This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyoshi, H.
Right arrow Articles by Verma, I. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyoshi, H.
Right arrow Articles by Verma, I. M.

 Previous Article  |  Next Article 

Journal of Virology, October 1998, p. 8150-8157, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of a Self-Inactivating Lentivirus Vector

Hiroyuki Miyoshi, Ulrike Blömer,dagger Masayo Takahashi,Dagger Fred H. Gage, and Inder M. Verma*

Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037

Received 30 April 1998/Accepted 13 July 1998

We have constructed a new series of lentivirus vectors based on human immunodeficiency virus type 1 (HIV-1) that can transduce nondividing cells. The U3 region of the 5' long terminal repeat (LTR) in vector constructs was replaced with the cytomegalovirus (CMV) promoter, resulting in Tat-independent transcription but still maintaining high levels of expression. A self-inactivating (SIN) vector was constructed by deleting 133 bp in the U3 region of the 3' LTR, including the TATA box and binding sites for transcription factors Sp1 and NF-kappa B. The deletion is transferred to the 5' LTR after reverse transcription and integration in infected cells, resulting in the transcriptional inactivation of the LTR in the proviruses. SIN viruses can be generated with no significant decreases in titer. Injection of viruses into the rat brain showed that a SIN vector containing the green fluorescent protein gene under the control of the internal CMV promoter transduced neurons as efficiently as a wild-type vector. Interestingly, a wild-type vector without an internal promoter also successfully transduced neurons in the brain, indicating that the HIV-1 LTR promoter is transcriptionally active in neurons even in the absence of Tat. Furthermore, injection of viruses into the subretinal space of the rat eye showed that wild-type vector transduced predominantly retinal pigment epithelium and photoreceptor cells, while SIN vector was able to transduce other types of retinal cells, including bipolar, Müller, horizontal, and amacrine cells. This finding suggests that the HIV-1 LTR can negatively influence the internal CMV promoter in some cell types. SIN HIV vectors should be safer for gene therapy, and they also have broader applicability as a means of high-level gene transfer and expression in nondividing cells.

* Corresponding author. Mailing address: Laboratory of Genetics, The Salk Institute for Biological Studies, P.O. Box 85800, San Diego, CA 92186-5800. Phone: (619) 453-4100, ext. 1462. Fax: (619) 558-7454. E-mail: verma{at}salk.edu.

dagger Present address: Department for Neurosurgery, Medical School Hannover, 30625 Hannover, Germany.

Dagger Present address: Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan.

Journal of Virology, October 1998, p. 8150-8157, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yamaguchi, S., Kurimoto, K., Yabuta, Y., Sasaki, H., Nakatsuji, N., Saitou, M., Tada, T. (2009). Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells. Development 136: 4011-4020 [Abstract] [Full Text]  
  • Karanikolas, B. D.W., Figueiredo, M. L., Wu, L. (2009). Polycomb Group Protein Enhancer of Zeste 2 Is an Oncogene That Promotes the Neoplastic Transformation of a Benign Prostatic Epithelial Cell Line. Mol Cancer Res 7: 1456-1465 [Abstract] [Full Text]  
