This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Q.
Right arrow Articles by Hutt-Fletcher, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Q.
Right arrow Articles by Hutt-Fletcher, L. M.

 Previous Article  |  Next Article 

J. Virol., Jun 1997, 4657-4662, Vol 71, No. 6
Copyright © 1997, American Society for Microbiology

Epstein-Barr virus uses HLA class II as a cofactor for infection of B lymphocytes

Q Li, MK Spriggs, S Kovats, SM Turk, MR Comeau, B Nepom and LM Hutt-Fletcher
School of Biological Sciences, University of Missouri-Kansas City, 64110, USA.

Infection of B lymphocytes by Epstein-Barr virus (EBV) requires attachment of virus via binding of viral glycoprotein gp350 to CD21 on the cell surface. Penetration of the cell membrane additionally involves a complex of three glycoproteins, gH, gL, and gp42. Glycoprotein gp42 binds to HLA-DR. Interference with this interaction with a soluble form of gp42, with a monoclonal antibody (MAb) to gp42, or with a MAb to HLA-DR inhibited virus infection. It was not possible to superinfect cells that failed to express HLA-DR unless expression was restored by transfection or creation of hybrid cell lines with complementing deficiencies in expression of HLA class II. HLA class II molecules thus serve as cofactors for infection of human B cells.


This article has been cited by other articles:

  • Plate, A. E., Smajlovic, J., Jardetzky, T. S., Longnecker, R. (2009). Functional Analysis of Glycoprotein L (gL) from Rhesus Lymphocryptovirus in Epstein-Barr Virus-Mediated Cell Fusion Indicates a Direct Role of gL in gB-Induced Membrane Fusion. J. Virol. 83: 7678-7689 [Abstract] [Full Text]  
  • Sorem, J., Jardetzky, T. S., Longnecker, R. (2009). Cleavage and Secretion of Epstein-Barr Virus Glycoprotein 42 Promote Membrane Fusion with B Lymphocytes. J. Virol. 83: 6664-6672 [Abstract] [Full Text]  
  • Backovic, M., Longnecker, R., Jardetzky, T. S. (2009). Structure of a trimeric variant of the Epstein-Barr virus glycoprotein B. Proc. Natl. Acad. Sci. USA 106: 2880-2885 [Abstract] [Full Text]  
  • Reimer, J. J., Backovic, M., Deshpande, C. G., Jardetzky, T., Longnecker, R. (2009). Analysis of Epstein-Barr Virus Glycoprotein B Functional Domains via Linker Insertion Mutagenesis. J. Virol. 83: 734-747 [Abstract] [Full Text]  
  • Hahn, A., Birkmann, A., Wies, E., Dorer, D., Mahr, K., Sturzl, M., Titgemeyer, F., Neipel, F. (2009). Kaposi's Sarcoma-Associated Herpesvirus gH/gL: Glycoprotein Export and Interaction with Cellular Receptors. J. Virol. 83: 396-407 [Abstract] [Full Text]  
  • Veettil, M. V., Sadagopan, S., Sharma-Walia, N., Wang, F.-Z., Raghu, H., Varga, L., Chandran, B. (2008). Kaposi's Sarcoma-Associated Herpesvirus Forms a Multimolecular Complex of Integrins ({alpha}V{beta}5, {alpha}V{beta}3, and {alpha}3{beta}1) and CD98-xCT during Infection of Human Dermal Microvascular Endothelial Cells, and CD98-xCT Is Essential for the Postentry Stage of Infection. J. Virol. 82: 12126-12144 [Abstract] [Full Text]  
  • Dorner, M., Zucol, F., Berger, C., Byland, R., Melroe, G. T., Bernasconi, M., Speck, R. F., Nadal, D. (2008). Distinct Ex Vivo Susceptibility of B-Cell Subsets to Epstein-Barr Virus Infection According to Differentiation Status and Tissue Origin. J. Virol. 82: 4400-4412 [Abstract] [Full Text]  
  • De Jager, P. L., Simon, K. C., Munger, K. L., Rioux, J. D., Hafler, D. A., Ascherio, A. (2008). Integrating risk factors: HLA-DRB1*1501 and Epstein-Barr virus in multiple sclerosis. Neurology 70: 1113-1118 [Abstract] [Full Text]  
