730, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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