  • Takashima, S., Takehashi, M., Lee, J., Chuma, S., Okano, M., Hata, K., Suetake, I., Nakatsuji, N., Miyoshi, H., Tajima, S., Tanaka, Y., Toyokuni, S., Sasaki, H., Kanatsu-Shinohara, M., Shinohara, T. (2009). Abnormal DNA Methyltransferase Expression in Mouse Germline Stem Cells Results in Spermatogenic Defects. Biol. Reprod. 81: 155-164 [Abstract] [Full Text]  
  • Sadagopan, S., Sharma-Walia, N., Veettil, M. V., Bottero, V., Levine, R., Vart, R. J., Chandran, B. (2009). Kaposi's Sarcoma-Associated Herpesvirus Upregulates Angiogenin during Infection of Human Dermal Microvascular Endothelial Cells, Which Induces 45S rRNA Synthesis, Antiapoptosis, Cell Proliferation, Migration, and Angiogenesis. J. Virol. 83: 3342-3364 [Abstract] [Full Text]  
  • Morishita, H., Saito, F., Kayama, H., Atarashi, K., Kuwata, H., Yamamoto, M., Takeda, K. (2009). Fra-1 negatively regulates lipopolysaccharide-mediated inflammatory responses. Int Immunol 21: 457-465 [Abstract] [Full Text]  
  • Sasaki, R., Narisawa-Saito, M., Yugawa, T., Fujita, M., Tashiro, H., Katabuchi, H., Kiyono, T. (2009). Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis 30: 423-431 [Abstract] [Full Text]  
  • Akamatsu, W., DeVeale, B., Okano, H., Cooney, A. J, van der Kooy, D. (2009). Suppression of Oct4 by Germ Cell Nuclear Factor Restricts Pluripotency and Promotes Neural Stem Cell Development in the Early Neural Lineage. J. Neurosci. 29: 2113-2124 [Abstract] [Full Text]  
  • Matsuda, S., Mikami, Y., Ohtani, M., Fujiwara, M., Hirata, Y., Minowa, A., Terauchi, Y., Kadowaki, T., Koyasu, S. (2009). Critical role of class IA PI3K for c-Rel expression in B lymphocytes. Blood 113: 1037-1044 [Abstract] [Full Text]  
  • Imai, K., Hirata, S., Irie, A., Senju, S., Ikuta, Y., Yokomine, K., Harao, M., Inoue, M., Tsunoda, T., Nakatsuru, S., Nakagawa, H., Nakamura, Y., Baba, H., Nishimura, Y. (2008). Identification of a Novel Tumor-Associated Antigen, Cadherin 3/P-Cadherin, as a Possible Target for Immunotherapy of Pancreatic, Gastric, and Colorectal Cancers. Clin. Cancer Res. 14: 6487-6495 [Abstract] [Full Text]  
  • Ohtani, M., Nagai, S., Kondo, S., Mizuno, S., Nakamura, K., Tanabe, M., Takeuchi, T., Matsuda, S., Koyasu, S. (2008). Mammalian target of rapamycin and glycogen synthase kinase 3 differentially regulate lipopolysaccharide-induced interleukin-12 production in dendritic cells. Blood 112: 635-643 [Abstract] [Full Text]  
  • Monfared, P., Winkeler, A., Klein, M., Li, H., Klose, A., Hoesel, M., Waerzeggers, Y., Korsching, S., Jacobs, A. H. (2008). Noninvasive Assessment of E2F-1-Mediated Transcriptional Regulation In vivo. Cancer Res. 68: 5932-5940 [Abstract] [Full Text]  
  • Yamashita, M., Kuwahara, M., Suzuki, A., Hirahara, K., Shinnaksu, R., Hosokawa, H., Hasegawa, A., Motohashi, S., Iwama, A., Nakayama, T. (2008). Bmi1 regulates memory CD4 T cell survival via repression of the Noxa gene. JEM 205: 1109-1120 [Abstract] [Full Text]  
  • El-Amouri, S. S., Zhu, H., Yu, J., Marr, R., Verma, I. M., Kindy, M. S. (2008). Neprilysin: An Enzyme Candidate to Slow the Progression of Alzheimer's Disease. Am. J. Pathol. 172: 1342-1354 [Abstract] [Full Text]  
  • Shah, K., Hingtgen, S., Kasmieh, R., Figueiredo, J. L., Garcia-Garcia, E., Martinez-Serrano, A., Breakefield, X., Weissleder, R. (2008). Bimodal Viral Vectors and In Vivo Imaging Reveal the Fate of Human Neural Stem Cells in Experimental Glioma Model. J. Neurosci. 28: 4406-4413 [Abstract] [Full Text]  
  • Roberts, T. F., Klein, M. E., Kubke, M. F., Wild, J. M., Mooney, R. (2008). Telencephalic Neurons Monosynaptically Link Brainstem and Forebrain Premotor Networks Necessary for Song. J. Neurosci. 28: 3479-3489 [Abstract] [Full Text]  
