This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dumonceaux, J.
Right arrow Articles by Hazan, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dumonceaux, J.
Right arrow Articles by Hazan, U.

 Previous Article  |  Next Article 

J. Virol., Jan 1998, 512-519, Vol 72, No. 1
Copyright © 1998, American Society for Microbiology

Spontaneous mutations in the env gene of the human immunodeficiency virus type 1 NDK isolate are associated with a CD4-independent entry phenotype

J Dumonceaux, S Nisole, C Chanel, L Quivet, A Amara, F Baleux, P Briand and U Hazan
INSERM Unite 380 Laboratoire de Pathologie et Genetique Experimentales, Institut Cochin de Genetique Moleculaire, Paris, France.

Human immunodeficiency virus type 1 (HIV-1) entry into target cells is a multistep process initiated by envelope protein gp120 binding to cell surface CD4. The conformational changes induced by this interaction likely favor a second-step interaction between gp120 and a coreceptor such as CXCR4 or CCR5. Here, we report a spontaneous and stable CD4- independent entry phenotype for the HIV-1 NDK isolate. This mutant strain, which emerged from a population of chronically infected CD4- positive CEM cells, can replicate in CD4-negative human cell lines. The presence of CXCR4 alone renders cells susceptible to infection by the mutant NDK, and infection can be blocked by the CXCR4 natural ligand SDF-1. Furthermore, we have correlated the CD4-independent phenotype with seven mutations in the C2 and C3 regions and the V3 loop. We propose that the mutant gp120 spontaneously acquires a conformation allowing it to interact directly with CXCR4. This virus provides us with a powerful tool to study directly gp120-CXCR4 interactions.


This article has been cited by other articles:

  • Perugi, F., Muriaux, D., Ramirez, B. C., Chabani, S., Decroly, E., Darlix, J.-L., Blot, V., Pique, C. (2009). Human Discs Large Is a New Negative Regulator of Human Immunodeficiency Virus-1 Infectivity. Mol. Biol. Cell 20: 498-508 [Abstract] [Full Text]  
  • Taylor, B. M., Foulke, J. S., Flinko, R., Heredia, A., DeVico, A., Reitz, M. (2008). An Alteration of Human Immunodeficiency Virus gp41 Leads to Reduced CCR5 Dependence and CD4 Independence. J. Virol. 82: 5460-5471 [Abstract] [Full Text]  
  • Ryu, B. Y., Zavorotinskaya, T., Trentin, B., Albritton, L. M. (2008). The block to membrane fusion differs with the site of ligand insertion in modified retroviral envelope proteins. J. Gen. Virol. 89: 1049-1058 [Abstract] [Full Text]  
  • Chien, M.-P., Jiang, S., Chang, D.-K. (2008). The function of coreceptor as a basis for the kinetic dissection of HIV type 1 envelope protein-mediated cell fusion. FASEB J. 22: 1179-1192 [Abstract] [Full Text]  
  • Kubo, Y., Yokoyama, M., Yoshii, H., Mitani, C., Tominaga, C., Tanaka, Y., Sato, H., Yamamoto, N. (2007). Inhibitory role of CXCR4 glycan in CD4-independent X4-tropic human immunodeficiency virus type 1 infection and its abrogation in CD4-dependent infection. J. Gen. Virol. 88: 3139-3144 [Abstract] [Full Text]  
  • Lin, G., Bertolotti-Ciarlet, A., Haggarty, B., Romano, J., Nolan, K. M., Leslie, G. J., Jordan, A. P.-O., Huang, C.-c., Kwong, P. D., Doms, R. W., Hoxie, J. A. (2007). Replication-Competent Variants of Human Immunodeficiency Virus Type 2 Lacking the V3 Loop Exhibit Resistance to Chemokine Receptor Antagonists. J. Virol. 81: 9956-9966 [Abstract] [Full Text]  
  • Waheed, A. A., Ablan, S. D., Mankowski, M. K., Cummins, J. E., Ptak, R. G., Schaffner, C. P., Freed, E. O. (2006). Inhibition of HIV-1 Replication by Amphotericin B Methyl Ester: SELECTION FOR RESISTANT VARIANTS. J. Biol. Chem. 281: 28699-28711 [Abstract] [Full Text]  
  • Wyss, S., Dimitrov, A. S., Baribaud, F., Edwards, T. G., Blumenthal, R., Hoxie, J. A. (2005). Regulation of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Fusion by a Membrane-Interactive Domain in the gp41 Cytoplasmic Tail. J. Virol. 79: 12231-12241 [Abstract] [Full Text]  
  • Zerhouni, B., Nelson, J. A. E., Saha, K. (2004). Isolation of CD4-Independent Primary Human Immunodeficiency Virus Type 1 Isolates That Are Syncytium Inducing and Acutely Cytopathic for CD8+ Lymphocytes. J. Virol. 78: 1243-1255 [Abstract] [Full Text]  
  • Vermeire, K., Schols, D. (2003). Specific CD4 down-modulating compounds with potent anti-HIV activity. J. Leukoc. Biol. 74: 667-675 [Abstract] [Full Text]  
  • McKnight, A., Weiss, R. A. (2003). Blocking the docking of HIV-1. Proc. Natl. Acad. Sci. USA 100: 10581-10582 [Full Text]  
  • Rainey, G. J. A., Natonson, A., Maxfield, L. F., Coffin, J. M. (2003). Mechanisms of Avian Retroviral Host Range Extension. J. Virol. 77: 6709-6719 [Abstract] [Full Text]  
  • Vermeire, K., Bell, T. W., Choi, H.-J., Jin, Q., Samala, M. F., Sodoma, A., De Clercq, E., Schols, D. (2003). The Anti-HIV Potency of Cyclotriazadisulfonamide Analogs Is Directly Correlated with Their Ability to Down-Modulate the CD4 Receptor. Mol. Pharmacol. 63: 203-210 [Abstract] [Full Text]  
  • Lin, G., Baribaud, F., Romano, J., Doms, R. W., Hoxie, J. A. (2002). Identification of gp120 Binding Sites on CXCR4 by Using CD4-Independent Human Immunodeficiency Virus Type 2 Env Proteins. J. Virol. 77: 931-942 [Abstract] [Full Text]  
  • Gallina, A., Hanley, T. M., Mandel, R., Trahey, M., Broder, C. C., Viglianti, G. A., Ryser, H. J.-P. (2002). Inhibitors of Protein-Disulfide Isomerase Prevent Cleavage of Disulfide Bonds in Receptor-bound Glycoprotein 120 and Prevent HIV-1 Entry. J. Biol. Chem. 277: 50579-50588 [Abstract] [Full Text]  
  • Reeves, J. D., Doms, R. W. (2002). Human immunodeficiency virus type 2. J. Gen. Virol. 83: 1253-1265 [Full Text]  
  • Puffer, B. A., Pohlmann, S., Edinger, A. L., Carlin, D., Sanchez, M. D., Reitter, J., Watry, D. D., Fox, H. S., Desrosiers, R. C., Doms, R. W. (2002). CD4 Independence of Simian Immunodeficiency Virus Envs Is Associated with Macrophage Tropism, Neutralization Sensitivity, and Attenuated Pathogenicity. J. Virol. 76: 2595-2605 [Abstract] [Full Text]  
  • Edwards, T. G., Wyss, S., Reeves, J. D., Zolla-Pazner, S., Hoxie, J. A., Doms, R. W., Baribaud, F. (2002). Truncation of the Cytoplasmic Domain Induces Exposure of Conserved Regions in the Ectodomain of Human Immunodeficiency Virus Type 1 Envelope Protein. J. Virol. 76: 2683-2691 [Abstract] [Full Text]  
