This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Swingler, S.
Right arrow Articles by Trono, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Swingler, S.
Right arrow Articles by Trono, D.

 Previous Article  |  Next Article 

J. Virol., 06 1997, 4372-4377, Vol 71, No. 6
Copyright © 1997, American Society for Microbiology

The Nef protein of human immunodeficiency virus type 1 enhances serine phosphorylation of the viral matrix

S Swingler, P Gallay, D Camaur, J Song, A Abo and D Trono
Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.

The human immunodeficiency virus type 1 matrix (MA) protein is phosphorylated during virion maturation on its C-terminal tyrosine and on several serine residues. Whereas MA tyrosine phosphorylation facilitates viral nuclear import, the significance of MA serine phosphorylation remains unclear. Here, we report that MA serine but not tyrosine phosphorylation is strongly enhanced by Nef. Mutations that abrogated the membrane association of Nef and its ability to bind a cellular serine/threonine kinase greatly diminished the extent of virion MA serine phosphorylation. Correspondingly, a protein kinase coimmunoprecipitated with Nef could phosphorylate MA on serine in vitro, producing a phosphopeptide pattern reminiscent of that of virion MA. Recombinant p21-activated kinase hPAK65, a recently proposed relative of the Nef-associated kinase, achieved a comparable result. Taken together, these data suggest that MA is a target of the Nef- associated serine kinase.


This article has been cited by other articles:

  • Park, I.-W., He, J. J. (2009). HIV-1 Nef-mediated inhibition of T cell migration and its molecular determinants. J. Leukoc. Biol. 86: 1171-1178 [Abstract] [Full Text]  
  • Laguette, N., Benichou, S., Basmaciogullari, S. (2009). Human Immunodeficiency Virus Type 1 Nef Incorporation into Virions Does Not Increase Infectivity. J. Virol. 83: 1093-1104 [Abstract] [Full Text]  
  • Smith, R. A., Anderson, D. J., Preston, B. D. (2006). Hypersusceptibility to Substrate Analogs Conferred by Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase. J. Virol. 80: 7169-7178 [Abstract] [Full Text]  
  • Smith, R. A., Anderson, D. J., Preston, B. D. (2004). Purifying Selection Masks the Mutational Flexibility of HIV-1 Reverse Transcriptase. J. Biol. Chem. 279: 26726-26734 [Abstract] [Full Text]  
  • Kaushik, R., Ratner, L. (2004). Role of Human Immunodeficiency Virus Type 1 Matrix Phosphorylation in an Early Postentry Step of Virus Replication. J. Virol. 78: 2319-2326 [Abstract] [Full Text]  
  • von Schwedler, U. K., Stray, K. M., Garrus, J. E., Sundquist, W. I. (2003). Functional Surfaces of the Human Immunodeficiency Virus Type 1 Capsid Protein. J. Virol. 77: 5439-5450 [Abstract] [Full Text]  
  • Dorfman, T., Popova, E., Pizzato, M., Gottlinger, H. G. (2002). Nef Enhances Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Matrix. J. Virol. 76: 6857-6862 [Abstract] [Full Text]  
  • Moroz, C., Traub, L., Maymon, R., Zahalka, M. A. (2002). PLIF, a Novel Human Ferritin Subunit from Placenta with Immunosuppressive Activity. J. Biol. Chem. 277: 12901-12905 [Abstract] [Full Text]  
  • Butler, S. L., Johnson, E. P., Bushman, F. D. (2002). Human Immunodeficiency Virus cDNA Metabolism: Notable Stability of Two-Long Terminal Repeat Circles. J. Virol. 76: 3739-3747 [Abstract] [Full Text]  
  • Khan, M., Garcia-Barrio, M., Powell, M. D. (2001). Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription. J. Virol. 75: 12081-12087 [Abstract] [Full Text]  
  • Schaeffer, E., Geleziunas, R., Greene, W. C. (2001). Human Immunodeficiency Virus Type 1 Nef Functions at the Level of Virus Entry by Enhancing Cytoplasmic Delivery of Virions. J. Virol. 75: 2993-3000 [Abstract] [Full Text]  
  • Liu, L. X., Heveker, N., Fackler, O. T., Arold, S., Le Gall, S., Janvier, K., Peterlin, B. M., Dumas, C., Schwartz, O., Benichou, S., Benarous, R. (2000). Mutation of a Conserved Residue (D123) Required for Oligomerization of Human Immunodeficiency Virus Type 1 Nef Protein Abolishes Interaction with Human Thioesterase and Results in Impairment of Nef Biological Functions. J. Virol. 74: 5310-5319 [Abstract] [Full Text]  
  • Smith, R. A., Klarmann, G. J., Stray, K. M., von Schwedler, U. K., Schinazi, R. F., Preston, B. D., North, T. W. (1999). A New Point Mutation (P157S) in the Reverse Transcriptase of Human Immunodeficiency Virus Type 1 Confers Low-Level Resistance to (-)-beta -2',3'-Dideoxy-3'-Thiacytidine. Antimicrob. Agents Chemother. 43: 2077-2080 [Abstract] [Full Text]  
  • Carteau, S., Gorelick, R. J., Bushman, F. D. (1999). Coupled Integration of Human Immunodeficiency Virus Type 1 cDNA Ends by Purified Integrase In Vitro: Stimulation by the Viral Nucleocapsid Protein. J. Virol. 73: 6670-6679 [Abstract] [Full Text]  
  • Mangasarian, A., Piguet, V., Wang, J.-K., Chen, Y.-L., Trono, D. (1999). Nef-Induced CD4 and Major Histocompatibility Complex Class I (MHC-I) Down-Regulation Are Governed by Distinct Determinants: N-Terminal Alpha Helix and Proline Repeat of Nef Selectively Regulate MHC-I Trafficking. J. Virol. 73: 1964-1973 [Abstract] [Full Text]  
  • Welker, R., Harris, M., Cardel, B., Krausslich, H.-G. (1998). Virion Incorporation of Human Immunodeficiency Virus Type 1 Nef Is Mediated by a Bipartite Membrane-Targeting Signal: Analysis of Its Role in Enhancement of Viral Infectivity. J. Virol. 72: 8833-8840 [Abstract] [Full Text]  
  • Carteau, S., Hoffmann, C., Bushman, F. (1998). Chromosome Structure and Human Immunodeficiency Virus Type 1 cDNA Integration: Centromeric Alphoid Repeats Are a Disfavored Target. J. Virol. 72: 4005-4014 [Abstract] [Full Text]  
  • Nie, Z., Bergeron, D., Subbramanian, R. A., Yao, X.-J., Checroune, F., Rougeau, N., Cohen, E. A. (1998). The Putative Alpha Helix 2 of Human Immunodeficiency Virus Type 1 Vpr Contains a Determinant Which Is Responsible for the Nuclear Translocation of Proviral DNA in Growth-Arrested Cells. J. Virol. 72: 4104-4115 [Abstract] [Full Text]  
  • D'Aloja, P., Olivetta, E., Bona, R., Nappi, F., Pedacchia, D., Pugliese, K., Ferrari, G., Verani, P., Federico, M. (1998). gag, vif, and nef Genes Contribute to the Homologous Viral Interference Induced by a Nonproducer Human Immunodeficiency Virus Type 1 (HIV-1) Variant: Identification of Novel HIV-1-Inhibiting Viral Protein Mutants. J. Virol. 72: 4308-4319 [Abstract] [Full Text]  
  • Gamble, T. R., Yoo, S., Vajdos, F. F., von Schwedler, U. K., Worthylake, D. K., Wang, H., McCutcheon, J. P., Sundquist, W. I., Hill, C. P. (1997). Structure of the Carboxyl-Terminal Dimerization Domain of the HIV-1 Capsid Protein. Science 278: 849-853 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»