  • Schumacher, A. J., Hache, G., MacDuff, D. A., Brown, W. L., Harris, R. S. (2008). The DNA Deaminase Activity of Human APOBEC3G Is Required for Ty1, MusD, and Human Immunodeficiency Virus Type 1 Restriction. J. Virol. 82: 2652-2660 [Abstract] [Full Text]  
  • Li, W., Xie, S., Guo, X., Gong, X., Wang, S., Lin, D., Zhang, J., Ren, Z., Huang, S., Zeng, F., Zeng, Y. (2008). A novel transgenic mouse model produced from lentiviral germline integration for the study of {beta}-thalassemia gene therapy. haematol 93: 356-362 [Abstract] [Full Text]  
  • Akter, Mst. H., Chano, T., Okabe, H., Yamaguchi, T., Hirose, F., Osumi, T. (2008). Target Specificities of Estrogen Receptor-Related Receptors: Analysis of Binding Sequences and Identification of Rb1-Inducible Coiled-Coil 1 (Rb1cc1) as a Target Gene. J Biochem 143: 395-406 [Abstract] [Full Text]  
  • Ryu, B. Y., Evans-Galea, M. V., Gray, J. T., Bodine, D. M., Persons, D. A., Nienhuis, A. W. (2008). An experimental system for the evaluation of retroviral vector design to diminish the risk for proto-oncogene activation. Blood 111: 1866-1875 [Abstract] [Full Text]  
  • Braiterman, L. T., Heffernan, S., Nyasae, L., Johns, D., See, A. P., Yutzy, R., McNickle, A., Herman, M., Sharma, A., Naik, U. P., Hubbard, A. L. (2008). JAM-A is both essential and inhibitory to development of hepatic polarity in WIF-B cells. Am. J. Physiol. Gastrointest. Liver Physiol. 294: G576-G588 [Abstract] [Full Text]  
  • Cheng, J. C., Kinjo, K., Judelson, D. R., Chang, J., Wu, W. S., Schmid, I., Shankar, D. B., Kasahara, N., Stripecke, R., Bhatia, R., Landaw, E. M., Sakamoto, K. M. (2008). CREB is a critical regulator of normal hematopoiesis and leukemogenesis. Blood 111: 1182-1192 [Abstract] [Full Text]  
  • Ruiz-Vela, A., Aggarwal, M., de la Cueva, P., Treda, C., Herreros, B., Martin-Perez, D., Dominguez, O., Piris, M. A. (2008). Lentiviral (HIV)-based RNA interference screen in human B-cell receptor regulatory networks reveals MCL1-induced oncogenic pathways. Blood 111: 1665-1676 [Abstract] [Full Text]  
  • Shani, G., Fischer, W. H., Justice, N. J., Kelber, J. A., Vale, W., Gray, P. C. (2008). GRP78 and Cripto Form a Complex at the Cell Surface and Collaborate To Inhibit Transforming Growth Factor Signaling and Enhance Cell Growth. Mol. Cell. Biol. 28: 666-677 [Abstract] [Full Text]  
  • Gunawardena, S. R., Ruis, B. L., Meyer, J. A., Kapoor, M., Conklin, K. F. (2008). NOM1 Targets Protein Phosphatase I to the Nucleolus. J. Biol. Chem. 283: 398-404 [Abstract] [Full Text]  
  • Lyon, A R, Sato, M, Hajjar, R J, Samulski, R J, Harding, S E (2008). Gene therapy: targeting the myocardium. Heart 94: 89-99 [Abstract] [Full Text]  
  • Kaminuma, O., Kitamura, F., Kitamura, N., Hiroi, T., Miyoshi, H., Miyawaki, A., Miyatake, S. (2008). Differential Contribution of NFATc2 and NFATc1 to TNF-{alpha} Gene Expression in T Cells. J. Immunol. 180: 319-326 [Abstract] [Full Text]  
  • Hendrickson, B., Senadheera, D., Mishra, S., Bui, K. C. T., Wang, X., Chan, B., Petersen, D., Pepper, K., Lutzko, C. (2007). Development of Lentiviral Vectors with Regulated Respiratory Epithelial Expression In Vivo. Am. J. Respir. Cell Mol. Bio. 37: 414-423 [Abstract] [Full Text]  
  • Potikyan, G., Savene, R. O.V., Gaulden, J. M., France, K. A., Zhou, Z., Kleinerman, E. S., Lessnick, S. L., Denny, C. T. (2007). EWS/FLI1 Regulates Tumor Angiogenesis in Ewing's Sarcoma via Suppression of Thrombospondins. Cancer Res. 67: 6675-6684 [Abstract] [Full Text]  