  • Foley, H. D., Otero, M., Orenstein, J. M., Pomerantz, R. J., Schnell, M. J. (2002). Rhabdovirus-Based Vectors with Human Immunodeficiency Virus Type 1 (HIV-1) Envelopes Display HIV-1-Like Tropism and Target Human Dendritic Cells. J. Virol. 76: 19-31 [Abstract] [Full Text]  
  • Lin, G., Lee, B., Haggarty, B. S., Doms, R. W., Hoxie, J. A. (2001). CD4-Independent Use of Rhesus CCR5 by Human Immunodeficiency Virus Type 2 Implicates an Electrostatic Interaction between the CCR5 N Terminus and the gp120 C4 Domain. J. Virol. 75: 10766-10778 [Abstract] [Full Text]  
  • Overbaugh, J., Miller, A. D., Eiden, M. V. (2001). Receptors and Entry Cofactors for Retroviruses Include Single and Multiple Transmembrane-Spanning Proteins as well as Newly Described Glycophosphatidylinositol-Anchored and Secreted Proteins. Microbiol. Mol. Biol. Rev. 65: 371-389 [Abstract] [Full Text]  
  • Lane, B. R., Strieter, R. M., Coffey, M. J., Markovitz, D. M. (2001). Human Immunodeficiency Virus Type 1 (HIV-1)-Induced GRO-{alpha} Production Stimulates HIV-1 Replication in Macrophages and T Lymphocytes. J. Virol. 75: 5812-5822 [Abstract] [Full Text]  
  • Lehner, T., Doyle, C., Wang, Y., Babaahmady, K., Whittall, T., Tao, L., Bergmeier, L., Kelly, C. (2001). Immunogenicity of the Extracellular Domains of C-C Chemokine Receptor 5 and the In Vitro Effects on Simian Immunodeficiency Virus or HIV Infectivity. J. Immunol. 166: 7446-7455 [Abstract] [Full Text]  
  • Edwards, T. G., Hoffman, T. L., Baribaud, F., Wyss, S., LaBranche, C. C., Romano, J., Adkinson, J., Sharron, M., Hoxie, J. A., Doms, R. W. (2001). Relationships between CD4 Independence, Neutralization Sensitivity, and Exposure of a CD4-Induced Epitope in a Human Immunodeficiency Virus Type 1 Envelope Protein. J. Virol. 75: 5230-5239 [Abstract] [Full Text]  
  • Dumonceaux, J., Goujon, C., Joliot, V., Briand, P., Hazan, U. (2001). Determination of Essential Amino Acids Involved in the CD4-Independent Tropism of the X4 Human Immunodeficiency Virus Type 1 m7NDK Isolate: Role of Potential N Glycosylations in the C2 and V3 Regions of gp120. J. Virol. 75: 5425-5428 [Abstract] [Full Text]  
  • de Parseval, A., Elder, J. H. (2001). Binding of Recombinant Feline Immunodeficiency Virus Surface Glycoprotein to Feline Cells: Role of CXCR4, Cell-Surface Heparans, and an Unidentified Non-CXCR4 Receptor. J. Virol. 75: 4528-4539 [Abstract] [Full Text]  
  • Martín, J., LaBranche, C. C., González-Scarano, F. (2001). Differential CD4/CCR5 Utilization, gp120 Conformation, and Neutralization Sensitivity between Envelopes from a Microglia-Adapted Human Immunodeficiency Virus Type 1 and Its Parental Isolate. J. Virol. 75: 3568-3580 [Abstract] [Full Text]  
  • Kolchinsky, P., Kiprilov, E., Bartley, P., Rubinstein, R., Sodroski, J. (2001). Loss of a Single N-Linked Glycan Allows CD4-Independent Human Immunodeficiency Virus Type 1 Infection by Altering the Position of the gp120 V1/V2 Variable Loops. J. Virol. 75: 3435-3443 [Abstract] [Full Text]  
  • Kolchinsky, P., Kiprilov, E., Sodroski, J. (2001). Increased Neutralization Sensitivity of CD4-Independent Human Immunodeficiency Virus Variants. J. Virol. 75: 2041-2050 [Abstract] [Full Text]  
  • Holmen, S. L., Melder, D. C., Federspiel, M. J. (2001). Identification of Key Residues in Subgroup A Avian Leukosis Virus Envelope Determining Receptor Binding Affinity and Infectivity of Cells Expressing Chicken or Quail Tva Receptor. J. Virol. 75: 726-737 [Abstract] [Full Text]  
  • Valente, S. T., Chanel, C., Dumonceaux, J., Olivier, R., Marullo, S., Briand, P., Hazan, U. (2001). CXCR4 Is Down-Regulated in Cells Infected with the CD4-Independent X4 Human Immunodeficiency Virus Type 1 Isolate m7NDK. J. Virol. 75: 439-447 [Abstract] [Full Text]  
  • Zhang, Y.-j., Lou, B., Lal, R. B., Gettie, A., Marx, P. A., Moore, J. P. (2000). Use of Inhibitors To Evaluate Coreceptor Usage by Simian and Simian/Human Immunodeficiency Viruses and Human Immunodeficiency Virus Type 2 in Primary Cells. J. Virol. 74: 6893-6910 [Abstract] [Full Text]  
  • Borsetti, A., Parolin, C., Ridolfi, B., Sernicola, L., Geraci, A., Ensoli, B., Titti, F. (2000). CD4-Independent Infection of Two CD4-/CCR5-/CXCR4+ Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses. J. Virol. 74: 6689-6694 [Abstract] [Full Text]  