  • Wang, D., Yu, Q.-C., Schroer, J., Murphy, E., Shenk, T. (2007). Human cytomegalovirus uses two distinct pathways to enter retinal pigmented epithelial cells. Proc. Natl. Acad. Sci. USA 104: 20037-20042 [Abstract] [Full Text]  
  • Kirschner, A. N., Lowrey, A. S., Longnecker, R., Jardetzky, T. S. (2007). Binding-Site Interactions between Epstein-Barr Virus Fusion Proteins gp42 and gH/gL Reveal a Peptide That Inhibits both Epithelial and B-Cell Membrane Fusion. J. Virol. 81: 9216-9229 [Abstract] [Full Text]  
  • Wu, L., Hutt-Fletcher, L. M. (2007). Compatibility of the gH homologues of Epstein-Barr virus and related lymphocryptoviruses. J. Gen. Virol. 88: 2129-2136 [Abstract] [Full Text]  
  • Simon, K., Yang, X., Munger, K., Ascherio, A. (2007). Variation in the Epstein-Barr virus receptor, CR2, and risk of multiple sclerosis. Mult Scler 13: 947-948  
  • Hutt-Fletcher, L. M. (2007). Epstein-Barr Virus Entry. J. Virol. 81: 7825-7832 [Full Text]  
  • Lunemann, J. D., Kamradt, T., Martin, R., Munz, C. (2007). Epstein-Barr Virus: Environmental Trigger of Multiple Sclerosis?. J. Virol. 81: 6777-6784 [Full Text]  
  • Wiertz, E. J., Devlin, R., Collins, H. L., Ressing, M. E. (2007). Herpesvirus Interference with Major Histocompatibility Complex Class II-Restricted T-Cell Activation. J. Virol. 81: 4389-4396 [Full Text]  
  • Kirschner, A. N., Omerovic, J., Popov, B., Longnecker, R., Jardetzky, T. S. (2006). Soluble Epstein-Barr Virus Glycoproteins gH, gL, and gp42 Form a 1:1:1 Stable Complex That Acts Like Soluble gp42 in B-Cell Fusion but Not in Epithelial Cell Fusion. J. Virol. 80: 9444-9454 [Abstract] [Full Text]  
  • Turk, S. M., Jiang, R., Chesnokova, L. S., Hutt-Fletcher, L. M. (2006). Antibodies to gp350/220 Enhance the Ability of Epstein-Barr Virus To Infect Epithelial Cells. J. Virol. 80: 9628-9633 [Abstract] [Full Text]  
  • Jiang, R., Scott, R. S., Hutt-Fletcher, L. M. (2006). Epstein-Barr Virus Shed in Saliva Is High in B-Cell-Tropic Glycoprotein gp42. J. Virol. 80: 7281-7283 [Abstract] [Full Text]  
  • Shannon-Lowe, C. D., Neuhierl, B., Baldwin, G., Rickinson, A. B., Delecluse, H.-J. (2006). From the Cover: Resting B cells as a transfer vehicle for Epstein-Barr virus infection of epithelial cells. Proc. Natl. Acad. Sci. USA 103: 7065-7070 [Abstract] [Full Text]  
  • Wang, D., Shenk, T. (2005). Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism. Proc. Natl. Acad. Sci. USA 102: 18153-18158 [Abstract] [Full Text]  
  • Shannon-Lowe, C., Baldwin, G., Feederle, R., Bell, A., Rickinson, A., Delecluse, H.-J. (2005). Epstein-Barr virus-induced B-cell transformation: quantitating events from virus binding to cell outgrowth. J. Gen. Virol. 86: 3009-3019 [Abstract] [Full Text]  
  • Urquiza, M., Lopez, R., Patino, H., Rosas, J. E., Patarroyo, M. E. (2005). Identification of Three gp350/220 Regions Involved in Epstein-Barr Virus Invasion of Host Cells. J. Biol. Chem. 280: 35598-35605 [Abstract] [Full Text]  
  • Omerovic, J., Lev, L., Longnecker, R. (2005). The Amino Terminus of Epstein-Barr Virus Glycoprotein gH Is Important for Fusion with Epithelial and B Cells. J. Virol. 79: 12408-12415 [Abstract] [Full Text]  
  • Wu, L., Borza, C. M., Hutt-Fletcher, L. M. (2005). Mutations of Epstein-Barr Virus gH That Are Differentially Able To Support Fusion with B Cells or Epithelial Cells. J. Virol. 79: 10923-10930 [Abstract] [Full Text]  