  • Bonn, S., Seeburg, P. H., Schwarz, M. K. (2007). Combinatorial Expression of {alpha}- and {gamma}-Protocadherins Alters Their Presenilin-Dependent Processing. Mol. Cell. Biol. 27: 4121-4132 [Abstract] [Full Text]  
  • Uesaka, T., Jain, S., Yonemura, S., Uchiyama, Y., Milbrandt, J., Enomoto, H. (2007). Conditional ablation of GFR{alpha}1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. Development 134: 2171-2181 [Abstract] [Full Text]  
  • Cui, C.-H., Uyama, T., Miyado, K., Terai, M., Kyo, S., Kiyono, T., Umezawa, A. (2007). Menstrual Blood-derived Cells Confer Human Dystrophin Expression in the Murine Model of Duchenne Muscular Dystrophy via Cell Fusion and Myogenic Transdifferentiation. Mol. Biol. Cell 18: 1586-1594 [Abstract] [Full Text]  
  • Greenberg, K. P., Geller, S. F., Schaffer, D. V., Flannery, J. G. (2007). Targeted Transgene Expression in Muller Glia of Normal and Diseased Retinas Using Lentiviral Vectors. IOVS 48: 1844-1852 [Abstract] [Full Text]  
  • Masuda, H., Maruyama, T., Hiratsu, E., Yamane, J., Iwanami, A., Nagashima, T., Ono, M., Miyoshi, H., Okano, H. J., Ito, M., Tamaoki, N., Nomura, T., Okano, H., Matsuzaki, Y., Yoshimura, Y. (2007). Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD/SCID/{gamma}Formula immunodeficient mice. Proc. Natl. Acad. Sci. USA 104: 1925-1930 [Abstract] [Full Text]  
  • Whitcomb, J. M., Huang, W., Fransen, S., Limoli, K., Toma, J., Wrin, T., Chappey, C., Kiss, L. D. B., Paxinos, E. E., Petropoulos, C. J. (2007). Development and Characterization of a Novel Single-Cycle Recombinant-Virus Assay To Determine Human Immunodeficiency Virus Type 1 Coreceptor Tropism. Antimicrob. Agents Chemother. 51: 566-575 [Abstract] [Full Text]  
  • Gray, P. C., Shani, G., Aung, K., Kelber, J., Vale, W. (2006). Cripto Binds Transforming Growth Factor {beta} (TGF-{beta}) and Inhibits TGF-{beta} Signaling. Mol. Cell. Biol. 26: 9268-9278 [Abstract] [Full Text]  
  • Vogel, M. J., Guelen, L., de Wit, E., Hupkes, D. P., Loden, M., Talhout, W., Feenstra, M., Abbas, B., Classen, A.-K., van Steensel, B. (2006). Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Genome Res 16: 1493-1504 [Abstract] [Full Text]  
  • Irie, A., Harada, K., Tsukamoto, H., Kim, J.-R., Araki, N., Nishimura, Y. (2006). Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int Immunol 18: 1737-1747 [Abstract] [Full Text]  
  • Philippe, S., Sarkis, C., Barkats, M., Mammeri, H., Ladroue, C., Petit, C., Mallet, J., Serguera, C. (2006). Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo. Proc. Natl. Acad. Sci. USA 103: 17684-17689 [Abstract] [Full Text]  
  • Yamamoto, T., Miyoshi, H., Yamamoto, N., Yamamoto, N., Inoue, J.-i., Tsunetsugu-Yokota, Y. (2006). Lentivirus vectors expressing short hairpin RNAs against the U3-overlapping region of HIV nef inhibit HIV replication and infectivity in primary macrophages. Blood 108: 3305-3312 [Abstract] [Full Text]  
  • Jonsson, S. R., Hache, G., Stenglein, M. D., Fahrenkrug, S. C., Andresdottir, V., Harris, R. S. (2006). Evolutionarily conserved and non-conserved retrovirus restriction activities of artiodactyl APOBEC3F proteins. Nucleic Acids Res 34: 5683-5694 [Abstract] [Full Text]  
  • Mortellaro, A., Hernandez, R. J., Guerrini, M. M., Carlucci, F., Tabucchi, A., Ponzoni, M., Sanvito, F., Doglioni, C., Serio, C. D., Biasco, L., Follenzi, A., Naldini, L., Bordignon, C., Roncarolo, M. G., Aiuti, A. (2006). Ex vivo gene therapy with lentiviral vectors rescues adenosine deaminase (ADA)-deficient mice and corrects their immune and metabolic defects. Blood 108: 2979-2988 [Abstract] [Full Text]  
