This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holzer, G. W.
Right arrow Articles by Falkner, F. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holzer, G. W.
Right arrow Articles by Falkner, F. G.

 Previous Article  |  Next Article 

J. Virol., 07 1997, 4997-5002, Vol 71, No. 7
Copyright © 1997, American Society for Microbiology

Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line

GW Holzer and FG Falkner
IMMUNO AG, Biomedical Research Center, Orth/Donau, Austria.

The vaccinia virus D4R open reading frame, encoding the essential DNA repair enzyme uracil DNA glycosylase, was expressed in two permanent cell lines, the rabbit kidney cell line RK13 and the human fibroblast cell line 293. The temperature-sensitive vaccinia virus mutant ts4149, which maps within D4R, was able to grow under restrictive conditions in both of these transformed cell lines. Cell clones complemented D4R function to various degrees, demonstrating complementation of an essential vaccinia virus gene by a cell line constitutively expressing the essential function. Thus, the complementing host cells allowed the rescue of a virus defective in the D4R gene, demonstrating that this system may be used for the propagation of defective cytoplasmic DNA viruses. The defective virus grew to high yields only in the engineered cell lines. The data support the hypothesis that early gene products, such as uracil DNA glycosylase, supplied in trans can fully complement essential viral functions.


This article has been cited by other articles:

  • Mayrhofer, J., Coulibaly, S., Hessel, A., Holzer, G. W., Schwendinger, M., Bruhl, P., Gerencer, M., Crowe, B. A., Shuo, S., Hong, W., Tan, Y. J., Dietrich, B., Sabarth, N., Savidis-Dacho, H., Kistner, O., Barrett, P. N., Falkner, F. G. (2009). Nonreplicating Vaccinia Virus Vectors Expressing the H5 Influenza Virus Hemagglutinin Produced in Modified Vero Cells Induce Robust Protection. J. Virol. 83: 5192-5203 [Abstract] [Full Text]  
  • da Fonseca, F. G., Weisberg, A. S., Caeiro, M. F., Moss, B. (2004). Vaccinia Virus Mutants with Alanine Substitutions in the Conserved G5R Gene Fail To Initiate Morphogenesis at the Nonpermissive Temperature. J. Virol. 78: 10238-10248 [Abstract] [Full Text]  
  • Scaramozzino, N., Sanz, G., Crance, J. M., Saparbaev, M., Drillien, R., Laval, J., Kavli, B., Garin, D. (2003). Characterisation of the substrate specificity of homogeneous vaccinia virus uracil-DNA glycosylase. Nucleic Acids Res 31: 4950-4957 [Abstract] [Full Text]  
  • Konetschny, C., Holzer, G. W., Urban, C., Hammerle, T., Mayrhofer, J., Falkner, F. G. (2003). Generation of Transduction-Competent Retroviral Vectors by Infection with a Single Hybrid Vaccinia Virus. J. Virol. 77: 7017-7025 [Abstract] [Full Text]  
  • De Silva, F. S., Moss, B. (2002). Vaccinia Virus Uracil DNA Glycosylase Has an Essential Role in DNA Synthesis That Is Independent of Its Glycosylase Activity: Catalytic Site Mutations Reduce Virulence but Not Virus Replication in Cultured Cells. J. Virol. 77: 159-166 [Abstract] [Full Text]  
  • Ober, B. T., Bruhl, P., Schmidt, M., Wieser, V., Gritschenberger, W., Coulibaly, S., Savidis-Dacho, H., Gerencer, M., Falkner, F. G. (2002). Immunogenicity and Safety of Defective Vaccinia Virus Lister: Comparison with Modified Vaccinia Virus Ankara. J. Virol. 76: 7713-7723 [Abstract] [Full Text]  
  • Ishii, K., Moss, B. (2001). Role of Vaccinia Virus A20R Protein in DNA Replication: Construction and Characterization of Temperature-Sensitive Mutants. J. Virol. 75: 1656-1663 [Abstract] [Full Text]  
  • Holzer, G. W., Remp, G., Antoine, G., Pfleiderer, M., Enzersberger, O. M., Emsenhuber, W., Hämmerle, T., Gruber, F., Urban, C., Falkner, F. G., Dorner, F. (1999). Highly Efficient Induction of Protective Immunity by a Vaccinia Virus Vector Defective in Late Gene Expression. J. Virol. 73: 4536-4542 [Abstract] [Full Text]  
  • Borrego, B, Lorenzo, M., Blasco, R (1999). Complementation of P37 (F13L gene) knock-out in vaccinia virus by a cell line expressing the gene constitutively. J. Gen. Virol. 80: 425-432 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»