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J Virol, January 1998, p. 853-856, Vol. 72, No. 1
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Moloney Murine Leukemia Virus-Based Retroviral Vector Pseudotyped by the Insect Retroviral gypsy Envelope Can Infect Drosophila Cells

Laure Teysset,1 Jane C. Burns,2 Hiroko Shike,2 Barbara L. Sullivan,2 Alain Bucheton,1 and Christophe Terzian1,*

Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France,1 and Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, California2

Received 27 March 1997/Accepted 14 October 1997

The gypsy element of Drosophila melanogaster is the first retrovirus identified so far in invertebrates. Previous data suggest that gypsy ENV-like ORF3 mediates viral infectivity. We have produced in the 293GP/LNhsp70lucL.3 human cell line a Moloney murine leukemia virus-based retroviral vector pseudotyped by the gypsy ENV-like protein. We have shown by immunostaining that the gypsy envelope protein is produced in 293GP/LNhsp70lucL.3 cells and that vector particles collected from these cells can infect Drosophila cells. Our results provide direct evidence that the infectious property of gypsy is due to its ORF3 gene product.

* Corresponding author. Mailing address: Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette Cedex, France. Phone: 33-1 69 82 31 46. Fax: 33-1 69 82 43 86. E-mail: terzian{at}cgm.cnrs-gif.fr.




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