  • Monse, H., Laufs, S., Kuate, S., Zeller, W. J., Fruehauf, S., Uberla, K. (2006). Viral determinants of integration site preferences of simian immunodeficiency virus-based vectors.. J. Virol. 80: 8145-8150 [Abstract] [Full Text]  
  • Hamamoto, S., Nishitsuji, H., Amagasa, T., Kannagi, M., Masuda, T. (2006). Identification of a Novel Human Immunodeficiency Virus Type 1 Integrase Interactor, Gemin2, That Facilitates Efficient Viral cDNA Synthesis In Vivo.. J. Virol. 80: 5670-5677 [Abstract] [Full Text]  
  • Ando, K., Yahata, T., Sato, T., Miyatake, H., Matsuzawa, H., Oki, M., Miyoshi, H., Tsuji, T., Kato, S., Hotta, T. (2006). Direct evidence for ex vivo expansion of human hematopoietic stem cells. Blood 107: 3371-3377 [Abstract] [Full Text]  
  • Yu, J. H., Schaffer, D. V. (2006). Selection of novel vesicular stomatitis virus glycoprotein variants from a Peptide insertion library for enhanced purification of retroviral and lentiviral vectors.. J. Virol. 80: 3285-3292 [Abstract] [Full Text]  
  • Wu, J. C., Spin, J. M., Cao, F., Lin, S., Xie, X., Gheysens, O., Chen, I. Y., Sheikh, A. Y., Robbins, R. C., Tsalenko, A., Gambhir, S. S., Quertermous, T. (2006). Transcriptional profiling of reporter genes used for molecular imaging of embryonic stem cell transplantation. Physiol. Genomics 25: 29-38 [Abstract] [Full Text]  
  • Muotri, A. R., Nakashima, K., Toni, N., Sandler, V. M., Gage, F. H. (2005). Development of functional human embryonic stem cell-derived neurons in mouse brain. Proc. Natl. Acad. Sci. USA 102: 18644-18648 [Abstract] [Full Text]  
  • Sugiyama, S.-i., Shimada, N., Miyoshi, H., Yamauchi, K. (2005). Detection of Thyroid System-Disrupting Chemicals Using in Vitro and in Vivo Screening Assays in Xenopus laevis. Toxicol Sci 88: 367-374 [Abstract] [Full Text]  
  • Scott, B. B., Lois, C. (2005). Generation of tissue-specific transgenic birds with lentiviral vectors. Proc. Natl. Acad. Sci. USA 102: 16443-16447 [Abstract] [Full Text]  
  • Li, Q., Lu, Q., Estepa, G., Verma, I. M. (2005). Identification of 14-3-3{sigma} mutation causing cutaneous abnormality in repeated-epilation mutant mouse. Proc. Natl. Acad. Sci. USA 102: 15977-15982 [Abstract] [Full Text]  
  • Li, Q., Lu, Q., Bottero, V., Estepa, G., Morrison, L., Mercurio, F., Verma, I. M. (2005). Enhanced NF-{kappa}B activation and cellular function in macrophages lacking I{kappa}B kinase 1 (IKK1). Proc. Natl. Acad. Sci. USA 102: 12425-12430 [Abstract] [Full Text]  
  • Ikeda, H., Osakada, F., Watanabe, K., Mizuseki, K., Haraguchi, T., Miyoshi, H., Kamiya, D., Honda, Y., Sasai, N., Yoshimura, N., Takahashi, M., Sasai, Y. (2005). Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells. Proc. Natl. Acad. Sci. USA 102: 11331-11336 [Abstract] [Full Text]  
  • Nakayama, E. E., Miyoshi, H., Nagai, Y., Shioda, T. (2005). A Specific Region of 37 Amino Acid Residues in the SPRY (B30.2) Domain of African Green Monkey TRIM5{alpha} Determines Species-Specific Restriction of Simian Immunodeficiency Virus SIVmac Infection. J. Virol. 79: 8870-8877 [Abstract] [Full Text]  
  • Lucke, S., Grunwald, T., Uberla, K. (2005). Reduced Mobilization of Rev-Responsive Element-Deficient Lentiviral Vectors. J. Virol. 79: 9359-9362 [Abstract] [Full Text]  
  • Hanawa, H., Persons, D. A., Nienhuis, A. W. (2005). Mobilization and Mechanism of Transcription of Integrated Self-Inactivating Lentiviral Vectors. J. Virol. 79: 8410-8421 [Abstract] [Full Text]  
  • Hache, G., Liddament, M. T., Harris, R. S. (2005). The Retroviral Hypermutation Specificity of APOBEC3F and APOBEC3G Is Governed by the C-terminal DNA Cytosine Deaminase Domain. J. Biol. Chem. 280: 10920-10924 [Abstract] [Full Text]  
