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Journal of Virology, October 1998, p. 8384-8391, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Proviral Structure, Chromosomal Location, and Expression of HERV-K-T47D, a Novel Human Endogenous Retrovirus Derived from T47D Particles

Wolfgang Seifarth,1,* Corinna Baust,1 Andreas Murr,1,2 Heyko Skladny,1 Frank Krieg-Schneider,1 Jürgen Blusch,3 Thomas Werner,3 Rüdiger Hehlmann,1 and Christine Leib-Mösch1,2

Medical Clinic III, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68305 Mannheim,1 and Institutes of Molecular Virology2 and Mammalian Genetics,3 GSF-National Research Center for Environment and Health, D-85764 Neuherberg, Germany

Received 17 February 1998/Accepted 12 June 1998

We previously described that type B retrovirus-like particles released from the human mammary carcinoma cell line T47D are pseudotypes and package retroviral RNA of different origins (W. Seifarth, H. Skladny, F. Krieg-Schneider, A. Reichert, R. Hehlmann, and C. Leib-Mösch, J. Virol. 69:6408-6416, 1995). One preferentially packaged retroviral sequence, ERV-MLN, has now been used to isolate the corresponding full-length provirus from a human genomic library. The 9,315-bp proviral genome comprises a complete retroviral structure except for a 3' long terminal repeat (LTR) truncation. A lysine tRNA primer-binding site and phylogenetic analyses assign this human endogenous retroviral element, now called HERV-K-T47D, to the HML-4 subgroup of the HERV-K superfamily. The gag, prt, pol, and env genes exhibit 40 to 60% amino acid identity to HERV-K10. HERV-K-T47D is located on human chromosome 10, with five closely related elements on chromosomes 8, 9, 15, 16, and 19 and several hundred HERV-K-T47D-related solitary LTRs dispersed over the human genome. HERV-K-T47D-related sequences are detected in the genomes of higher primates and Old World monkeys but not in those of New World monkeys. High HERV-K-T47D transcription levels were observed in human placenta tissue, whereas transcription in T47D cells was strictly steroid dependent.

* Corresponding author. Mailing address: III Medizinische Klinik, Klinikum Mannheim der Universität Heidelberg, Wiesbadener Strasse 7-11, D-68305 Mannheim, Germany. Phone: (49) 621 383-4103. Fax: (49) 621 383-4201. E-mail: seifarth{at}rumms.uni-mannheim.de.

Journal of Virology, October 1998, p. 8384-8391, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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