  • Ressing, M. E., van Leeuwen, D., Verreck, F. A. W., Keating, S., Gomez, R., Franken, K. L. M. C., Ottenhoff, T. H. M., Spriggs, M., Schumacher, T. N., Hutt-Fletcher, L. M., Rowe, M., Wiertz, E. J. H. J. (2005). Epstein-Barr Virus gp42 Is Posttranslationally Modified To Produce Soluble gp42 That Mediates HLA Class II Immune Evasion. J. Virol. 79: 841-852 [Abstract] [Full Text]  
  • Parry, C., Bell, S., Minson, T., Browne, H. (2005). Herpes simplex virus type 1 glycoprotein H binds to {alpha}v{beta}3 integrins. J. Gen. Virol. 86: 7-10 [Abstract] [Full Text]  
  • Johannsen, E., Luftig, M., Chase, M. R., Weicksel, S., Cahir-McFarland, E., Illanes, D., Sarracino, D., Kieff, E. (2004). Proteins of purified Epstein-Barr virus. Proc. Natl. Acad. Sci. USA 101: 16286-16291 [Abstract] [Full Text]  
  • Guerreiro-Cacais, A. O., Li, L., Donati, D., Bejarano, M. T., Morgan, A., Masucci, M. G., Hutt-Fletcher, L., Levitsky, V. (2004). Capacity of Epstein-Barr virus to infect monocytes and inhibit their development into dendritic cells is affected by the cell type supporting virus replication. J. Gen. Virol. 85: 2767-2778 [Abstract] [Full Text]  
  • Stewart, J. P., Silvia, O. J., Atkin, I. M. D., Hughes, D. J., Ebrahimi, B., Adler, H. (2004). In Vivo Function of a Gammaherpesvirus Virion Glycoprotein: Influence on B-Cell Infection and Mononucleosis. J. Virol. 78: 10449-10459 [Abstract] [Full Text]  
  • Silva, A. L., Omerovic, J., Jardetzky, T. S., Longnecker, R. (2004). Mutational Analyses of Epstein-Barr Virus Glycoprotein 42 Reveal Functional Domains Not Involved in Receptor Binding but Required for Membrane Fusion. J. Virol. 78: 5946-5956 [Abstract] [Full Text]  
  • Gill, M. B., Roecklein-Canfield, J., Sage, D. R., Zambela-Soediono, M., Longtine, N., Uknis, M., Fingeroth, J. D. (2004). EBV attachment stimulates FHOS/FHOD1 redistribution and co-aggregation with CD21: formin interactions with the cytoplasmic domain of human CD21. J. Cell Sci. 117: 2709-2720 [Abstract] [Full Text]  
  • Borza, C. M., Morgan, A. J., Turk, S. M., Hutt-Fletcher, L. M. (2004). Use of gHgL for Attachment of Epstein-Barr Virus to Epithelial Cells Compromises Infection. J. Virol. 78: 5007-5014 [Abstract] [Full Text]  
  • de Lima, B. D., May, J. S., Stevenson, P. G. (2004). Murine Gammaherpesvirus 68 Lacking gp150 Shows Defective Virion Release but Establishes Normal Latency In Vivo. J. Virol. 78: 5103-5112 [Abstract] [Full Text]  
  • Isobe, Y., Sugimoto, K., Yang, L., Tamayose, K., Egashira, M., Kaneko, T., Takada, K., Oshimi, K. (2004). Epstein-Barr Virus Infection of Human Natural Killer Cell Lines and Peripheral Blood Natural Killer Cells. Cancer Res. 64: 2167-2174 [Abstract] [Full Text]  
  • Spear, P. G., Longnecker, R. (2003). Herpesvirus Entry: an Update. J. Virol. 77: 10179-10185 [Full Text]  
  • Ressing, M. E., van Leeuwen, D., Verreck, F. A. W., Gomez, R., Heemskerk, B., Toebes, M., Mullen, M. M., Jardetzky, T. S., Longnecker, R., Schilham, M. W., Ottenhoff, T. H. M., Neefjes, J., Schumacher, T. N., Hutt-Fletcher, L. M., Wiertz, E. J. H. J. (2003). Interference with T cell receptor-HLA-DR interactions by Epstein-Barr virus gp42 results in reduced T helper cell recognition. Proc. Natl. Acad. Sci. USA 100: 11583-11588 [Abstract] [Full Text]  
  • Andreone, P., Gramenzi, A., Lorenzini, S., Biselli, M., Cursaro, C., Pileri, S., Bernardi, M. (2003). Posttransplantation Lymphoproliferative Disorders. Arch Intern Med 163: 1997-2004 [Abstract] [Full Text]  