  • Kojima, H., Sasaki, T., Ishitani, T., Iemura, S.-i., Zhao, H., Kaneko, S., Kunimoto, H., Natsume, T., Matsumoto, K., Nakajima, K. (2005). STAT3 regulates Nemo-like kinase by mediating its interaction with IL-6-stimulated TGF{beta}-activated kinase 1 for STAT3 Ser-727 phosphorylation. Proc. Natl. Acad. Sci. USA 102: 4524-4529 [Abstract] [Full Text]  
  • Hirotani, T., Lee, P. Y., Kuwata, H., Yamamoto, M., Matsumoto, M., Kawase, I., Akira, S., Takeda, K. (2005). The Nuclear I{kappa}B Protein I{kappa}BNS Selectively Inhibits Lipopolysaccharide-Induced IL-6 Production in Macrophages of the Colonic Lamina Propria. J. Immunol. 174: 3650-3657 [Abstract] [Full Text]  
  • Motoyama, M., Yamazaki, S., Eto-Kimura, A., Takeshige, K., Muta, T. (2005). Positive and Negative Regulation of Nuclear Factor-{kappa}B-mediated Transcription by I{kappa}B-{zeta}, an Inducible Nuclear Protein. J. Biol. Chem. 280: 7444-7451 [Abstract] [Full Text]  
  • Rivera, S., Liu, L., Nemeth, E., Gabayan, V., Sorensen, O. E., Ganz, T. (2005). Hepcidin excess induces the sequestration of iron and exacerbates tumor-associated anemia. Blood 105: 1797-1802 [Abstract] [Full Text]  
  • Dodart, J.-C., Marr, R. A., Koistinaho, M., Gregersen, B. M., Malkani, S., Verma, I. M., Paul, S. M. (2005). Gene delivery of human apolipoprotein E alters brain A{beta} burden in a mouse model of Alzheimer's disease. Proc. Natl. Acad. Sci. USA 102: 1211-1216 [Abstract] [Full Text]  
  • Malik, P., Arumugam, P. I. (2005). Gene Therapy for {beta}-Thalassemia. ASH Education Book 2005: 45-50 [Abstract] [Full Text]  
  • Dittgen, T., Nimmerjahn, A., Komai, S., Licznerski, P., Waters, J., Margrie, T. W., Helmchen, F., Denk, W., Brecht, M., Osten, P. (2004). Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proc. Natl. Acad. Sci. USA 101: 18206-18211 [Abstract] [Full Text]  
  • Coles, B. L. K., Angenieux, B., Inoue, T., Del Rio-Tsonis, K., Spence, J. R., McInnes, R. R., Arsenijevic, Y., van der Kooy, D. (2004). Facile isolation and the characterization of human retinal stem cells. Proc. Natl. Acad. Sci. USA 101: 15772-15777 [Abstract] [Full Text]  
  • Yamashita, D., Yamaguchi, T., Shimizu, M., Nakata, N., Hirose, F., Osumi, T. (2004). The transactivating function of peroxisome proliferator-activated receptor {gamma} is negatively regulated by SUMO conjugation in the amino-terminal domain. GENES CELLS 9: 1017-1029 [Abstract] [Full Text]  
  • Logan, A. C., Haas, D. L., Kafri, T., Kohn, D. B. (2004). Integrated Self-Inactivating Lentiviral Vectors Produce Full-Length Genomic Transcripts Competent for Encapsidation and Integration. J. Virol. 78: 8421-8436 [Abstract] [Full Text]  
  • Hofmann, A., Zakhartchenko, V., Weppert, M., Sebald, H., Wenigerkind, H., Brem, G., Wolf, E., Pfeifer, A. (2004). Generation of Transgenic Cattle by Lentiviral Gene Transfer into Oocytes. Biol. Reprod. 71: 405-409 [Abstract] [Full Text]  
  • Mitta, B., Weber, C. C., Rimann, M., Fussenegger, M. (2004). Design and in vivo characterization of self-inactivating human and non-human lentiviral expression vectors engineered for streptogramin-adjustable transgene expression. Nucleic Acids Res 32: e106-e106 [Abstract] [Full Text]  
  • Dai, C., McAninch, R. E., Sutton, R. E. (2004). Identification of Synthetic Endothelial Cell-Specific Promoters by Use of a High-Throughput Screen. J. Virol. 78: 6209-6221 [Abstract] [Full Text]  
