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Journal of Virology, October 1998, p. 8316-8320, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

In Vivo Translation of the Triple Gene Block of Potato Virus X Requires Two Subgenomic mRNAs

Jeanmarie Verchot,dagger Susan M. Angell, and David C. Baulcombe*

Sainsbury Laboratory, Norwich Research Park, Colney, United Kingdom NR4 7UH

Received 9 March 1998/Accepted 2 June 1998

The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.

* Corresponding author. Mailing address: The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney, United Kingdom NR4 7UH. Phone: 44-1603-452571. Fax: 44-1603-250024. E-mail: baulcombe{at}bbsrc.ac.uk.

dagger Present address: Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078.

Journal of Virology, October 1998, p. 8316-8320, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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