  • Santoro, F., Greenstone, H. L., Insinga, A., Liszewski, M. K., Atkinson, J. P., Lusso, P., Berger, E. A. (2003). Interaction of Glycoprotein H of Human Herpesvirus 6 with the Cellular Receptor CD46. J. Biol. Chem. 278: 25964-25969 [Abstract] [Full Text]  
  • McShane, M. P., Mullen, M. M., Haan, K. M., Jardetzky, T. S., Longnecker, R. (2003). Mutational Analysis of the HLA Class II Interaction with Epstein-Barr Virus Glycoprotein 42. J. Virol. 77: 7655-7662 [Abstract] [Full Text]  
  • Tabiasco, J., Vercellone, A., Meggetto, F., Hudrisier, D., Brousset, P., Fournie, J.-J. (2003). Acquisition of Viral Receptor by NK Cells Through Immunological Synapse. J. Immunol. 170: 5993-5998 [Abstract] [Full Text]  
  • Mori, Y., Yang, X., Akkapaiboon, P., Okuno, T., Yamanishi, K. (2003). Human Herpesvirus 6 Variant A Glycoprotein H-Glycoprotein L-Glycoprotein Q Complex Associates with Human CD46. J. Virol. 77: 4992-4999 [Abstract] [Full Text]  
  • Kudoh, A., Fujita, M., Kiyono, T., Kuzushima, K., Sugaya, Y., Izuta, S., Nishiyama, Y., Tsurumi, T. (2002). Reactivation of Lytic Replication from B Cells Latently Infected with Epstein-Barr Virus Occurs with High S-Phase Cyclin-Dependent Kinase Activity while Inhibiting Cellular DNA Replication. J. Virol. 77: 851-861 [Abstract] [Full Text]  
  • Neuhierl, B., Feederle, R., Hammerschmidt, W., Delecluse, H. J. (2002). Glycoprotein gp110 of Epstein-Barr virus determines viral tropism and efficiency of infection. Proc. Natl. Acad. Sci. USA 99: 15036-15041 [Abstract] [Full Text]  
  • Rivailler, P., Cho, Y.-g., Wang, F. (2002). Complete Genomic Sequence of an Epstein-Barr Virus-Related Herpesvirus Naturally Infecting a New World Primate: a Defining Point in the Evolution of Oncogenic Lymphocryptoviruses. J. Virol. 76: 12055-12068 [Abstract] [Full Text]  
  • Harman, A., Browne, H., Minson, T. (2002). The Transmembrane Domain and Cytoplasmic Tail of Herpes Simplex Virus Type 1 Glycoprotein H Play a Role in Membrane Fusion. J. Virol. 76: 10708-10716 [Abstract] [Full Text]  
  • Dezube, B. J., Zambela, M., Sage, D. R., Wang, J.-F., Fingeroth, J. D. (2002). Characterization of Kaposi sarcoma-associated herpesvirus/human herpesvirus-8 infection of human vascular endothelial cells: early events. Blood 100: 888-896 [Abstract] [Full Text]  
  • Li, L., Liu, D., Hutt-Fletcher, L., Morgan, A., Masucci, M. G., Levitsky, V. (2002). Epstein-Barr virus inhibits the development of dendritic cells by promoting apoptosis of their monocyte precursors in the presence of granulocyte macrophage-colony-stimulating factor and interleukin-4. Blood 99: 3725-3734 [Abstract] [Full Text]  
  • Ascherio, A., Munger, K. L., Lennette, E. T., Spiegelman, D., Hernan, M. A., Olek, M. J., Hankinson, S. E., Hunter, D. J. (2001). Epstein-Barr Virus Antibodies and Risk of Multiple Sclerosis: A Prospective Study. JAMA 286: 3083-3088 [Abstract] [Full Text]  
  • Maruo, S., Yang, L., Takada, K. (2001). Roles of Epstein-Barr virus glycoproteins gp350 and gp25 in the infection of human epithelial cells. J. Gen. Virol. 82: 2373-2383 [Abstract] [Full Text]  
  • Haan, K. M., Aiyar, A., Longnecker, R. (2001). Establishment of Latent Epstein-Barr Virus Infection and Stable Episomal Maintenance in Murine B-Cell Lines. J. Virol. 75: 3016-3020 [Abstract] [Full Text]  
  • Lake, C. M., Hutt-Fletcher, L. M. (2000). Epstein-Barr Virus That Lacks Glycoprotein gN Is Impaired in Assembly and Infection. J. Virol. 74: 11162-11172 [Abstract] [Full Text]  