  • Lerner, D. L., Elder, J. H. (2000). Expanded Host Cell Tropism and Cytopathic Properties of Feline Immunodeficiency Virus Strain PPR Subsequent to Passage through Interleukin-2-Independent T Cells. J. Virol. 74: 1854-1863 [Abstract] [Full Text]  
  • Shieh, J. T. C., Martín, J., Baltuch, G., Malim, M. H., González-Scarano, F. (2000). Determinants of Syncytium Formation in Microglia by Human Immunodeficiency Virus Type 1: Role of the V1/V2 Domains. J. Virol. 74: 693-701 [Abstract] [Full Text]  
  • LaBranche, C. C., Hoffman, T. L., Romano, J., Haggarty, B. S., Edwards, T. G., Matthews, T. J., Doms, R. W., Hoxie, J. A. (1999). Determinants of CD4 Independence for a Human Immunodeficiency Virus Type 1 Variant Map outside Regions Required for Coreceptor Specificity. J. Virol. 73: 10310-10319 [Abstract] [Full Text]  
  • Kolchinsky, P., Mirzabekov, T., Farzan, M., Kiprilov, E., Cayabyab, M., Mooney, L. J., Choe, H., Sodroski, J. (1999). Adaptation of a CCR5-Using, Primary Human Immunodeficiency Virus Type 1 Isolate for CD4-Independent Replication. J. Virol. 73: 8120-8126 [Abstract] [Full Text]  
  • Mirzabekov, T., Bannert, N., Farzan, M., Hofmann, W., Kolchinsky, P., Wu, L., Wyatt, R., Sodroski, J. (1999). Enhanced Expression, Native Purification, and Characterization of CCR5, a Principal HIV-1 Coreceptor. J. Biol. Chem. 274: 28745-28750 [Abstract] [Full Text]  
  • Nisole, S., Krust, B., Callebaut, C., Guichard, G., Muller, S., Briand, J.-P., Hovanessian, A. G. (1999). The Anti-HIV Pseudopeptide HB-19 Forms a Complex with the Cell-surface-expressed Nucleolin Independent of Heparan Sulfate Proteoglycans. J. Biol. Chem. 274: 27875-27884 [Abstract] [Full Text]  
  • Murakami, T., Zhang, T.-Y., Koyanagi, Y., Tanaka, Y., Kim, J., Suzuki, Y., Minoguchi, S., Tamamura, H., Waki, M., Matsumoto, A., Fujii, N., Shida, H., Hoxie, J. A., Peiper, S. C., Yamamoto, N. (1999). Inhibitory Mechanism of the CXCR4 Antagonist T22 against Human Immunodeficiency Virus Type 1 Infection. J. Virol. 73: 7489-7496 [Abstract] [Full Text]  
  • Dumonceaux, J., Chanel, C., Valente, S., Quivet, L., Briand, P., Hazan, U. (1999). Mutations in the env gene of human immunodeficiency virus type 1 NDK isolates and the use of African green monkey CXCR4 as a co-receptor in COS-7 cells. J. Gen. Virol. 80: 1975-1982 [Abstract] [Full Text]  
  • Schenten, D., Marcon, L., Karlsson, G. B., Parolin, C., Kodama, T., Gerard, N., Sodroski, J. (1999). Effects of Soluble CD4 on Simian Immunodeficiency Virus Infection of CD4-Positive and CD4-Negative Cells. J. Virol. 73: 5373-5380 [Abstract] [Full Text]  
  • Hoffman, T. L., LaBranche, C. C., Zhang, W., Canziani, G., Robinson, J., Chaiken, I., Hoxie, J. A., Doms, R. W. (1999). Stable exposure of the coreceptor-binding site in a CD4-independent HIV-1 envelope protein. Proc. Natl. Acad. Sci. USA 96: 6359-6364 [Abstract] [Full Text]  
  • Maréchal, V., Arenzana-Seisdedos, F., Heard, J.-M., Schwartz, O. (1999). Opposite Effects of SDF-1 on Human Immunodeficiency Virus Type 1 Replication. J. Virol. 73: 3608-3615 [Abstract] [Full Text]  
  • Richardson, J., Pancino, G., Merat, R., Leste-Lasserre, T., Moraillon, A., Schneider-Mergener, J., Alizon, M., Sonigo, P., Heveker, N. (1999). Shared Usage of the Chemokine Receptor CXCR4 by Primary and Laboratory-Adapted Strains of Feline Immunodeficiency Virus. J. Virol. 73: 3661-3671 [Abstract] [Full Text]  
  • Lee, B., Ratajczak, J., Doms, R. W., Gewirtz, A. M., Ratajczak, M. Z. (1999). Coreceptor/Chemokine Receptor Expression on Human Hematopoietic Cells: Biological Implications for Human Immunodeficiency Virus-Type 1 Infection. Blood 93: 1145-1156 [Abstract] [Full Text]  
  • Ohagen, A., Ghosh, S., He, J., Huang, K., Chen, Y., Yuan, M., Osathanondh, R., Gartner, S., Shi, B., Shaw, G., Gabuzda, D. (1999). Apoptosis Induced by Infection of Primary Brain Cultures with Diverse Human Immunodeficiency Virus Type 1 Isolates: Evidence for a Role of the Envelope. J. Virol. 73: 897-906 [Abstract] [Full Text]  
  • Follis, K. E., Trahey, M., LaCasse, R. A., Nunberg, J. H. (1998). Continued Utilization of CCR5 Coreceptor by a Newly Derived T-Cell Line-Adapted Isolate of Human Immunodeficiency Virus Type 1. J. Virol. 72: 7603-7608 [Abstract] [Full Text]  
  • Wyatt, R., Sodroski, J. (1998). The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens. Science 280: 1884-1888 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»