  • Miyagi, S., Saito, T., Mizutani, K.-i., Masuyama, N., Gotoh, Y., Iwama, A., Nakauchi, H., Masui, S., Niwa, H., Nishimoto, M., Muramatsu, M., Okuda, A. (2004). The Sox-2 Regulatory Regions Display Their Activities in Two Distinct Types of Multipotent Stem Cells. Mol. Cell. Biol. 24: 4207-4220 [Abstract] [Full Text]  
  • Breckpot, K., Heirman, C., De Greef, C., van der Bruggen, P., Thielemans, K. (2004). Identification of New Antigenic Peptide Presented by HLA-Cw7 and Encoded by Several MAGE Genes Using Dendritic Cells Transduced with Lentiviruses. J. Immunol. 172: 2232-2237 [Abstract] [Full Text]  
  • Barzon, L., Boscaro, M., Palu, G. (2004). Endocrine Aspects of Cancer Gene Therapy. Endocr. Rev. 25: 1-44 [Abstract] [Full Text]  
  • Shibuya, K., Shirakawa, J., Kameyama, T., Honda, S.-i., Tahara-Hanaoka, S., Miyamoto, A., Onodera, M., Sumida, T., Nakauchi, H., Miyoshi, H., Shibuya, A. (2003). CD226 (DNAM-1) Is Involved in Lymphocyte Function-associated Antigen 1 Costimulatory Signal for Naive T Cell Differentiation and Proliferation. JEM 198: 1829-1839 [Abstract] [Full Text]  
  • Kuwata, H., Watanabe, Y., Miyoshi, H., Yamamoto, M., Kaisho, T., Takeda, K., Akira, S. (2003). IL-10-inducible Bcl-3 negatively regulates LPS-induced TNF-{alpha} production in macrophages. Blood 102: 4123-4129 [Abstract] [Full Text]  
  • Tschan, M. P., Fischer, K. M., Fung, V. S., Pirnia, F., Borner, M. M., Fey, M. F., Tobler, A., Torbett, B. E. (2003). Alternative Splicing of the Human Cyclin D-binding Myb-like Protein (hDMP1) Yields a Truncated Protein Isoform That Alters Macrophage Differentiation Patterns. J. Biol. Chem. 278: 42750-42760 [Abstract] [Full Text]  
  • Haas, D. L., Lutzko, C., Logan, A. C., Cho, G. J., Skelton, D., Jin Yu, X., Pepper, K. A., Kohn, D. B. (2003). The Moloney Murine Leukemia Virus Repressor Binding Site Represses Expression in Murine and Human Hematopoietic Stem Cells. J. Virol. 77: 9439-9450 [Abstract] [Full Text]  
  • Lutzko, C., Senadheera, D., Skelton, D., Petersen, D., Kohn, D. B. (2003). Lentivirus Vectors Incorporating the Immunoglobulin Heavy Chain Enhancer and Matrix Attachment Regions Provide Position-Independent Expression in B Lymphocytes. J. Virol. 77: 7341-7351 [Abstract] [Full Text]  
  • Dirac, A. M. G., Bernards, R. (2003). Reversal of Senescence in Mouse Fibroblasts through Lentiviral Suppression of p53. J. Biol. Chem. 278: 11731-11734 [Abstract] [Full Text]  
  • Marr, R. A., Rockenstein, E., Mukherjee, A., Kindy, M. S., Hersh, L. B., Gage, F. H., Verma, I. M., Masliah, E. (2003). Neprilysin Gene Transfer Reduces Human Amyloid Pathology in Transgenic Mice. J. Neurosci. 23: 1992-1996 [Abstract] [Full Text]  
  • Kubo, S., Mitani, K. (2003). A New Hybrid System Capable of Efficient Lentiviral Vector Production and Stable Gene Transfer Mediated by a Single Helper-Dependent Adenoviral Vector. J. Virol. 77: 2964-2971 [Abstract] [Full Text]  
  • Roszkowski, J. J., Yu, D. C., Rubinstein, M. P., McKee, M. D., Cole, D. J., Nishimura, M. I. (2003). CD8-Independent Tumor Cell Recognition Is a Property of the T Cell Receptor and Not the T Cell. J. Immunol. 170: 2582-2589 [Abstract] [Full Text]  
  • Xia, Y., Pao, G. M., Chen, H.-W., Verma, I. M., Hunter, T. (2003). Enhancement of BRCA1 E3 Ubiquitin Ligase Activity through Direct Interaction with the BARD1 Protein. J. Biol. Chem. 278: 5255-5263 [Abstract] [Full Text]  
  • Coleman, J. E., Huentelman, M. J., Kasparov, S., Metcalfe, B. L., Paton, J. F. R., Katovich, M. J., Semple-Rowland, S. L., Raizada, M. K. (2003). Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Physiol. Genomics 12: 221-228 [Abstract] [Full Text]  