  • Janz, A., Oezel, M., Kurzeder, C., Mautner, J., Pich, D., Kost, M., Hammerschmidt, W., Delecluse, H.-J. (2000). Infectious Epstein-Barr Virus Lacking Major Glycoprotein BLLF1 (gp350/220) Demonstrates the Existence of Additional Viral Ligands. J. Virol. 74: 10142-10152 [Abstract] [Full Text]  
  • Joseph, A. M., Babcock, G. J., Thorley-Lawson, D. A. (2000). EBV Persistence Involves Strict Selection of Latently Infected B Cells. J. Immunol. 165: 2975-2981 [Abstract] [Full Text]  
  • Marchbank, K. J., Watson, C. C., Ritsema, D. F., Holers, V. M. (2000). Expression of Human Complement Receptor 2 (CR2, CD21) in Cr2-/- Mice Restores Humoral Immune Function. J. Immunol. 165: 2354-2361 [Abstract] [Full Text]  
  • Cohen, J. I. (2000). Epstein-Barr Virus Infection. NEJM 343: 481-492 [Full Text]  
  • Haan, K. M., Longnecker, R. (2000). Coreceptor restriction within the HLA-DQ locus for Epstein-Barr virus infection. Proc. Natl. Acad. Sci. USA 10.1073/pnas.160171697v1 [Abstract] [Full Text]  
  • Molesworth, S. J., Lake, C. M., Borza, C. M., Turk, S. M., Hutt-Fletcher, L. M. (2000). Epstein-Barr Virus gH Is Essential for Penetration of B Cells but Also Plays a Role in Attachment of Virus to Epithelial Cells. J. Virol. 74: 6324-6332 [Abstract] [Full Text]  
  • Haan, K. M., Kwok, W. W., Longnecker, R., Speck, P. (2000). Epstein-Barr Virus Entry Utilizing HLA-DP or HLA-DQ as a Coreceptor. J. Virol. 74: 2451-2454 [Abstract] [Full Text]  
  • Fingeroth, J. D., Diamond, M. E., Sage, D. R., Hayman, J., Yates, J. L. (1999). CD21-Dependent Infection of an Epithelial Cell Line, 293, by Epstein-Barr Virus. J. Virol. 73: 2115-2125 [Abstract] [Full Text]  
  • Stevenson, P. G., Doherty, P. C. (1999). Non-Antigen-Specific B-Cell Activation following Murine Gammaherpesvirus Infection Is CD4 Independent In Vitro but CD4 Dependent In Vivo. J. Virol. 73: 1075-1079 [Abstract] [Full Text]  
  • Borza, C. M., Hutt-Fletcher, L. M. (1998). Epstein-Barr Virus Recombinant Lacking Expression of Glycoprotein gp150 Infects B Cells Normally but Is Enhanced for Infection of Epithelial Cells. J. Virol. 72: 7577-7582 [Abstract] [Full Text]  
  • Wang, X., Kenyon, W. J., Li, Q., Mullberg, J., Hutt-Fletcher, L. M. (1998). Epstein-Barr Virus Uses Different Complexes of Glycoproteins gH and gL To Infect B Lymphocytes and Epithelial Cells. J. Virol. 72: 5552-5558 [Abstract] [Full Text]  
  • Lake, C. M., Molesworth, S. J., Hutt-Fletcher, L. M. (1998). The Epstein-Barr Virus (EBV) gN Homolog BLRF1 Encodes a 15-Kilodalton Glycoprotein That Cannot Be Authentically Processed unless It Is Coexpressed with the EBV gM Homolog BBRF3. J. Virol. 72: 5559-5564 [Abstract] [Full Text]  
  • Imai, S., Nishikawa, J., Takada, K. (1998). Cell-to-Cell Contact as an Efficient Mode of Epstein-Barr Virus Infection of Diverse Human Epithelial Cells. J. Virol. 72: 4371-4378 [Abstract] [Full Text]  
  • Grigg, M. E., McMahon, C. W., Morkowski, S., Rudensky, A. Y., Pullen, A. M. (1998). Mtv-1 Superantigen Trafficks Independently of Major Histocompatibility Complex Class II Directly to the B-Cell Surface by the Exocytic Pathway. J. Virol. 72: 2577-2588 [Abstract] [Full Text]  
  • Wang, X., Hutt-Fletcher, L. M. (1998). Epstein-Barr Virus Lacking Glycoprotein gp42 Can Bind to B Cells but Is Not Able To Infect. J. Virol. 72: 158-163 [Abstract] [Full Text]  
  • Haan, K. M., Longnecker, R. (2000). Coreceptor restriction within the HLA-DQ locus for Epstein-Barr virus infection. Proc. Natl. Acad. Sci. USA 97: 9252-9257 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»