This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goepfert, P. A.
Right arrow Articles by Mulligan, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goepfert, P. A.
Right arrow Articles by Mulligan, M. J.

 Previous Article  |  Next Article 

J. Virol., 01 1997, 778-784, Vol 71, No. 1
Copyright © 1997, American Society for Microbiology

A sorting motif localizes the foamy virus glycoprotein to the endoplasmic reticulum

PA Goepfert, KL Shaw, GD Ritter Jr and MJ Mulligan
Department of Medicine, University of Alabama at Birmingham, 35294- 2170, USA.

We recently identified an endoplasmic reticulum (ER) retrieval signal- the dilysine motif-in the glycoproteins of all five foamy viruses (FVs) for which sequences were available (P. A. Goepfert, G. Wang, and M. J. Mulligan, Cell 82:543-544, 1995). In the present study, expression of recombinant human FV (HFV) glycoprotein and analyses of oligosaccharide modifications and precursor cleavage indicated that the protein was localized to the ER. HFV glycoproteins encoding seven different dilysine motif mutations were then expressed. The results indicated that disruptions of the dilysine motif resulted in higher levels of forward transport of the HFV glycoprotein from the ER through the Golgi apparatus to the plasma membrane. We conclude that the dilysine motif is responsible for ER sorting of the FV glycoprotein. Signal-mediated ER localization has not previously been described for a retroviral glycoprotein.


This article has been cited by other articles:

  • Stange, A., Luftenegger, D., Reh, J., Weissenhorn, W., Lindemann, D. (2008). Subviral Particle Release Determinants of Prototype Foamy Virus. J. Virol. 82: 9858-9869 [Abstract] [Full Text]  
  • Lontok, E., Corse, E., Machamer, C. E. (2004). Intracellular Targeting Signals Contribute to Localization of Coronavirus Spike Proteins near the Virus Assembly Site. J. Virol. 78: 5913-5922 [Abstract] [Full Text]  
  • Verschoor, E. J., Langenhuijzen, S., van den Engel, S., Niphuis, H., Warren, K. S., Heeney, J. L. (2003). Structural and Evolutionary Analysis of an Orangutan Foamy Virus. J. Virol. 77: 8584-8587 [Abstract] [Full Text]  
  • Shikova-Lekova, E., Lindemann, D., Pietschmann, T., Juretzek, T., Rudolph, W., Herchenroder, O., Gelderblom, H. R., Rethwilm, A. (2003). Replication-Competent Hybrids between Murine Leukemia Virus and Foamy Virus. J. Virol. 77: 7677-7681 [Abstract] [Full Text]  
  • Picard-Maureau, M., Jarmy, G., Berg, A., Rethwilm, A., Lindemann, D. (2003). Foamy Virus Envelope Glycoprotein-Mediated Entry Involves a pH-Dependent Fusion Process. J. Virol. 77: 4722-4730 [Abstract] [Full Text]  
  • Shaw, K. L., Lindemann, D., Mulligan, M. J., Goepfert, P. A. (2003). Foamy Virus Envelope Glycoprotein Is Sufficient for Particle Budding and Release. J. Virol. 77: 2338-2348 [Abstract] [Full Text]  
  • Lecellier, C.-H., Neves, M., Giron, M.-L., Tobaly-Tapiero, J., Saib, A. (2002). Further Characterization of Equine Foamy Virus Reveals Unusual Features among the Foamy Viruses. J. Virol. 76: 7220-7227 [Abstract] [Full Text]  
  • Eastman, S. W., Linial, M. L. (2001). Identification of a Conserved Residue of Foamy Virus Gag Required for Intracellular Capsid Assembly. J. Virol. 75: 6857-6864 [Abstract] [Full Text]  
  • Lindemann, D., Pietschmann, T., Picard-Maureau, M., Berg, A., Heinkelein, M., Thurow, J., Knaus, P., Zentgraf, H., Rethwilm, A. (2001). A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and Infectivity. J. Virol. 75: 5762-5771 [Abstract] [Full Text]  
  • Meiering, C. D., Maxine L. Linial, (2001). Historical Perspective of Foamy Virus Epidemiology and Infection. Clin. Microbiol. Rev. 14: 165-176 [Abstract] [Full Text]  
  • Pietschmann, T., Zentgraf, H., Rethwilm, A., Lindemann, D. (2000). An Evolutionarily Conserved Positively Charged Amino Acid in the Putative Membrane-Spanning Domain of the Foamy Virus Envelope Protein Controls Fusion Activity. J. Virol. 74: 4474-4482 [Abstract] [Full Text]  
  • Tobaly-Tapiero, J., Bittoun, P., Neves, M., Guillemin, M.-C., Lecellier, C.-H., Puvion-Dutilleul, F., Gicquel, B., Zientara, S., Giron, M.-L., de Thé, H., Saïb, A. (2000). Isolation and Characterization of an Equine Foamy Virus. J. Virol. 74: 4064-4073 [Abstract] [Full Text]  
  • Heinkelein, M., Thurow, J., Dressler, M., Imrich, H., Neumann-Haefelin, D., McClure, M. O., Rethwilm, A. (2000). Complex Effects of Deletions in the 5' Untranslated Region of Primate Foamy Virus on Viral Gene Expression and RNA Packaging. J. Virol. 74: 3141-3148 [Abstract] [Full Text]  
  • Ono, A., Orenstein, J. M., Freed, E. O. (2000). Role of the Gag Matrix Domain in Targeting Human Immunodeficiency Virus Type 1 Assembly. J. Virol. 74: 2855-2866 [Abstract] [Full Text]  
  • Bansal, A., Shaw, K. L., Edwards, B. H., Goepfert, P. A., Mulligan, M. J. (2000). Characterization of the R572T Point Mutant of a Putative Cleavage Site in Human Foamy Virus Env. J. Virol. 74: 2949-2954 [Abstract] [Full Text]  
  • Meiering, C. D., Comstock, K. E., Linial, M. L. (2000). Multiple Integrations of Human Foamy Virus in Persistently Infected Human Erythroleukemia Cells. J. Virol. 74: 1718-1726 [Abstract] [Full Text]  
  • Vincent, M. J., Melsen, L. R., Martin, A. S., Compans, R. W. (1999). Intracellular Interaction of Simian Immunodeficiency Virus Gag and Env Proteins. J. Virol. 73: 8138-8144 [Abstract] [Full Text]  
  • Wang, G., Mulligan, M. J., Baldwin, D. N., Linial, M. L. (1999). Endogenous Virus of BHK-21 Cells Complicates Electron Microscopy Studies of Foamy Virus Maturation. J. Virol. 73: 8917-8917 [Full Text]  
  • Goepfert, P. A., Shaw, K., Wang, G., Bansal, A., Edwards, B. H., Mulligan, M. J. (1999). An Endoplasmic Reticulum Retrieval Signal Partitions Human Foamy Virus Maturation to Intracytoplasmic Membranes. J. Virol. 73: 7210-7217 [Abstract] [Full Text]  
  • Hill, C. L., Bieniasz, P. D., McClure, M. O. (1999). Properties of human foamy virus relevant to its development as a vector for gene therapy. J. Gen. Virol. 80: 2003-2009 [Abstract] [Full Text]  
  • Pietschmann, T., Heinkelein, M., Heldmann, M., Zentgraf, H., Rethwilm, A., Lindemann, D. (1999). Foamy Virus Capsids Require the Cognate Envelope Protein for Particle Export. J. Virol. 73: 2613-2621 [Abstract] [Full Text]  
  • Linial, M. L. (1999). Foamy Viruses Are Unconventional Retroviruses. J. Virol. 73: 1747-1755 [Full Text]  
  • Wang, G, Mulligan, M. (1999). Comparative sequence analysis and predictions for the envelope glycoproteins of foamy viruses. J. Gen. Virol. 80: 245-254 [Abstract]  
  • Mochizuki, H., Schwartz, J. P., Tanaka, K., Brady, R. O., Reiser, J. (1998). High-Titer Human Immunodeficiency Virus Type 1-Based Vector Systems for Gene Delivery into Nondividing Cells. J. Virol. 72: 8873-8883 [Abstract] [Full Text]  
  • Salzwedel, K., West, J. T. Jr., Mulligan, M. J., Hunter, E. (1998). Retention of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein in the Endoplasmic Reticulum Does Not Redirect Virus Assembly from the Plasma Membrane. J. Virol. 72: 7523-7531 [Abstract] [Full Text]  
  • Giron, M.-L., de The, H., Saib, A. (1998). An Evolutionarily Conserved Splice Generates a Secreted Env-Bet Fusion Protein during Human Foamy Virus Infection. J. Virol. 72: 4906-4910 [Abstract] [Full Text]  
  • Baldwin, D. N., Linial, M. L. (1998). The Roles of Pol and Env in the Assembly Pathway of Human Foamy Virus. J. Virol. 72: 3658-3665 [Abstract] [Full Text]  
  • Lindemann, D., Rethwilm, A. (1998). Characterization of a Human Foamy Virus 170-Kilodalton Env-Bet Fusion Protein Generated by Alternative Splicing. J. Virol. 72: 4088-4094 [Abstract] [Full Text]  
  • Fischer, N., Heinkelein, M., Lindemann, D., Enssle, J., Baum, C., Werder, E., Zentgraf, H., Muller, J. G., Rethwilm, A. (1998). Foamy Virus Particle Formation. J. Virol. 72: 1610-1615 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»