  • Gough, P. J., Raines, E. W. (2003). Gene therapy of apolipoprotein E-deficient mice using a novel macrophage-specific retroviral vector. Blood 101: 485-491 [Abstract] [Full Text]  
  • Mitta, B., Rimann, M., Ehrengruber, M. U., Ehrbar, M., Djonov, V., Kelm, J., Fussenegger, M. (2002). Advanced modular self-inactivating lentiviral expression vectors for multigene interventions in mammalian cells and in vivo transduction. Nucleic Acids Res 30: e113-e113 [Abstract] [Full Text]  
  • Lai, Z., Han, I., Park, M., Brady, R. O. (2002). Design of an HIV-1 lentiviral-based gene-trap vector to detect developmentally regulated genes in mammalian cells. Proc. Natl. Acad. Sci. USA 99: 3651-3656 [Abstract] [Full Text]  
  • Pfeifer, A., Ikawa, M., Dayn, Y., Verma, I. M. (2002). Transgenesis by lentiviral vectors: Lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc. Natl. Acad. Sci. USA 99: 2140-2145 [Abstract] [Full Text]  
  • Konetschny, C., Holzer, G. W., Falkner, F. G. (2002). Retroviral Vectors Produced in the Cytoplasmic Vaccinia Virus System Transduce Intron-Containing Genes. J. Virol. 76: 1236-1243 [Abstract] [Full Text]  
  • Cui, Y., Golob, J., Kelleher, E., Ye, Z., Pardoll, D., Cheng, L. (2002). Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells. Blood 99: 399-408 [Abstract] [Full Text]  
  • Borok, Z., Harboe-Schmidt, J. E., Brody, S. L., You, Y., Zhou, B., Li, X., Cannon, P. M., Kim, K.-J., Crandall, E. D., Kasahara, N. (2001). Vesicular Stomatitis Virus G-Pseudotyped Lentivirus Vectors Mediate Efficient Apical Transduction of Polarized Quiescent Primary Alveolar Epithelial Cells. J. Virol. 75: 11747-11754 [Abstract] [Full Text]  
  • Pang, S., Kang, M. K., Kung, S., Yu, D., Lee, A., Poon, B., Chen, I. S. Y., Lindemann, B., Park, N.-H. (2001). Anticancer Effect of a Lentiviral Vector Capable of Expressing HIV-1 Vpr. Clin. Cancer Res. 7: 3567-3573 [Abstract] [Full Text]  
  • Moreau-Gaudry, F., Xia, P., Jiang, G., Perelman, N. P., Bauer, G., Ellis, J., Surinya, K. H., Mavilio, F., Shen, C.-K., Malik, P. (2001). High-level erythroid-specific gene expression in primary human and murine hematopoietic cells with self-inactivating lentiviral vectors. Blood 98: 2664-2672 [Abstract] [Full Text]  
  • Lewis, B. C., Chinnasamy, N., Morgan, R. A., Varmus, H. E. (2001). Development of an Avian Leukosis-Sarcoma Virus Subgroup A Pseudotyped Lentiviral Vector. J. Virol. 75: 9339-9344 [Abstract] [Full Text]  
  • Wolfgang, M. J., Eisele, S. G., Browne, M. A., Schotzko, M. L., Garthwaite, M. A., Durning, M., Ramezani, A., Hawley, R. G., Thomson, J. A., Golos, T. G. (2001). Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos. Proc. Natl. Acad. Sci. USA 98: 10728-10732 [Abstract] [Full Text]  
  • Pfeifer, A., Brandon, E. P., Kootstra, N., Gage, F. H., Verma, I. M. (2001). Delivery of the Cre recombinase by a self-deleting lentiviral vector: Efficient gene targeting in vivo. Proc. Natl. Acad. Sci. USA 10.1073/pnas.201415498v1 [Abstract] [Full Text]  
  • Kowolik, C. M., Hu, J., Yee, J.-K. (2001). Locus Control Region of the Human CD2 Gene in a Lentivirus Vector Confers Position-Independent Transgene Expression. J. Virol. 75: 4641-4648 [Abstract] [Full Text]  
  • Berkowitz, R., Ilves, H., Lin, W. Y., Eckert, K., Coward, A., Tamaki, S., Veres, G., Plavec, I. (2001). Construction and Molecular Analysis of Gene Transfer Systems Derived from Bovine Immunodeficiency Virus. J. Virol. 75: 3371-3382 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»